5 research outputs found
HISTOCHEMICAL STUDIES OF HIDES IN PROCESS FROM FRESH SKINS TO TANNED LEATHERS (REPORT III), ESPECIALLY THE SWELLING AND SPLITTING OF COLLAGENOLTS FIBERS, AND THE MECHANISM OF SUFFICIENT VENTILATION IN LEATHERS
PHYSIO-HISTOLOGICAL STUDIES ON THE PHYSIOLOGICAL OBESITY OF MEAT PIGS, (REPORT IX), ESPECIALLY ON THE OCCURRENCE OF GLYCOGEN WITHIN THE NUCLEI AND CYTOPLASMS OF THE ZONA RETICULARIS IN THE ADRENALS OF WILD BOARS AND LANDRACE MEAT PIGS IN COMPARISON WITH THAT OF YORKSHIRE-AND LANDRACE F_1-MEAT PIGS, YORKSHIRE PIGLETS, YORKSHIRE PIGS IMMUNIZED WITH HOG-CHOLERA VIRUS, AND OTHER PIGS
HISTOCHEMICAL STUDIES OF HIDES IN PROCESS FROM FRESH SKINS TO TANNED LEATHERS (REPORT III), ESPECIALLY THE SWELLING AND SPLITTING OF COLLAGENOLTS FIBERS, AND THE MECHANISM OF SUFFICIENT VENTILATION IN LEATHERS
Development of novel 191Pt-labeled Hoechst33258: 191Pt is more suitable than 111In for targeting DNA
Abstract:This study aims to establish new labeling methods for no-carrier–added radio-Pt (191Pt) and to evaluate the in vitro properties of 191Pt-labeled agents compared with those of agents labeled with the common emitter 111In. 191Pt was complexed with the DNA-targeting dye Hoechst33258 via diethylenetriaminepentaacetic acid (DTPA) or the sulfur-containing amino acid cysteine (Cys). The intranuclear fractions of 191Pt- and 111In-labeled Hoechst33258 were comparable, indicating that the labeling for 191Pt via DTPA or Cys and the labeling for 111In via DTPA worked equally well. 191Pt showed a DNA-binding/cellular uptake ratio more than one order of magnitude greater than that of 111In. [191Pt]Pt-Hoechst33258 labeled via Cys showed a higher cellular uptake than that labeled via DTPA, resulting in a very high DNA-binding fraction of [191Pt]Pt-Cys-Hoechst33258 and extensive DNA damage. Our labeling methods of radio-Pt, especially via Cys, promote the development of radio-Pt-based agents for use in Auger electron therapy targeting DNA