10 research outputs found
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Transcriptional Profiling Using the Flowthrough Genosensor
A Cooperative Research and Development Agreement (CRADA) between Lockheed Martin Energy Research Corporation (Contractor) and Gene Logic, Inc., (Participant) was carried out to evaluate the technical feasibility study of the application of the flowthrough genosensor for gene expression (transcriptional) profiling, over the current industry practice of using flat surface hybridization arrays to monitor the relative abundance of individual mRNA species in a cell. Various parameters, including substrate preparation, flow rates, hybridization conditions and sample concentrations, were evaluated on the flowthrough genosensor. The superiority of the flowthrough genosensor, in terms of hybridization rate and sensitivity were established
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Dual Manifold System for Arraying Biomolecules
The objective of this CRADA is to establish a new approach to fluid transfer and array construction. This new approach will involve a high-speed, multiplexed fluid distribution valve and ink jet valves. It will enable the parallel handling of multiple reagents for a system that will have multiple applications in addition to the high-speed construction of microarrays. The primary tasks involve proof of principle experiments aimed at establishing key components of the technology and evaluating various optional configurations. The basic platform for evaluating the technology will be set-up by the Contractor at Oak Ridge National Laboratory (ORNL) and will employ custom valving prepared by Rheodyne. The test platform will consist of a motion controller, 3-axes of motion, software, and pneumatic control; and will be used to evaluate the hybrid valve
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CRADA Final Report-Dual Manifold System for Arraying Biomolecules
The objective of this CRADA is to establish a new approach to fluid transfer and array construction. This new approach will involve a high-speed, multiplexed fluid distribution valve and ink jet valves. It will enable the parallel handling of multiple reagents for a system that will have multiple applications in addition to the high-speed construction of microarrays. The primary tasks involve proof of principle experiments aimed at establishing key components of the technology and evaluating various optional configurations. The basic platform for evaluating the technology will be set-up by the Contractor at Oak Ridge National Laboratory (ORNL) and will employ custom valving prepared by Rheodyne. The test platform will consist of a motion controller, 3-axes of motion, software, and pneumatic control; and will be used to evaluate the hybrid valve
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Using Allele-Specific PCR with Molecular Beams as a Means for Genotyping the Diallelic Indels
The first Specific Aim for this grant was to identify and characterize an average of 500 human insertion/deletion polymorphisms per grant year (1500 total). This task was carried out entirely at MMRF. They substantially exceeded this goal by confirming about 2,300 diallelic indels. Complete characterization information for these polymorphisms is available from the Marshfield web site. A manuscript describing results for the first 2,000 diallelic indels was published earlier this year in the American Journal of Human Genetics. The Second Specific Aim of the grant was to investigate and develop improved methods for analysis of diallelic polymorphisms using miniaturized DNA arrays. The initial genotyping technology efforts focused on various hybridization and extension protocols with oligo arrays on flow-through channel glass. Channel glass is a porous material that permits reagents to be passed through the arrays. They devoted roughly 19 months at the beginning of the grant in pursuit of this methodology, but for various technological reasons, progress was limited