The dynamic right-to-left translocation of Cerl2 is involved in the regulation and termination of nodal activity in the mouse node

The determination of left-right body asymmetry in mouse embryos depends on the interplay of molecules In a highly sensitive structure, the node. Here, we show that the localization of Cerl2 protein does not correlate to its mRNA expression pattern, from 3-somite stage onwards. Instead, Cerl2 protein displays a nodal flow-dependent dynamic behavior that controls the activity of Nodal in the node, and the transmission of the laterality information to the left lateral plate mesoderm (LPM). Our results indicate that Cerl2 initially localizes and prevents the activation of Nodal genetic circuitry on the right side of the embryo, and later its right-to-left translocation shutdowns Nodal activity in the node. The consequent prolonged Nodal activity in the node by the absence of Cerl2 affects local Nodal expression and prolongs its expression in the LPM. Simultaneous genetic removal of both Nodal node inhibitors, Cerl2 and Lefty1, sustains even longer and bilateral his LPM expression.F.C.T.; IBB/CBME, LAinfo:eu-repo/semantics/publishedVersio

Where and how machine learning plays a role in climate finance research

The financial sector, by mobilizing capital, is fundamental to adapt and mitigate the impact of climate change in the economy. This has led to the emergence of a new research field, climate finance, where experts are starting to harness Machine Learning (ML) as a tool to solve new problems, due to the need to use big datasets and to model complex non-linear relationships. We propose a review of the academic literature that goes beyond the existing bibliometric studies in the field, with the aim of identifying relevant application domains of this technology to inform ML experts where and how their modeling expertise may add value in climate finance. To achieve this, we first assemble a corpus of texts from three scientific databases and use Latent Dirichlet Allocation (LDA) for topic modeling, to uncover seven research areas which we label as: natural hazards, biodiversity, agricultural risk, carbon markets, energy economics, Environmental, Social and Governance (ESG) factors & investing, and climate data. Second, we perform an analysis of publication trends, which confirms that ML is growing both in breadth and depth in climate finance, in particular topics related to energy economics, ESG factors and climate data. Interestingly, some methods stand out in each area, based on data characteristics and modeling requirementsThe authors acknowledge financial help provided by grant PID2022-139614NB-C21 funded by MCIN/AEI/10.13039/501100011033/FEDER, E

Demonstration of negative refraction of microwaves

An experimental setup to demonstrate negative refraction is described. A simple method for designing and fabricating a metamaterial with negative refractive index at microwave frequencies is discussed. The metamaterial is made of a multilayer planar arrangement of flat unit cells. A prism was fabricated and used to demonstrate negative refraction at the prism-air interface. The prism is designed for demonstrations and works at the frequency of commercial microwave transmitters and receiver

Searching for new sources of innovative products for the food industry within halophyte aromatic plants: In vitro antioxidant activity and phenolic and mineral contents of infusions and decoctions of Crithmum maritimum L.

Aromatic halophyte plants are an outstanding source of bioactive compounds and natural products with potential use in the food industry. This work reports the in vitro antioxidant activity, toxicity, poly phenolic profile and mineral contents of infusions and decoctions from stems, leaves and flowers of Crithmum maritimum L, an aromatic and edible maritime halophyte (sea fennel). Aspalathus linearis (Burm.f.) Dahlg. (rooibos) herbal tea was used as a reference. Sea fennel's tisanes, particularly from leaves, were rich in phenolic compounds and five of them (p-hydroxybenzoic and ferulic acids, epicatechin, pyrocatechol and 4-hydroxybenzaldehyde) were here described in C maritimum for the first time. Chlorogenic acid was the dominant phenolic determined. Na was the most abundant mineral in all tisanes followed by Ca and Mg in leaves' tisanes and K in flowers. Sea fennel's samples had a similar antioxidant activity than those from A. linearis, and had no significant toxicity towards four different mammalian cell lines. Altogether, our results suggest that sea fennel can be a source of products and/or molecules for the food industry with antioxidant properties and minerals in the form, for example, of innovative health-promoting herbal beverages.FCT Investigator Programme [IF/00049/2012

