Prediction of body fat in adolescents: validity of the methods relative fat mass, body adiposity index and body fat index

To verify the validity of anthropometric methods body adiposity index (BAI), relative fat mass (RFM) and body fat index (BFI) to estimate body fat percentage (%BF) in adolescents. Methods A cross-sectional study was carried out with 420 Brazilian adolescents aged 15–19 years, stratified by age (< 18 years, n = 356; ≥ 18 years, n = 64) and sex (boys, n = 216; girls, n = 204). The Anthropometric measurements height, body weight, hip circumference and waist circumference were collected to calculate the %BF by BAI, RFM, BFI methods. Subsequently, %BF was measured by dual emission X-ray absorptiometry (DXA), adopted as a reference method. In the statistical analysis of the data, the Pearson correlation test and the paired t test between %BF obtained by the equations and by the DXA were performed. The method validation criterion was that 68% of individuals should be within an acceptable error range of ± 3.5% of BF and Cohen's Kappa index ≥ 0.61. Additionally, the Bland–Altman graphical analysis was performed. Results All methods showed a high correlation with DXA. For the Kappa index, only the RFM reached the criterion in the total sample (0.67) and in the sample < 18 years (0.68). None of the methods reached the criterion of 68% of the sample within the error range of ± 3.5% of BF. Conclusion The BAI, RFM and BFI equations were not valid for predicting BF in the studied sample according to the criteria adopted regardless of sex or age.info:eu-repo/semantics/publishedVersio

IKKβ targeting reduces KRAS-induced lung cancer angiogenesis in vitro and in vivo: A potential anti-angiogenic therapeutic target

Objectives: The ability of tumor cells to drive angiogenesis is an important cancer hallmark that positively correlates with metastatic potential and poor prognosis. Therefore, targeting angiogenesis is a rational therapeutic approach and dissecting proangiogenic pathways is important, particularly for malignancies driven by oncogenic KRAS, which are widespread and lack effective targeted therapies. Based on published studies showing that oncogenic RAS promotes angiogenesis by upregulating the proangiogenic NF-κB target genes IL-8 and VEGF, that NF-κB activation by KRAS requires the IKKβ kinase, and that targeting IKKβ reduces KRAS-induced lung tumor growth in vivo, but has limited effects on cell growth in vitro, we hypothesized that IKKβ targeting would reduce lung tumor growth by inhibiting KRAS-induced angiogenesis. Materials and methods: To test this hypothesis, we targeted IKKβ in KRAS-mutant lung cancer cell lines either by siRNA-mediated transfection or by treatment with Compound A (CmpdA), a highly specific IKKβ inhibitor, and used in vitro and in vivo assays to evaluate angiogenesis. Results and conclusions: Both pharmacological and siRNA-mediated IKKβ targeting in lung cells reduced expression and secretion of NF-κB-regulated proangiogenic factors IL-8 and VEGF. Moreover, conditioned media from IKKβ-targeted lung cells reduced human umbilical vein endothelial cell (HUVEC) migration, invasion and tube formation in vitro. Furthermore, siRNA-mediated IKKβ inhibition reduced xenograft tumor growth and vascularity in vivo. Finally, IKKβ inhibition also affects endothelial cell function in a cancer-independent manner, as IKKβ inhibition reduced pathological retinal angiogenesis in a mouse model of oxygen-induced retinopathy. Taken together, these results provide a novel mechanistic understanding of how the IKKβ pathway affects human lung tumorigenesis, indicating that IKKβ promotes KRAS-induced angiogenesis both by cancer cell-intrinsic and cancer cell-independent mechanisms, which strongly suggests IKKβ inhibition as a promising antiangiogenic approach to be explored for KRAS-induced lung cancer therapy

Embryonic dormancy in seeds of Bactris gasipaes Kunth (peach-palm)

Bactris gasipaes is a domesticated palm whose fruits are of great importance for the Amazonian people and whose heart of palm is also receiving economic interest in other brazilian and Latin America regions. The aim of this study was verify embryonic dormancy and its correlation with first cataphyll emergence in B. gasipaes seeds collected from four plants at Manaus city and four others at Coari city, both in the Amazonas state, Brazil. After extraction and cleaning, some of the seeds (4 replications of 25 per plant) were sown in a seedbed with a sawdust and sand mixture as substrate, and embryos (4 replications of 10 per plant), after extraction, were inoculated into half strength Murashige and Skoog cultures. Were used 100 seeds and 40 embryo per treatment. Whole seed and embryo germination varied between the different source plants and locations, with the greatest difference observed for the emergence of first cataphyll from seeds in the seedbed. For the most part of variables, results of seed and embryo were positively associated, namely, as one went up the other also, and vice versa. These results suggesting that, at least in part, seed dormancy in Bactris gasipaes is associated with embryonic dormancy. © 2017, Associacao Brasileira de Tecnologia de Sementes. All rights reserved

<i>Gaia</i> Data Release 1. Summary of the astrometric, photometric, and survey properties

