46 research outputs found
DEVELOPMENT OF UNIVERSITY TEACHER’S INNOVATIVE CULTURE
Purpose: The aim of research is to analyze the development of university teacher’s innovative culture.
Methodology: This is an analytical-descriptive research that obtained data from questionnaires and interviews. Content analysis and model structure have been used to analyze the data.
Main Findings: Results showed that the formed teacher’s innovative space brings a future specialist to the understanding that the quality of life depends on the laborious work on one’s own spirituality, morality and worldview. The future specialist gets experience of professional activity on the basis of universal humane values, which is the main result of the modeled innovative culture in the pedagogical space.
Applications: This research can be used by teachers, the education system and graduate students.
Novelty/Originality: For the first time, modelling the innovation process of teachers has been studied
LEGAL CULTURE FORMATION OF A FUTURE SPECIALIST
Purpose: To identify Legal Culture Formation of a Future Specialist.
Methodology: This is an analytical-critical research that uses content analysis and interviews to gain data. In this re-search, all the information obtained are coded, then meaningful propositions were specified and finally a list of these factors are provided.
Main Findings: Results showed that knowledge about the foundations of law in the modern times becomes the primary necessity of every member in the society, including students of professional educational institutions, as the success of people in any sphere of their activity largely depends on this: in business, when working in enterprises for hiring new talents or in budgetary organizations, medical care, etc.
Applications: The research implications can be used by legal training professional educational institutions and universities.
Novelty/Originality: For the first time, hours devoted to the study of law subjects; unsatisfactory qualification of teachers in the legal course; lack of systematic educational and methodical literature, and documentation has been studied
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Declining resilience of ecosystem functions under biodiversity loss
The composition of species communities is changing rapidly through drivers such as habitat loss and climate change, with potentially serious consequences for the resilience of ecosystem functions on which humans depend. To assess such changes in resilience, we analyse trends in the frequency of species in Great Britain that provide key ecosystem functions-specifically decomposition, carbon sequestration, pollination, pest control and cultural values. For 4,424 species over four decades, there have been significant net declines among animal species that provide pollination, pest control and cultural values. Groups providing decomposition and carbon sequestration remain relatively stable, as fewer species are in decline and these are offset by large numbers of new arrivals into Great Britain. While there is general concern about degradation of a wide range of ecosystem functions, our results suggest actions should focus on particular functions for which there is evidence of substantial erosion of their resilience
The waking brain: an update
Wakefulness and consciousness depend on perturbation of the cortical soliloquy. Ascending activation of the cerebral cortex is characteristic for both waking and paradoxical (REM) sleep. These evolutionary conserved activating systems build a network in the brainstem, midbrain, and diencephalon that contains the neurotransmitters and neuromodulators glutamate, histamine, acetylcholine, the catecholamines, serotonin, and some neuropeptides orchestrating the different behavioral states. Inhibition of these waking systems by GABAergic neurons allows sleep. Over the past decades, a prominent role became evident for the histaminergic and the orexinergic neurons as a hypothalamic waking center
Internal (His)6-tagging delivers a fully functional hetero-oligomeric class II chaperonin in high yield
Group II chaperonins are ATP-ases indispensable for the folding of many proteins that play a crucial role in Archaea and Eukarya. They display a conserved two-ringed assembly enclosing an internal chamber where newly translated or misfolded polypeptides can fold to their native structure. They are mainly hexadecamers, with each eight-membered ring composed of one or two (in Archaea) or eight (in Eukarya) different subunits. A major recurring problem within group II chaperonin research, especially with the hetero-oligomeric forms, is to establish an efficient recombinant system for the expression of large amounts of wild-type as well as mutated variants. Herein we show how we can produce, in E. coli cells, unprecedented amounts of correctly assembled and active αβ-thermosome, the class II chaperonin from Thermoplasma acidophilum, by introducing a (His)6-tag within a loop in the α subunit of the complex. The specific location was identified via a rational approach and proved not to disturb the structure of the chaperonin, as demonstrated by size-exclusion chromatography, native gel electrophoresis and electron microscopy. Likewise, the tagged protein showed an ATP-ase activity and an ability to refold substrates identical to the wild type. This tagging strategy might be employed for the overexpression of other recombinant chaperonins