Is thyroid stimulating hormone titration mandatory in the routine work-up of African males from infertile couples?

Background: The impact of thyroid hormones on male reproductive function is still insufficiently understood. The objectives of this study were to determine the frequency of thyroid dysfunction in men from infertile couples and to establish an association between TSH (thyroid stimulating hormone) values and sperm parameters.Methods: We conducted a cross-sectional analytical study on a consecutive series of men managed for couple infertility in two reference hospitals of Yaoundé from November 2017 to May 2018. For each participant, a questionnaire was administered, TSH was assayed using electro-chemo-luminescence and sperm analyzed. Statistical methods used were the Mann-Whitney test and the Spearman correlation coefficient with a significance threshold of 5%.Results: Overall, 123 men were recruited. The median age was 44 years old [38-50 years]; 60 (48.79%) patients had couple infertility lasting between 1 and 5 years. Primary infertility of the couple was the most common (82 cases, 66.70%). After sperm analysis, 86 patients (70%) had one or more anomalies. TSH titration revealed 03 (2.44%) cases of subclinical hyperthyroidism, 05 (4.06%) subclinical hypothyroidism and 115 (93.50%) euthyroidism. Significant associations were found between TSH and sperm parameters in the group of patients with thyroid dysfunction, but none in the euthyroid group.Conclusions: Frequency of dysthyroidism is low among African males from infertile couples. We noted significant correlations between TSH values and sperm parameters. The low frequency of thyroid dysfunction would be against a TSH titration in the routine work-up of these patients

Nanocolumnar TiN thin film growth by oblique angle sputter-deposition: Experiments vs. simulations

Nanostructured columnar titanium nitride (TiN) thin films were produced by oblique angle deposition using reactive magnetron sputtering. The influence of the angular distribution of the incoming particle flux on the resulting filmmorphology (columntilt angle, porosity, surface roughness) was studied by varying the inclination angle α of the substrate at two different working pressures, 0.3 and 0.5 Pa. The microstructural features and columns tilt angles βexp determined experimentally were compared to those simulated from two kinetic Monte Carlo (KMC) models. With increasing pressure, the TiN columns were found to be less defined but no significant changes in βexp were revealed. Both KMC models satisfactorily reproduced the experimental findings, the agreement being closer at 0.5 Pa. The evolution of β angle is also discussed with respect to the resulting incidence angle θres of the incoming flux, this latter quantity accounting for the local incidence angle of individual particles,which may greatly differ fromthe geometrical angle α, especially at highworking pressure due to the incoming particle – gas collisions. Crossover phenomena between the 0.3 and 0.5 Pa series were revealed from the evolution of the film resistivity, as well as simulated layer density and surface roughness versus α angle.This work has been performed within the M.ERA-NET project MC2 “Multi-scale Computational-driven design of novel hard nanostructured Coatings” and funded by the French ANR program (Project No. ANR-13-MERA-0002-02). BB acknowledges the financial support from the Algerian Ministry of Higher Education and Scientific Research through the grant n°173 of the PNE 2016-17 program

A comparative analysis of SLA-DRB1 genetic diversity in Colombian (creoles and commercial line) and worldwide swine populations

Analysing pig class II mayor histocompatibility complex (MHC) molecules is mainly related to antigen presentation. Identifying frequently-occurring alleles in pig populations is an important aspect to be considered when developing peptide-based vaccines. Colombian creole pig populations have had to adapt to local conditions since entering Colombia; a recent census has shown low amounts of pigs which is why they are considered protected by the Colombian government. Commercial hybrids are more attractive regarding production. This research has been aimed at describing the allele distribution of Colombian pigs from diverse genetic backgrounds and comparing Colombian SLA-DRB1 locus diversity to that of internationally reported populations. Twenty SLA-DRB1 alleles were identified in the six populations analysed here using sequence-based typing. The amount of alleles ranged from six (Manta and Casco Mula) to nine (San Pedreño). Only one allele (01:02) having > 5% frequency was shared by all three commercial line populations. Allele 02:01:01 was shared by five populations (around > 5% frequency). Global FST indicated that pig populations were clearly structured, as 20.6% of total allele frequency variation was explained by differences between populations (FST = 0.206). This study’s results confirmed that the greatest diversity occurred in wild boars, thereby contrasting with low diversity in domestic pig populations.This work was supported by the Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A)

