New Insights into Metabolic Properties of Marine Bacteria Encoding Proteorhodopsins

Proteorhodopsin phototrophy was recently discovered in oceanic surface waters. In an effort to characterize uncultured proteorhodopsin-exploiting bacteria, large-insert bacterial artificial chromosome (BAC) libraries from the Mediterranean Sea and Red Sea were analyzed. Fifty-five BACs carried diverse proteorhodopsin genes, and we confirmed the function of five. We calculate that proteorhodopsin-exploiting bacteria account for 13% of microorganisms in the photic zone. We further show that some proteorhodopsin-containing bacteria possess a retinal biosynthetic pathway and a reverse sulfite reductase operon, employed by prokaryotes oxidizing sulfur compounds. Thus, these novel phototrophs are an unexpectedly large and metabolically diverse component of the marine microbial surface water

INFLUENCE OF GRAIN TYPE AND OIL CONCENTRATION OF DRIED CORN DISTILLERS GRAIN WITH SOLUBLES ON RUMINAL FERMENTATION AND IN VITRO GAS PRODUCTION IN CATTLE FED HIGH-CONCENTRATE DIETS

To determine the effects of grain type (rolled-corn vs rolled-barley) and dried corn distillers grains with solubles (DDGS) oil concentration (low = 4.5 % vs moderate = 7.9 %) on ruminal pH, ammonia (NH3) and volatile fatty acid (VFA) concentrations, in vitro enteric methane (CH4) and carbon dioxide (CO2) production, and digestive enzyme activity, eight ruminally cannulated Holstein steers (715 ± 61.4 kg) were used in a 4 × 8 Latin rectangle with a 2 × 2 factorial arrangement of treatments. Diets were offered for ad libitum intake. Experimental periods were 24 d with 7 d of diet adaptation, 7 d of sample collection, and a 10-d transition between periods. No differences were observed for ruminal NH3, total VFA concentration, pH, or in vitro concentration of CH4 or CO2 after 24 hr of incubation among treatments. The lag time for in vitro gas production and degradation was greater (P = 0.03) from steers fed diets containing rolled-corn than rolled-barley. There were grain type × DDGS interactions (P ≤ 0.02) observed for α-amylase and trypsin activity (U/L ruminal fluid). Maltase activity (U/L ruminal fluid) was greater (P ≤ 0.01) in steers fed diets containing rolled-corn than rolled-barley and in steers fed diets containing low- compared to moderate-oil DDGS. These results indicate that, although ruminal enzyme activity was influenced, feeding different grain types (rolled-corn vs. rolled-barley) or DDGS with differing oil concentration (moderate vs. low) did not negatively influence ruminal fermentation or in vitro CH4 production.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

The application of signature character strengths and positive experiences at work

We hypothesized that the amount of positive experiences at work (job satis- faction, pleasure, engagement, meaning) is a function of the extent to which the situational circumstances at the workplace allow for the application of an individual’s signature character strengths. For the description of the individual a reliable and valid instrument already exists, but not for the environment. Hence, the newly developed Applicability of Character Strengths Rating Scales (ACS-RS) with information on its reliability and validity were also presented. A sample of 1,111 adults filled in the ACS-RS and measures for possession of character strengths and positive experiences at work. The ACS-RS was reliable by means of internal consistency and inter-rater reliability. It proved to be valid in several ways being sensitive to: (a) the differences in the applicability of trait-relevant behavior in formal versus informal situations by showing higher applicability of the character strengths in the latter; (b) the differences between traits regarding their appli- cability across situations; (c) people’s disposition to choose situations fitting their dispo- sitions by showing positive relationships between the degree of possession and applicability. Moreover, correlations between applicability of strengths and positive experiences increased with the individual centrality of the strengths. The more signature strengths were applied at the workplace, the higher the positive experiences at work. This study showed that character strengths matter in vocational environments irrespective of their content. Strengths-congruent activities at the workplace are important for positive experiences at work like job satisfaction and experiencing pleasure, engagement, and meaning fostered by one’s job