RAZVOJ METODE BRZE MIKROPROPAGACIJE ALOJE VERE (Aloa vera L.)

An efficient micropropagation method has been developed using shoot tip explants in Aloe vera L. The process involves subsequent in vitro morphogenesis and rooting of the in vitro proliferated shoots and transplantation of regenerated plants under ex vitro condition. Shoot proliferation was found best in MS medium containing BA 2.0 mg/l, KIN 0.5 mg/l and NAA 0.2 mg/l. Maximum 98.96% shoots were proliferated in this media composition. This media composition is best comparing to other treatment used in this study. Highest shoot number per explant was also achieved in the same medium within 5 weeks. In case of adventitious rooting, MS medium containing NAA 0.2 mg/l and NAA 0.5 mg/l was found to be the best. Maximum 80.25% rooting and highest number of root per culture (6.71) was obtained in this media composition. After transplanting the 20 days old rooted shoots into mixture of garden soil, compost and sand (2:1:1), 80% of survivability after 5 weeks was an achieved. Regenerated plants after acclimatization were transferred to soil and they showed 82% survival. The regenerated plants were morphologically similar to the mother plants.Razvijena je djelotvorna metoda mikrorazmnožavanja aloje vere (Aloa vera L.) upotrebom vršnih izboja biljke. Postupak uključuje sljedeću in vitro morfogenezu i ukorjenje in vitro razmnoženih izboja te transplataciju regeneriranih biljaka u ex vitro uvjetima. Razmnažanje se pokazalo najbolje u MS supstratu koji je sadržavao BA 2,0 mg/l, KIN 0,5 mg/l i NAA 02 mg/l. Razmnožen je maksimum od 98,96% izboja u tom sastavu supstrata. Najveći broj izboja po eksplantu također je postignut u istom supstratu u 5 tjedana. Kod adventivnog ukorjenjavanja najboljim se pokazao MS supstrat koji je sadržavao NAA 0,2 mg/l + NAA 0,5 mg/l. Maksimum od 80,25% ukorjenjenja i najveći broj korijena po eksplantu (6,71) dobiven je u supstratu istog sastava. Nakon presađivanja 20 dana starih ukorijenjenih izboja u mješavinu vrtnog tla, komposta i pijeska (2:1:1) postignuto je 80% preživljavanja nakon 5 tjedana. Regenerirane biljke su nakon aklimatiziranja presađene u zemlju uz preživljavanje od 82%. Regenerirane biljke bile su morfološki slične matičnim biljkama