The full-ORF clone resource of the German cDNA Consortium

<p>Abstract</p> <p>Background</p> <p>With the completion of the human genome sequence the functional analysis and characterization of the encoded proteins has become the next urging challenge in the post-genome era. The lack of comprehensive ORFeome resources has thus far hampered systematic applications by protein gain-of-function analysis. Gene and ORF coverage with full-length ORF clones thus needs to be extended. In combination with a unique and versatile cloning system, these will provide the tools for genome-wide systematic functional analyses, to achieve a deeper insight into complex biological processes.</p> <p>Results</p> <p>Here we describe the generation of a full-ORF clone resource of human genes applying the Gateway cloning technology (Invitrogen). A pipeline for efficient cloning and sequencing was developed and a sample tracking database was implemented to streamline the clone production process targeting more than 2,200 different ORFs. In addition, a robust cloning strategy was established, permitting the simultaneous generation of two clone variants that contain a particular ORF with as well as without a stop codon by the implementation of only one additional working step into the cloning procedure. Up to 92 % of the targeted ORFs were successfully amplified by PCR and more than 93 % of the amplicons successfully cloned.</p> <p>Conclusion</p> <p>The German cDNA Consortium ORFeome resource currently consists of more than 3,800 sequence-verified entry clones representing ORFs, cloned with and without stop codon, for about 1,700 different gene loci. 177 splice variants were cloned representing 121 of these genes. The entry clones have been used to generate over 5,000 different expression constructs, providing the basis for functional profiling applications. As a member of the recently formed international ORFeome collaboration we substantially contribute to generating and providing a whole genome human ORFeome collection in a unique cloning system that is made freely available in the community.</p

Auditoria forense como servicio especializado para la prevención del fraude

The objective of this research proposal is to study forensic auditing as a specialized service for fraud prevention, based on the authors Cano, D., Lugo, D., Cano, M., &amp; Cano, J. (2009), ACFE (2022), Mendoza (2009), among others. For the design of this proposal, documents such as scientific research articles, official statistics, degree works were consulted, which refer to the study variable framed in the forensic audit and its effect on the detection of fraud. Methodologically, it is a descriptive study under a qualitative approach, consisting of a field design and a documentary study. The expected results will allow us to answer the problem question raised on how the forensic audit as a specialized service helps to prevent fraud? Likewise, it is expected to generate relevant conclusions in response to the specific objectives formulated in the design of this research proposal. with the intention of responding to the general objective and proposing recommendations based on the study, if necessary.El objetivo de esta propuesta de investigación es estudiar la auditoria forense como servicio especializado para la prevención del fraude, basado en los autores Cano, D., Lugo, D., Cano, M., &amp; Cano, J. (2009), ACFE (2022), Mendoza (2009), entre otros. Para el &nbsp;&nbsp;&nbsp;diseño de esta propuesta se consultó documentos como artículos de investigación científica, estadísticas oficiales, trabajos de grado, que referencian la variable de estudio enmarcadas en la auditoria forense y su efecto en la detección de fraudes. Metodológicamente, se trata de un estudio descriptivo bajo un enfoque cualitativo, constituido por un diseño de campo y estudio documental. Los resultados esperados permitirán dar respuesta a la pregunta problema planteada sobre ¿cómo la auditoria forense como servicio especializado ayuda a la prevención del fraude?, así mismo se espera generar conclusiones pertinentes en atención a los objetivos específicos formulados en el diseño de esta propuesta investigativa, con intenciones de responder al objetivo general y proponer recomendaciones a partir del estudio de ser necesario

In-Space Utilisation of Asteroids::“Answers to Questions from the Asteroid Miners”

The aim of the Asteroid Science Intersections with In-­Space Mine Engineering (ASIME) 2016 conference on September 21-­‐22, 2016 in Luxembourg City wasto provide an environment for the detailed discussion of the specific properties of asteroids, with the engineering needs of space missions that utilize asteroids.The ASIME 2016 Conference produced a layered record of discussions from theasteroid scientists and the asteroid miners to understand each other’s key concerns and to address key scientific questions from the asteroid mining companies: Planetary Resources, Deep Space Industries and TransAstra. These Questions were the focus of the two day conference, were addressed byscientists inside and outside of the ASIME Conference and are the focus ofthis White Paper.The Questions from the asteroid mining companies have been sorted into the three asteroid science themes: 1) survey, 2) surface and 3) subsurface and 4)Other. The answers to those Questions have been provided by the scientists with their conference presentations or edited directly into an early open-­‐access collaborative Google document (August 2016-­‐October 2016), or inserted byA. Graps using additional reference materials. During the ASIME 2016 last two-­‐hours, the scientists turned the Questions from the Asteroid Miners around by presenting their own key concerns: Questions from the Asteroid Scientists. These answers in this White Paper will point to the Science Knowledge Gaps (SKGs) for advancing the asteroid in-­‐space resource utilisation domain