application in C-H activation catalysis

This work was supported by the Spanish Government, the Spanish National Research Council (CSIC). We also thank Dr Martinez from Novozymes. Publisher Copyright: © 2023 RSC.The effect of the temperature in the synthesis of Pd nanoparticles in the metal-enzyme biohybrids is evaluated. The effect on the formation, size, and morphology of nanoparticles was evaluated using C. antarctica B lipase as the protein scaffold. XRD analyses confirmed the formation of crystalline Pd(0) as the metal species in all cases. TEM analyses revealed spherical crystalline nanoparticles with average diameter size from 2 nm at 4 °C synthesis to 10 nm obtained at 50 °C synthesis. The thermal phenomenon was also critical in the final hybrid formation using more complex enzymes, where the relation of the protein structure and temperature and the influence of the latter has been demonstrated to be critical in the reducing efficiency of the enzyme in the final Pd nanoparticle formation, in the metal species, or even in the final size of the nanoparticles. Different Pd biohybrids were evaluated as catalysts in the C-H activation of protected l-tryptophan under mild conditions. Pd@CALB4 showed the best results, with >99% conversion for C-2 arylation in methanol at room temperature with a TOF value of 64 min−1, being 2 or 4 times higher than that of the other synthesized hybrids. This catalyst showed a very high stability and recyclability, maintaining >95% activity after three cycles of use.publishersversionpublishe

Polymorphisms within Autophagy-Related Genes as Susceptibility Biomarkers for Multiple Myeloma: A Meta-Analysis of Three Large Cohorts and Functional Characterization

Autophagy; Genetic variants; Multiple myelomaAutofagia; Variantes genéticas; Mieloma múltipleAutofàgia; Variants genètiques; Mieloma múltipleMultiple myeloma (MM) arises following malignant proliferation of plasma cells in the bone marrow, that secrete high amounts of specific monoclonal immunoglobulins or light chains, resulting in the massive production of unfolded or misfolded proteins. Autophagy can have a dual role in tumorigenesis, by eliminating these abnormal proteins to avoid cancer development, but also ensuring MM cell survival and promoting resistance to treatments. To date no studies have determined the impact of genetic variation in autophagy-related genes on MM risk. We performed meta-analysis of germline genetic data on 234 autophagy-related genes from three independent study populations including 13,387 subjects of European ancestry (6863 MM patients and 6524 controls) and examined correlations of statistically significant single nucleotide polymorphisms (SNPs; p < 1 × 10−9) with immune responses in whole blood, peripheral blood mononuclear cells (PBMCs), and monocyte-derived macrophages (MDM) from a large population of healthy donors from the Human Functional Genomic Project (HFGP). We identified SNPs in six loci, CD46, IKBKE, PARK2, ULK4, ATG5, and CDKN2A associated with MM risk (p = 4.47 × 10−4−5.79 × 10−14). Mechanistically, we found that the ULK4rs6599175 SNP correlated with circulating concentrations of vitamin D3 (p = 4.0 × 10−4), whereas the IKBKErs17433804 SNP correlated with the number of transitional CD24+CD38+ B cells (p = 4.8 × 10−4) and circulating serum concentrations of Monocyte Chemoattractant Protein (MCP)-2 (p = 3.6 × 10−4). We also found that the CD46rs1142469 SNP correlated with numbers of CD19+ B cells, CD19+CD3− B cells, CD5+IgD− cells, IgM− cells, IgD−IgM− cells, and CD4−CD8− PBMCs (p = 4.9 × 10−4−8.6 × 10−4) and circulating concentrations of interleukin (IL)-20 (p = 0.00082). Finally, we observed that the CDKN2Ars2811710 SNP correlated with levels of CD4+EMCD45RO+CD27− cells (p = 9.3 × 10−4). These results suggest that genetic variants within these six loci influence MM risk through the modulation of specific subsets of immune cells, as well as vitamin D3−, MCP-2−, and IL20-dependent pathways.This work was supported by the European Union’s Horizon 2020 research and innovation program, N° 856620 and by grants from the Instituto de Salud Carlos III and FEDER (Madrid, Spain; PI17/02256 and PI20/01845), Consejería de Transformación Económica, Industria, Conocimiento y Universidades and FEDER (PY20/01282), from the CRIS foundation against cancer, from the Cancer Network of Excellence (RD12/10 Red de Cáncer), from the Dietmar Hopp Foundation and the German Ministry of Education and Science (BMBF: CLIOMMICS [01ZX1309]), and from National Cancer Institute of the National Institutes of Health under award numbers: R01CA186646, U01CA249955 (EEB). This work was also funded d by Portuguese National funds, through the Foundation for Science and Technology (FCT)—project UIDB/50026/2020 and UIDP/50026/2020 and by the project NORTE-01-0145-FEDER-000055, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF)

