PrzeciweozynofilowedziałanieIL-12 w hodowanej tkance ludzkiego polipa nosa

Rationale: Tissue eosinophilia is an important pathophysiological issue in bronchial ashma and nasal polyps. Its magnitude is regulated by several mechanisms, including selective migration of eossinophils to peripheral tissues and prolongation of survival. A key role in this processes is assigned to Th2 originating cytokines: IL-3, IL-5, GM-CSF. It has been previously demonstrated that IL-12 diminishes tissue eosinophilia in an animal model, and Hofstra showed that Il-12 together with IL-18 prevents allergen-induced increase bronchial hyperresponsiveness, BAL eosinophilia and the development of allergen-specific Th2 cells. Methods: Nasal polyps were obtained during routine surgery and were cultured in fragments of approximately 30 mg for 2, 6, and 15 days in RPMI 1640 in the absence or presence of IL-12. Afterwards a dose-dependency was tested at day 2 of culture. Polyp tissue from cultures was than processed to slides, stained with Giemsa and cells were counted in light microscopy (400x). Results: Eosinophils represented 62,8 &plusmn; 21,3% of residing cells in nasal polyps at the day 0. IL-12 (1 &#956;g/ml) caused a significant time-dependent decrease in the percentage of Eos after 2 and 6 days. The effect of IL-12 at day 2 was concentration-dependent: control, 28,2 &plusmn; 2,9; at 10 ng/ml, 13,9 &plusmn; 6,4 (n = 4, p < 0,05); at 100 ng/ml, 11,6 &plusmn; 2,1 (p < 0.01); at 1 &#956;g/ml, 7,5 &plusmn; 1,5 (p < 0,005). Conclusion: IL-12 acts as potent topical antieosinophilic agent. Its action can be seen in a cultured polyp environment. It is visible already after two days and is concentration-dependent. Further study is needed to elucidate tissue mechanisms of this action

Critical ischemia of the fingers in an auto mechanic as a result of occupational exposure

Hypothenar hammer syndrome is a rare cause of ischemic fingers observed mainly in young men smoking cigarettes and it is associated with repeated trauma of the ulnar artery in the area of the hypothenar eminence of the dominant-hand arm, resulting in a deficit of blood supply with the occurrence of hand symptoms typical for chronic and sometimes critical ischemia. Artery injury in this location is most often the result of multiple repetitions of the same activity being mostly the result of occupational exposure. We present a case of a 27-year-old car mechanic admitted to the hospital with symptoms of critical ischemia of the fingers III, IV, and V of the right hand, which resolved after conservative treatment