Inducing Ni Sensitivity in the Ni Hyperaccumulator Plant Alyssum inflatum Nyárády (Brassicaceae) by Transforming with CAX1, a Vacuolar Membrane Calcium Transporter

The importance of calcium in nickel tolerance was studied in the nickel hyperaccumulator plant Alyssum inflatum by gene transformation of CAX1, a vacuolar membrane transporter that reduces cytosolic calcium. CAX1 from Arabidopsis thaliana with a CaMV35S promoter accompanying a kanamycin resistance gene was transferred into A. inflatum using Agrobacterium tumefaciens. Transformed calli were subcultured three times on kanamycin-rich media and transformation was confirmed by PCR using a specific primer for CAX1. At least 10 callus lines were used as a pool of transformed material. Both transformed and untransformed calli were treated with varying concentrations of either calcium (1–15 mM) or nickel (0– 500 lM) to compare their responses to those ions. Increased external calcium generally led to increased callus biomass, however, the increase was greater for untransformed callus. Further, increased external calcium led to increased callus calcium concentrations. Transformed callus was less nickel tolerant than untransformed callus: under increasing nickel concentrations callus relative growth rate was significantly less for transformed callus. Transformed callus also contained significantly less nickel than untransformed callus when exposed to the highest external nickel concentration (200 lM). We suggest that transformation with CAX1 decreased cytosolic calcium and resulted in decreased nickel tolerance. This in turn suggests that, at low cytosolic calcium concentrations, other nickel tolerance mechanisms (e.g., complexation and vacuolar sequestration) are insufficient for nickel tolerance. We propose that high cytosolic calcium is an important mechanism that results in nickel tolerance by nickel hyperaccumulator plants

Management of rheumatoid arthritis: consensus recommendations from the Hong Kong Society of Rheumatology

Given the recent availability of novel biologic agents for the treatment of rheumatoid arthritis (RA), the Hong Kong Society of Rheumatology has developed consensus recommendations on the management of RA, which aim at providing guidance to local physicians on appropriate, literature-based management of this condition, specifically on the indications and monitoring of the biologic disease-modifying anti-rheumatic drugs (DMARDs). The recommendations were developed using the European League Against Rheumatism (EULAR) recommendations for the management of early arthritis as a guide, along with local expert opinion. As significant joint damage occurs early in the course of RA, initiating therapy early is key to minimizing further damage and disability. Patients with serious disease or poor prognosis should receive early, aggressive therapy. Because of its good efficacy and safety profile, methotrexate is considered the standard first-line DMARD for most treatment-naïve RA patients. Patients with a suboptimal response to methotrexate monotherapy should receive step-up (combination) therapy with either the synthetic or biologic DMARDs. In recent years, combinations of methotrexate with tocilizumab, abatacept, or rituximab have emerged as effective therapies in patients who are unresponsive to traditional DMARDs or the anti-tumor necrosis factor (TNF)-α agents. As biologic agents can increase the risk of infections such as tuberculosis and reactivation of viral hepatitis, screening for the presence of latent tuberculosis and chronic viral hepatitis carrier state is recommended before initiating therapy

The Imaging X-ray Polarimetry Explorer (IXPE) at last!

IXPE, the first observatory dedicated to imaging X-ray polarimetry, was launched on Dec 9, 2021 and is operating successfully. A partnership between NASA and the Italian Space Agencey (ASI) IXPE features three X-ray telescopes each comprised of a mirror module assembly with a polarization sensitive detector at its focus. An extending boom was deployed on orbit to provide the necessary 4 m focal length. A 3-axis-stabilized spacecraft provides power, attitude determination and control, and commanding. After one year of observation IXPE has measured statistically-significant polarization from almost all the classes of celestial sources that emit X-rays. In the following we describe the IXPE mission, reporting on its performance after 1.5 year of operations. We show the main astrophysical results which are outstanding for a SMEX mission

Highly Significant Detection of X-Ray Polarization from the Brightest Accreting Neutron Star Sco X-1

The Imaging X-ray Polarimetry Explorer measured with high significance the X-ray polarization of the brightest Z-source, Sco X-1, resulting in the nominal 2–8 keV energy band in a polarization degree of 1.0% ± 0.2% and a polarization angle of 8° ± 6° at a 90% confidence level. This observation was strictly simultaneous with observations performed by NICER, NuSTAR, and Insight-HXMT, which allowed for a precise characterization of its broadband spectrum from soft to hard X-rays. The source has been observed mainly in its soft state, with short periods of flaring. We also observed low-frequency quasiperiodic oscillations. From a spectropolarimetric analysis, we associate a polarization to the accretion disk at <3.2% at 90% confidence level, compatible with expectations for an electron scattering dominated optically thick atmosphere at the Sco X-1 inclination of ∼44°; for the higher-energy Comptonized component, we obtain a polarization of 1.3% ± 0.4%, in agreement with expectations for a slab of Thomson optical depth of ∼7 and an electron temperature of ∼3 keV. A polarization rotation with respect to previous observations by OSO-8 and PolarLight, and also with respect to the radio-jet position angle, is observed. This result may indicate a variation of the polarization with the source state that can be related to relativistic precession or a change in the corona geometry with the accretion flow

