Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants

We have developed a new, simple, quick and genotype-independent method for direct regeneration of sugarcane using novel midrib segment explants. Our protocol involves two steps: the pretreatment of starting material on MS (Murashige and Skoog (1962) Physiol Plant 15:473–497) medium containing 3.0 mg/l 2,4- dichlorophenoxyacetic acid (2,4-D) for 8 days under continuous dark and subsequent transfer of the explants to MS medium augmented with 0.1 mg/l benzyladenine (BA) and 0.1 mg/l naphthaleneacetic acid (NAA) under light-dark conditions. On the regeneration medium, numerous globular structures appeared from the explants and subsequently differentiated into shoots. Regenerated shoots attained 2–5 cm height within 30 days of culture initiation and readily rooted on MS basal medium. Hardened plants were successfully established in the greenhouse. The regulation of sugarcane morphogenesis by auxin pretreatment is discussed

Rational drug design applied to myeloperoxidase inhibition

Rational drug design is a general approach using protein-structure technique in which the discovery of a ligand can be driven either by chance, screening, or rational theory. Myeloperoxidase (MPO) was rapidly identified as a therapeutical target because of its involvement in chronic inflammatory syndromes. In this context, the research of MPO inhibitors was intensified and development of new chemical entities was rationally driven by the research of ligands that enter into the MPO catalytic pocket. Actually, as soon as crystallography data of MPO have become available and its structure was virtually designed, the rational drug design has been applied to this peroxidase. Pharmaceutical industries and academic laboratories apply rational drug design on MPO by either optimizing known inhibitors or searching new molecules by high-throughput virtual screening. By these ways, they were able to find efficient MPO inhibitors and understand their interactions with the enzyme. During this quest of MPO inhibition, it appears that Glu268 is a crucial residue in order to optimize ligandtarget interaction. This amino acid should be carefully considered by medicinal chemist when they design inhibitors interfering with MPO activity.SCOPUS: re.jinfo:eu-repo/semantics/publishe

Foreign policy, bipartisanship and the paradox of post-September 11 America

The attacks of September 11 and the resulting war on terrorism present a puzzle to conventional explanations of foreign policy bipartisanship. Public anxiety about the international environment increased sharply after the attacks in 2001, but this did not translate into greater foreign policy consensus despite the initial predictions of many analysts. In this article, we advance a theory of foreign policy bipartisanship that emphasizes its domestic underpinnings to explain the absence of consensus in Washington. We argue that bipartisanship over foreign policy depends as much on domestic economic and electoral conditions as on the international security environment. Using multivariate analysis of roll call voting in the House of Representatives from 1889 to 2008, we show that bipartisanship over foreign policy is most likely not only when the country faces a foreign threat but also when the national economy is strong and when party coalitions are regionally diverse. This was the case during the Cold War. Despite concern about terrorism in recent years, economic volatility and regional polarization have made bipartisan cooperation over foreign policy elusive