71 research outputs found
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A simple computer program for quantitation and scatchard analysis of steroid receptor proteins
This paper presents a relatively simple program for the analysis of data in tissue receptor protein assays. Information is presented to the computer as c.p.m. obtained from liquid scintillation systems. The program is adaptable to clinical studies while retaining sufficient flexibility to deal with cross-competition data and the generation of regression lines for Scatchard plot analyses
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270. Isolation and characterization of stable clonal lines of HTC (Hepatoma) cells, non-inducible for tyrosine aminotransferase by glucocorticoids
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Retinoids and cultured human fibroblasts: Effects on cell growth and presence of cellular retinoic acid-binding protein
We have examined the effects of retinoids on growth of cultured human skin fibroblasts from four individuals. Retinoic acid and retinol both produce a dose-dependent inhibition of growth in the four strains examined; retinoic acid was more potent than retinol in this respect. The growth inhibitory effect of retinoic acid is characterized by a decrease in the exponential growth rate, which is reversible upon removal of retinoic acid from the growth medium; the final saturation density, however, is not modified by retinoic acid treatment. No alterations of cell morphology, viability, or adhesiveness to substratum are induced by the retinoid concentrations utilized. The inhibitory effect of 10
−6 M retinoic acid on cell growth is not affected by the concentration of fetal calf serum (FCS) in the medium. In all four human fibroblast strains examined, specific binding of [
3H]retinoic acid to cytosol is present as determined by sucrose-density gradient centrifugation. Despite the effects of retinol on fibroblast growth, no cytoplasmic binding of [
3H]retinol could be demonstrated in these cells
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