36 research outputs found
Callus induction and plant regeneration from broccoli (Brassica oleracea var. italica) for transformation
We evaluated the efficiency of callus induction and plantlet regeneration from hypocotyl explants of broccoli (Brassica oleracea var. italica). The cultivars were âMarathonâ, âGreenbeltâ, and âShogunâ. Transformation success was not affected by the presence of tobacco feeder-cell layers on the culture media. The frequency of shoot regeneration was greater from 10-d-old hypocotyls than from 14-d-old hypocotyls. Both âMarathonâ and âGreenbeltâ had higher potentials for tissue regeneration than did âShogunâ. We found that for transformation selection, the optimum concentration was either 50 mg/L kanamycin or 100 mg/L genetkin