Discovery of X-Ray Polarization from the Black Hole Transient Swift J1727.8−1613

We report the first detection of the X-ray polarization of the bright transient Swift J1727.8−1613 with the Imaging X-ray Polarimetry Explorer. The observation was performed at the beginning of the 2023 discovery outburst, when the source resided in the bright hard state. We find a time- and energy-averaged polarization degree of 4.1% ± 0.2% and a polarization angle of 2.°2 ± 1.°3 (errors at 68% confidence level; this translates to ∼20σ significance of the polarization detection). This finding suggests that the hot corona emitting the bulk of the detected X-rays is elongated, rather than spherical. The X-ray polarization angle is consistent with that found in submillimeter wavelengths. Since the submillimeter polarization was found to be aligned with the jet direction in other X-ray binaries, this indicates that the corona is elongated orthogonal to the jet

X-Ray Polarized View of the Accretion Geometry in the X-Ray Binary Circinus X-1

Cir X-1 is a neutron star X-ray binary characterized by strong variations in flux during its eccentric ∼16.6 day orbit. There are also strong variations in the spectral state, and it has historically shown both atoll and Z state properties. We observed the source with the Imaging X-ray Polarimetry Explorer during two orbital segments, 6 days apart, for a total of 263 ks. We find an X-ray polarization degree in these segments of 1.6% ± 0.3% and 1.4% ± 0.3% at polarization angles of 37° ± 5° and −12° ± 7°, respectively. Thus, we observed a rotation of the polarization angle by 49° ± 8° along the orbit. Because variations of accretion flow, and then of the hardness ratio, are expected during the orbit, we also studied the polarization binned in hardness ratio and found the polarization angle differing by 67° ± 11° between the lowest and highest values of the hardness ratio. We discuss possible interpretations of this result that could indicate a possible misalignment between the symmetry axes of the accretion disk and the Comptonizing region caused by the misalignment of the neutron star’s angular momentum with respect to the orbital one

Polarization Properties of the Weakly Magnetized Neutron Star X-Ray Binary GS 1826-238 in the High Soft State

The launch of the Imaging X-ray Polarimetry Explorer (IXPE) on 2021 December 9 has opened a new window in X-ray astronomy. We report here the results of the first IXPE observation of a weakly magnetized neutron star, GS 1826−238, performed on 2022 March 29-31 when the source was in a high soft state. An upper limit (99.73% confidence level) of 1.3% for the linear polarization degree is obtained over the IXPE 2-8 keV energy range. Coordinated INTEGRAL and NICER observations were carried out simultaneously with IXPE. The spectral parameters obtained from the fits to the broadband spectrum were used as inputs for Monte Carlo simulations considering different possible geometries of the X-ray emitting region. Comparing the IXPE upper limit with these simulations, we can put constraints on the geometry and inclination angle of GS 1826-238

The detection of polarized X-ray emission from the magnetar 1E 2259+586

We report on IXPE, NICER, and XMM–Newton observations of the magnetar 1E 2259+586. We find that the source is significantly polarized at about or above 20 per cent for all phases except for the secondary peak where it is more weakly polarized. The polarization degree is strongest during the primary minimum which is also the phase where an absorption feature has been identified previously. The polarization angle of the photons are consistent with a rotating vector model with a mode switch between the primary minimum and the rest of the rotation of the neutron star. We propose a scenario in which the emission at the source is weakly polarized (as in a condensed surface) and, as the radiation passes through a plasma arch, resonant cyclotron scattering off of protons produces the observed polarized radiation. This confirms the magnetar nature of the source with a surface field greater than about 1015 G

The Polarized Cosmic Hand: IXPE Observations of PSR B1509−58/MSH 15−52

We describe IXPE polarization observations of the pulsar wind nebula (PWN) MSH 15−52, the “Cosmic Hand.” We find X-ray polarization across the PWN, with B-field vectors generally aligned with filamentary X-ray structures. High-significance polarization is seen in arcs surrounding the pulsar and toward the end of the “jet,” with polarization degree PD > 70%, thus approaching the maximum allowed synchrotron value. In contrast, the base of the jet has lower polarization, indicating a complex magnetic field at significant angle to the jet axis. We also detect significant polarization from PSR B1509−58 itself. Although only the central pulse phase bin of the pulse has high individual significance, flanking bins provide lower-significance detections and, in conjunction with the X-ray image and radio polarization, can be used to constrain rotating vector model solutions for the pulsar geometry