An efficient method for DNA extraction from Cladosporioid fungi

We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells. In order to overcome this we grew these fungi for three days on agar plates and extracted DNA from mycelium mats after manual or electric homogenization. High-quality DNA was isolated, with an A260/A280 ratio ranging between 1.6 and 2.0. Isolated genomic DNA was efficiently digested with restriction enzymes and produced distinct banding patterns on agarose gels for the different Cladosporium species. Clear DNA fragments from the isolated DNA were amplified by PCR using small and large subunit rDNA primers, demonstrating that this method provides DNA of sufficiently high quality for molecular analyse

Modelling Entrepreneurship

Two approaches can be distinguished with respect to modelling entrepreneurship: (i) the approach focusing on the net development of the number of entrepreneurs in an equilibrium framework and (ii) the approach focusing on the entries and exits of entrepreneurs. In this paper we unify these ap- proaches to arrive at a model explaining the equilibrium and actual number of entrepreneurs and the entry and exit rate of entrepreneurs simultaneously and consistently. We apply our unified approach to the Netherlands using self-employment data for the 1960-99 period. We find error-correction of about 20% per year and a very different situation in terms of disequilibrium before and after the early 1980's. Periods of high unemployment appear to be characterized by both high entry and high exit rates

Gene expression patterns in anterior pituitary associated with quantitative measure of oestrous behaviour in dairy cows

Intensive selection for high milk yield in dairy cows has raised production levels substantially but at the cost of reduced fertility, which manifests in different ways including reduced expression of oestrous behaviour. The genomic regulation of oestrous behaviour in bovines remains largely unknown. Here, we aimed to identify and study those genes that were associated with oestrous behaviour among genes expressed in the bovine anterior pituitary either at the start of oestrous cycle or at the mid-cycle (around day 12 of cycle), or regardless of the phase of cycle. Oestrous behaviour was recorded in each of 28 primiparous cows from 30 days in milk onwards till the day of their sacrifice (between 77 and 139 days in milk) and quantified as heat scores. An average heat score value was calculated for each cow from heat scores observed during consecutive oestrous cycles excluding the cycle on the day of sacrifice. A microarray experiment was designed to measure gene expression in the anterior pituitary of these cows, 14 of which were sacrificed at the start of oestrous cycle (day 0) and 14 around day 12 of cycle (day 12). Gene expression was modelled as a function of the orthogonally transformed average heat score values using a Bayesian hierarchical mixed model on data from day 0 cows alone (analysis 1), day 12 cows alone (analysis 2) and the combined data from day 0 and day 12 cows (analysis 3). Genes whose expression patterns showed significant linear or non-linear relationships with average heat scores were identified in all three analyses (177, 142 and 118 genes, respectively). Gene ontology terms enriched among genes identified in analysis 1 revealed processes associated with expression of oestrous behaviour whereas the terms enriched among genes identified in analysis 2 and 3 were general processes which may facilitate proper expression of oestrous behaviour at the subsequent oestrus. Studying these genes will help to improve our understanding of the genomic regulation of oestrous behaviour, ultimately leading to better management strategies and tools to improve or monitor reproductive performance in bovines

Geometric Second Order Field Equations for General Tensor Gauge Fields

Higher spin tensor gauge fields have natural gauge-invariant field equations written in terms of generalised curvatures, but these are typically of higher than second order in derivatives. We construct geometric second order field equations and actions for general higher spin boson fields, and first order ones for fermions, which are non-local but which become local on gauge-fixing, or on introducing auxiliary fields. This generalises the results of Francia and Sagnotti to all representations of the Lorentz group.Comment: 34 pages, LaTeX. Reference adde

Higher-Spin Matter Interactions in 2+1 Dimensions

We describe a model of massive matter fields interacting through higher-spin gauge fields in 2+1 dimensional space-time. The two main conclusions are that the parameter of mass $M$ appears as a module characterizing an appropriate vacuum solution of the full non-linear model and that $M$ affects a structure of a global vacuum higher-spin symmetry which leaves invariant the chosen vacuum solution.Comment: LaTex, 9 pages, no figures, Invited talk at the 10 International Conference Problems of Quantum Field Theory, Alushta (Cremea - Ukrain), May 13-18, 199

