Tailoring 3D single-walled carbon nanotubes anchored to indium tin oxide for natural cellular uptake and intracellular sensing.

The ability to monitor intracellular events in real time is paramount to advancing fundamental biological and clinical science. We present the first demonstration of a direct interface of vertically aligned single-walled carbon nanotubes (VASWCNTs) with eukaryotic cells, RAW 264.7 mouse macrophage cell line. The cells were cultured on indium tin oxide with VASWCNTs. VASWCNTs entered the cells naturally without application of any external force and were shown to sense the intracellular presence of a redox active moiety, methylene blue. The technology developed provides an alluring platform to enable electrochemical study of an intracellular environment

Production of lab scale limestone calcined clay cements using low grade limestone

In developing countries, such as India, cement production is expected to rise exponentially in the coming decades with the increasing infrastructure demand. Sustainable alternatives are being looked into to account for the growing demand and to reduce the CO2 emissions. Limestone calcined clay cement (LC3) with clinker factor of 0.5 is one of the sustainable ways to reduce the carbon footprint and meet the growing demand. In this study, clay from a particular source having a kaolinite content of 65% is selected and calcined at ~800º C in a laboratory scale rotary kiln. The calcination parameters are fixed to obtain a uniform flow in the kiln. Three different qualities of limestone with low calcium carbonate content are selected from rejected limestone dumps. LC3 blends are prepared by blending calcined clay, limestone and clinker in a lab scale ball mill using different limestone grades. The tests on physical properties of the blends were carried out and compared with the properties of fly ash based Porltand pozzolana cement. The properties of the blends are found satisfactory. The phases formed in the hydrated specimens are found using X-ray diffraction. Compressive strength and formation of phases in the hydrated paste are monitored till 90 days

Effect of alkali-hydroxide on hydration kinetics and microstructure of high-volume fly ash blended cement pastes

202408 bcchNot applicableRGCPublished24 monthsGreen (AAM