Sensorless Capacitor Voltage Balancing of a Grid-Tied, Single-Phase Hybrid Multilevel Converter with Asymmetric Capacitor Voltages using Dynamic Programming

This paper shows a sensorless capacitor voltage balancing control approach for a grid-connected, single-phase hybrid multilevel inverter based on an NPC main stage with a voltage stiff DC-link and an arbitrary number of H-Bridge modules (capacitor modules) with asymmetric capacitor voltages. Using nearest-level control, a model predictive control (MPC) approach with a prediction horizon of one time step is chosen to find an optimal switching-state combination among the redundant switching combinations to balance the capacitor voltages as quick as possible. Using the Lyapunov stability criterion, it is shown that an offline calculated optimal switching-state sequence for each discrete output voltage level can be used to operate the inverter without using any voltage sensors for the capacitor voltages. To validate the stability of the approach, a laboratory inverter with a resistive load is operated with the offline calculated optimal switching-state sequences and it is shown that the capacitor voltages converge to their desired reference voltages

Exploring Thermomyces lanuginosus Lipase (TLL)-PdNPs Nanohybrid as Suitable Catalyst for One-pot Synthesis of Bis(3-indolyl)phenylmethane

This work was supported by the Spanish Government, the Spanish National Research Council (CSIC). project PTDC/QUI‐QOR/0712/2020. We also thank Dr. Martinez from Novozymes. We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI) Publisher Copyright: © 2023 The Authors. ChemCatChem published by Wiley-VCH GmbH.Palladium nanohybrids were synthesized and applied to the one-pot synthesis of bis(3-indolyl)methanes by selective C−C bond reaction from benzyl alcohol and indole. A T. lanuginosus lipase-palladium nanoparticles hybrid (Pd@TLL) was synthesized, yielding PdNPs with an average diameter size of 5 nm. This heterogeneous catalyst was first tested in the selective oxidation of benzyl alcohol to benzaldehyde in different solvents. Then, the direct formation of bis(3-indolyl)methane, by in situ oxidation and C−C coupling, was successfully evaluated under different conditions, obtaining >99 % conversion at 80 °C in toluene, with a TOF value of 9 min−1 and 89 % in pure water, demonstrating the versatility of these biohybrids.publishersversionpublishe

Short-term tear film stability, optical quality and visual performance in two dual-focus contact lenses for myopia control with different optical designs