Superfield Theories in Tensorial Superspaces and the Dynamics of Higher Spin Fields

We present the superfield generalization of free higher spin equations in tensorial superspaces and analyze tensorial supergravities with GL(n) and SL(n) holonomy as a possible framework for the construction of a non-linear higher spin field theory. Surprisingly enough, we find that the most general solution of the supergravity constraints is given by a class of superconformally flat and OSp(1|n)-related geometries. Because of the conformal symmetry of the supergravity constraints and of the higher spin field equations such geometries turn out to be trivial in the sense that they cannot generate a `minimal' coupling of higher spin fields to their potentials even in curved backgrounds with a non-zero cosmological constant. This suggests that the construction of interacting higher spin theories in this framework might require an extension of the tensorial superspace with additional coordinates such as twistor-like spinor variables which are used to construct the OSp(1|2n) invariant (`preonic') superparticle action in tensorial superspace.Comment: LaTeX, 30 pages, no figures. V2. Discussion on conventional constraints extended, typos corrected, JHEP style, to appear in JHE

Higher-Spin Gauge Interactions for Massive Matter Fields in 3D AdS Space-Time

A remarkable feature of the models with interactions exhibiting higher-spin (HS) gauge symmetries in $d>2$ is that their most symmetric vacua require (anti)-de Sitter (AdS) geometry rather than the flat one. In striking parallelism to what might be expected of M theory HS gauge theories describe infinite towers of fields of all spins and possess naturally space-time SUSY and Chan-Paton type inner symmetries. In this paper, we analyze at the level of the equations of motion the simplest non-trivial HS model which describes HS gauge interactions (on the top of the usual supergravitational and (Chern-Simons) Yang-Mills interactions) of massive spin-0 and spin-1/2 matter fields in d=2+1 AdS space-time. The parameter of mass of the matter fields is identified with the vev of a certain auxiliary field in the model. The matter fields are shown to be arranged into d3 N=2 massive hypermultiplets in certain representations of $U(n)\times U(m)$ Yang-Mills gauge groups. Discrete symmetries of the full system are studied, and the related N=1 supersymmetric truncations with O(n) and Sp(n) Yang-Mills symmetries are constructed. The simplicity of the model allows us to elucidate some general properties of the HS models. In particular, a new result, which can have interesting implications to the higher-dimensional models, is that our model is shown to admit an "integrating" flow that proves existence of a non-local B\"acklund-Nicolai-type mapping to the free system.Comment: LaTeX, 46 pages, no figures; minor corrections, typo

Exploring Outcomes to Consider in Economic Evaluations of Health Promotion Programs: What Broader Non-Health Outcomes Matter Most?

Background Attention is increasing on the consideration of broader non-health outcomes in economic evaluations. It is unknown which non-health outcomes are valued as most relevant in the context of health promotion. The present study fills this gap by investigating the relative importance of non-health outcomes in a health promotion context. Method We investigated the relative importance of ten non-health outcomes of health promotion programs not commonly captured in QALYs. Preferences were elicited from a sample of the Dutch general public (N = 549) by means of a ranking task. These preferences were analyzed using Borda scores and rank-ordered logit models. Results The relative order of preference (from most to least important) was: self-confidence, insights into own (un)healthy behavior, perceived life control, knowledge about a certain health problem, social support, relaxation, better educational achievements, increased labor participation and work productivity, social participation, and a reduction in criminal behavior. The weight given to a particular non-health outcome was affected by the demographic variables age, gender, income, and education. Furthermore, in an open question, respondents mentioned a number of other relevant non-health outcomes, which we classified into outcomes relevant for the individual, the direct social environment, and for society as a whole. Conclusion The study provides valuable insights in the non-health outcomes that are considered as most important by the Dutch general population. Ideally, researchers should include the most important non-health outcomes in economic evaluations of health promotio

Nonlinear Equations for Symmetric Massless Higher Spin Fields in (A)dSd(A)dS_d

Nonlinear field equations for totally symmetric bosonic massless fields of all spins in any dimension are presented.Comment: 19 pages, latex, no figures, v2 minor correction