Context. At about 1000 days after the launch of Gaia we present the first Gaia data release, Gaia DR1, consisting of astrometry and photometry for over 1 billion sources brighter than magnitude 20.7. Aims. A summary of Gaia DR1 is presented along with illustrations of the scientific quality of the data, followed by a discussion of the limitations due to the preliminary nature of this release. Methods. The raw data collected by Gaia during the first 14 months of the mission have been processed by the Gaia Data Processing and Analysis Consortium (DPAC) and turned into an astrometric and photometric catalogue. Results. Gaia DR1 consists of three components: a primary astrometric data set which contains the positions, parallaxes, and mean proper motions for about 2 million of the brightest stars in common with the HIPPARCOS and Tycho-2 catalogues – a realisation of the Tycho-Gaia Astrometric Solution (TGAS) – and a secondary astrometric data set containing the positions for an additional 1.1 billion sources. The second component is the photometric data set, consisting of mean G-band magnitudes for all sources. The G-band light curves and the characteristics of ∼3000 Cepheid and RR-Lyrae stars, observed at high cadence around the south ecliptic pole, form the third component. For the primary astrometric data set the typical uncertainty is about 0.3 mas for the positions and parallaxes, and about 1 mas yr−1 for the proper motions. A systematic component of ∼0.3 mas should be added to the parallax uncertainties. For the subset of ∼94 000 HIPPARCOS stars in the primary data set, the proper motions are much more precise at about 0.06 mas yr−1. For the secondary astrometric data set, the typical uncertainty of the positions is ∼10 mas. The median uncertainties on the mean G-band magnitudes range from the mmag level to ∼0.03 mag over the magnitude range 5 to 20.7. Conclusions. Gaia DR1 is an important milestone ahead of the next Gaia data release, which will feature five-parameter astrometry for all sources. Extensive validation shows that Gaia DR1 represents a major advance in the mapping of the heavens and the availability of basic stellar data that underpin observational astrophysics. Nevertheless, the very preliminary nature of this first Gaia data release does lead to a number of important limitations to the data quality which should be carefully considered before drawing conclusions from the data

Vegetative development, fruits yield and optimization of pineapple cv. Pérola with different levels of irrigation

Apesar de ser uma planta com necessidades hídricas relativamente baixas, o abacaxizeiro tem demanda permanente de água, variável ao longo do ciclo e dependente do seu estádio de desenvolvimento. Assim, objetivou-se analisar volumes de irrigação no desenvolvimento vegetativo, no rendimento da fruta e na otimização do abacaxizeiro cv. Pérola. O experimento foi realizado na Universidade Federal de Sergipe, município de São Cristóvão (11°01'S, 37°12'W), no delineamento em blocos ao acaso, com quatro tratamentos (lâminas de água) (100% da evaporação do tanque Classe A (523,7mm ano-1); 75% da evaporação do tanque Classe A (392,8mm ano-1), 50% da evaporação do tanque Classe A (261,8mm ano-1) e 0% da evaporação do tanque Classe A, seis repetições e 12 plantas úteis por parcela. O sistema de irrigação foi por aspersão convencional disposto em linha, com pressão de 20mca e vazão de 1,33m3 h-1. A área foliar (cm2) mínima atingida de 4552,6cm2 foi observada no volume de água de 122,9mm ano-1, enquanto a massa seca das folhas (147,6g) foi constatada com 17mm ano-1. O máximo comprimento da folha D (88,9cm) foi estimado com 532,7 mm ano-1. Já o máximo comprimento do fruto (23cm) foi observado na lâmina de 296,9mm ano-1. A massa do fruto máxima estimado de 1.736g foi constatado na lâmina de 356,4mm ano-1. No contexto, a irrigação contribui de forma positiva no desenvolvimento vegetativo e rendimento da fruta do abacaxizeiro. Ressalta-se remuneração mensal líquida de R 1.161,17ha-1, quando se adota lâmina de irrigação de 356,4mm ano-1. _________________________________________________________________________________________ ABSTRACT: Regardless a relatively low water needs, pineapple fruit has a variable behavior related to water need throughout its biological cycle depending on the development stage. The objective of this work was to analyze irrigation on the vegetative development and fruits yield in pineapple cv. Pérola. The experiment was conducted at the Universidade Federal de Sergipe Experimental Station, at São Cristóvão - SE (11°01'S, 37°12'W), in a randomized block design, with four water level treatments as follow: 100% of Class-A (523.7mm ano-1) evaporation pan; 75% of Class-A (392.8mm ano-1) evaporation pan, 50% of Class-A (261.8mm ano-1) evaporation pan and 0% of Class-A evaporation pan, in six replications and twelve plants per plot. The splinkler watering system of irrigation was installed in a line, with an operating pression of 20 mca and a sprinkler dischrage of 1.33m3h-1. A minimum leaf area of 4552.6cm2 was observed, considering a water level of 122.9mm year-1, while a leaf dry matter was 147.6 g in 17mmyear-1. The maximum leaf length (D) of 88.9cm was estimated considering 532.7mmyear-1. The maximum fruit length of 23cm was observed, considering a water level of 296.9mmyear-1. The maximum estimated fruit weight was 1.736g in a water level of 356.4mm year-1. The irrigation seems to contribute in a positive way to the vegetative development in the pineapple fruit yield. It is important to mention that it could be observed a net profit of R 1,161.17 ha-1, when a water level of 356.4mm ano-1 of irrigation was adopted