Purpose To assess and compare short-term visual and optical quality and tear film stability between two dual-focus (DF) prototype myopia control contact lenses (CLs) having different inner zone diameters. Methods Twenty-eight myopic subjects were included in this randomised, double-masked crossover study. Refraction, best-corrected visual acuity (VA) and tear film stability were measured at baseline (i.e., when uncorrected). Subjects were then binocularly fitted with the DF CLs, with only the sensorial dominant eye being assessed. Lenses were of the same material and had inner zone diameters of either 2.1 mm (S design) or 4.0 mm (M design). Visual and physical short-term lens comfort, over-refraction, best-corrected VA, stereopsis at 40 cm, best-corrected photopic and mesopic contrast sensitivity (CS), size and shape of light disturbance (LD), wavefront aberrations, subjective quality of vision (QoV Questionnaire) and tear film stability were measured for each lens. Results Both CL designs decreased tear film stability compared with baseline (p < 0.05). VA and photopic CS were within normal values for the subjects' age with each CL. When comparing lenses, the M design promoted better photopic CS for the 18 cycles per degree spatial frequency (p < 0.001) and better LD (p < 0.02). However, higher-order aberrations were improved with the S design (p = 0.02). No significant difference between the two CLs was found for QoV scores and tear film stability. Conclusions Both DF CLs provided acceptable visual performance under photopic conditions. The 4.0 mm inner zone gave better contrast sensitivity at high frequencies and lower light disturbance, while the 2.1 mm central diameter induced fewer higher-order aberrations for a 5 mm pupil diameter. Both CLs produced the same subjective visual short-term lens comfort.This work was supported by Atracció de Talent Scholarship UV-INV_PREDOC18F2-886420 awarded to José Vicente García-Marqués and by the Portuguese Foundation for Science and Technology (FCT) in the framework of projects PTDC/SAU-BEB/098391/2008, PTDC/FIS-OPT/0677/2014 and the FCT Strategic Funding UID/FIS/04650/2013. The authors would like to thank Cristian Talens-Estarelles for his contribution to the measurement of contact lenses. The authors thank Miguel Faria-Ribeiro for lens design and Precilens for contact lens manufacturing and donation

Modeling post-fire mortality in pure and mixed forest stands in Portugal—A forest planning-oriented model

Assessing impacts of management strategies may allow designing more resistant forests to wildfires. Planning-oriented models to predict the effect of stand structure and forest composition on mortality for supporting fire-smart management decisions, and allowing its inclusion in forest management optimization systems were developed. Post-fire mortality was modeled as a function of measurable forest inventory data and projections over time in 165 pure and 76 mixed forest stands in Portugal, collected by the 5th National Forest Inventory plots (NFI) plus other sample plots from ForFireS project, intercepted within 2006–2008 wildfire perimeters’ data. Presence and tree survival were obtained by examining 2450 trees from 16 species one year after the wildfire occurrence. A set of logistic regression models were developed under a three-stage modeling system: firstly multiple fixed-effects at stand-level that comprises a sub-model to predict mortality from wildfire; and another for the proportion of dead trees on stands killed by fire. At tree-level due to the nested structure of the data analyzed (trees within stands), a mixed-effect model was developed to estimate mortality among trees in a fire event. The results imply that the variation of tree mortality decreases when tree diameter at breast height increases. Moreover, the relative mortality increases with stand density, higher altitude and steeper slopes. In the same conditions, conifers are more prone to die than eucalyptus and broadleaves. Pure stands of broadleaves exhibit noticeably higher fire resistance than mixed stands of broadleaves and others species compositionThis research was supported by Project UID/AGR/00239/2013, PTDC/AGR-CFL/64146/2006 “Decision support tools for integrating fire and forest management planning” and project FIRE-ENGINE “Flexible Design of Forest Fire Management Systems” (MIT/FSE/0064/2009), both funded by the Portuguese Science Foundation (FCT), and contributes to the activities of the ALTERFOR Project “Alternative models and robust decision-making for future forest management”—H2020-ISIB-2015-2/grant agreement No. 67654, funded by European Union Seventh Framework Programme. This research has received also funding from the European Union’s H2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No. 691149 (SuFoRun). The authors would like to thank the Portuguese Science Foundation for funding the doctoral scholarships of Brigite Botequim (SFRH/ BD/44830/2008) and the Post Doc grant SFRH/BPD/96806/2013 of Susete Marques. Researcher Jordi Garcia-Gonzalo was supported by a “Ramon y Cajal” research contract from the MINECO (Ref. RYC-2013-14262) and has received funding from CERCA Programme / Generalitat de Catalunya. In addition, the authors wish to acknowledge the Portuguese Forest Service (ICNF) for supplying the perimeters of wildfires and NFI Databases and ForFireS Project for providing the inventory DatabasesS