Study of angular cutting conditions using multiple scratch tests onto low carbon steel: An experimental-numerical approach

Multiple parallel scratches are often analyzed to understand the material removal mechanisms due to abrasion. However, successive scratches with different orientations may represent better the conditions found in machining processes, such as honing and belt finishing. The objective of this work was to analyze the cutting forces and the phenomena of material removal due to abrasion, arising from angular scratches in low carbon steel. Experimental and numerical techniques were considered. In both, analyses considered the presence of an initial set of parallel scratches, followed by a second set of scratches with different orientations (10, 20 or 30°) with respect to the previous one. The cutting action was performed by a tool representing an abrasive particle, which had a cono-spherical geometry with 235μm tip radius and 30° apex angle. The cutting settings were: 50m/min scratch velocity and 100μm depth of cut. In the experimental part, scratches were conducted using a shaper machine tool equipped with a tungsten carbide (WC-Co) stylus. Tests were conducted on a Kistler platform, which allowed force measurement. Surfaces were later analyzed with an optical profilometer. The numerical simulations considered a ductile damage model with element deletion to provide the material removal during the scratches. Experimental and numerical results showed that the angle affects the cutting forces, especially when one scratch crosses a previously scratched region. The 20° case was the most critical, especially in terms of the cutting forces, due to the accentuated material strain-hardening for this condition. Likewise, this fact was corroborated by numerical results, which indicated a higher energy necessary to plastic deformation, and a reduced material removal at 20°

Tracing the Distribution of European Lactase Persistence Genotypes Along the Americas

In adulthood, the ability to digest lactose, the main sugar present in milk of mammals, is a phenotype (lactase persistence) observed in historically herder populations, mainly Northern Europeans, Eastern Africans, and Middle Eastern nomads. As the –13910∗T allele in the MCM6 gene is the most well-characterized allele responsible for the lactase persistence phenotype, the –13910C > T (rs4988235) polymorphism is commonly evaluated in lactase persistence studies. Lactase non-persistent adults may develop symptoms of lactose intolerance when consuming dairy products. In the Americas, there is no evidence of the consumption of these products until the arrival of Europeans. However, several American countries’ dietary guidelines recommend consuming dairy for adequate human nutrition and health promotion. Considering the extensive use of dairy and the complex ancestry of Pan-American admixed populations, we studied the distribution of –13910C > T lactase persistence genotypes and its flanking haplotypes of European origin in 7,428 individuals from several Pan-American admixed populations. We found that the –13910∗T allele frequency in Pan-American admixed populations is directly correlated with allele frequency of the European sources. Moreover, we did not observe any overrepresentation of European haplotypes in the –13910C > T flanking region, suggesting no selective pressure after admixture in the Americas. Finally, considering the dominant effect of the –13910∗T allele, our results indicate that Pan-American admixed populations are likely to have higher frequency of lactose intolerance, suggesting that general dietary guidelines deserve further evaluation across the continent