Wound repair and regeneration.

The repair of wounds is one of the most complex biological processes that occur during human life. After an injury, multiple biological pathways immediately become activated and are synchronized to respond. In human adults, the wound repair process commonly leads to a non-functioning mass of fibrotic tissue known as a scar. By contrast, early in gestation, injured fetal tissues can be completely recreated, without fibrosis, in a process resembling regeneration. Some organisms, however, retain the ability to regenerate tissue throughout adult life. Knowledge gained from studying such organisms might help to unlock latent regenerative pathways in humans, which would change medical practice as much as the introduction of antibiotics did in the twentieth century

From scarless fetal wounds to keloids: Molecular studies in wound healing

10.1046/j.1524-475X.2003.11604.xWound Repair and Regeneration116411-418WRER

Differential Transcriptional Responses of Keloid and Normal Keratinocytes to Serum Stimulation

10.1016/j.jss.2006.01.031Journal of Surgical Research1351156-163JSGR

Investigation of the influence of keloid-derived keratinocytes on fibroblast growth and proliferation in vitro

Plastic and Reconstructive Surgery1073797-808PRSU

A comparative analysis of the osteogenic effects of BMP-2, FGF-2, and VEGFA in a calvarial defect model.

The utilization of growth factors for bone regeneration is a widely studied field. Since the approval of bone morphogenetic protein-2 (BMP-2) for therapeutic use in humans, the concept of utilizing growth factors for bone regeneration in translational medicine has become even more attractive. Despite many studies published on individual growth factors in various bone models, comparative analysis is largely lacking. The aim of our study was to compare three different proosteogenic factors under identical in vivo conditions. Thus, we tested the bone regeneration capacity of the three different growth factors BMP-2, fibroblast growth factor-2 (FGF-2), and vascular endothelial growth factor A (VEGFA) in a calvarial defect model. We demonstrated that BMP-2 and VEGFA had similar bone healing capacities, resulting in complete calvarial healing as early as week 3. FGF-2 also showed a significantly higher bone regeneration capacity; however, the healing rate was lower than with BMP-2 and VEGFA. Interestingly, these findings were paralleled by an increased angiogenic response upon healing in BMP-2– and VEGFA-treated calvarial defects as compared with FGF-2. Immunohistochemistry for proliferating and osteoprogenitor cells revealed activity at different points after surgery among the groups. In conclusion, we demonstrated an efficient bone regeneration capacity of both BMP-2 and VEGFA, which was superior to FGF-2. Moreover, this study highlights the efficient bone regeneration of VEGFA, which was comparable with BMP-2. These data provide a valuable comparative analysis, which can be used to further optimize growth factor–based strategies in skeletal tissue engineering

Increased transcriptional response to mechanical strain in keloid fibroblasts due to increased focal adhesion complex formation

10.1002/jcp.20486Journal of Cellular Physiology2062510-517JCLL

Increased CCN2 transcription in keloid fibroblasts requires cooperativity between AP-1 and SMAD binding sites

10.1097/SLA.0b013e318070d54fAnnals of Surgery2465886-895ANSU