Connexin 40 promoter-based enrichment of embryonic stem cell-derived cardiovascular progenitor cells

Background: Pluripotent embryonic stem (ES) cells that can differentiate into functional cardiomyocytes as well as vascular cells in cell culture may open the door to cardiovascular cell transplantation. However, the percentage of ES cells in embryoid bodies (EBs) which spontaneously undergo cardiovascular differentiation is low (< 10%), making strategies for their specific labeling and purification indispensable. Methods: The human connexin 40 (Cx40) promoter was isolated and cloned in the vector pEGFP. The specificity of the construct was initially assessed in Xenopus embryos injected with Cx40-EGFP plasmid DNA. Stable Cx40-EGFP ES cell clones were differentiated and fluorescent cells were enriched manually as well as via fluorescence-activated cell sorting. Characterization of these cells was performed with respect to spontaneous beating as well as via RT-PCRs and immunofluorescent stainings. Results: Cx40-EGFP reporter plasmid injection led to EGFP fluorescence specifically in the abdominal aorta of frog tadpoles. After crude manual enrichment of highly Cx40-EGFP- positive EBs, the appearance of cardiac and vascular structures was increased approximately 3-fold. Immuno fluorescent stainings showed EGFP expression exclusively in vascular-like structures simultaneously expressing von Willebrand factor and in formerly beating areas expressing alpha-actinin. Cx40-EGFP-expressing EBs revealed significantly higher numbers of beating cardiomyocytes and vascular-like structures. Semiquantitative RT-PCRs confirmed an enhanced cardiovascular differentiation as shown for the cardiac markers Nkx2.5 and MLC2v, as well as the endothelial marker vascular endothelial cadherin. Conclusions: Our work shows the feasibility of specific labeling and purification of cardiovascular progenitor cells from differentiating EBs based on the Cx40 promoter. We provide proof of principle that the deleted CD4 (Delta CD4) surface marker-based method for magnetic cell sorting developed by our group will be ideally suitable for transference to this promoter. Copyright (c) 2008 S. Karger AG, Basel

Replica bounds for diluted non-Poissonian spin systems

In this paper we extend replica bounds and free energy subadditivity arguments to diluted spin-glass models on graphs with arbitrary, non-Poissonian degree distribution. The new difficulties specific of this case are overcome introducing an interpolation procedure that stresses the relation between interpolation methods and the cavity method. As a byproduct we obtain self-averaging identities that generalize the Ghirlanda-Guerra ones to the multi-overlap case.Comment: Latex file, 15 pages, 2 eps figures; Weak point revised and corrected; Misprints correcte

Penumbral thermal structure below the visible surface

$Context$. The thermal structure of the penumbra below its visible surface (i.e., $\tau_5 \ge 1$) has important implications for our present understanding of sunspots and their penumbrae: their brightness and energy transport, mode conversion of magneto-acoustic waves, sunspot seismology, and so forth. $Aims$. We aim at determining the thermal stratification in the layers immediately beneath the visible surface of the penumbra: $\tau_5 \in [1,3]$ ($\approx 70-80$ km below the visible continuum-forming layer). $Methods$. We analyzed spectropolarimetric data (i.e., Stokes profiles) in three Fe \textsc{i} lines located at 1565 nm observed with the GRIS instrument attached to the 1.5-meter solar telescope GREGOR. The data are corrected for the smearing effects of wide-angle scattered light and then subjected to an inversion code for the radiative transfer equation in order to retrieve, among others, the temperature as a function of optical depth $T(\tau_5)$. $Results$. We find that the temperature gradient below the visible surface of the penumbra is smaller than in the quiet Sun. This implies that in the region $\tau_5 \ge 1$ the penumbral temperature diverges from that of the quiet Sun. The same result is obtained when focusing only on the thermal structure below the surface of bright penumbral filaments. We interpret these results as evidence of a thick penumbra, whereby the magnetopause is not located near its visible surface. In addition, we find that the temperature gradient in bright penumbral filaments is lower than in granules. This can be explained in terms of the limited expansion of a hot upflow inside a penumbral filament relative to a granular upflow, as magnetic pressure and tension forces from the surrounding penumbral magnetic field hinder an expansion like this.Comment: 5 pages; 2 figures; accepted for publication in Astronomy and Astrophysics Letter

Complex order parameter symmetry and thermal conductivity

Thermal behaviour of superconductors with complex order parameter symmetry is studied within a weak coupling theory. It is shown numerically, that the thermal nature of the different components of complex order parametrs are qualitatively different. Within the complex order parameter scenario, the recent experimental observations by Krishna {\it et al.}, [Science {\bf 277}, 83 (1997)] on magnetothermal conductivity and by J. Ma {\it et al.}, [Science {\bf 267}, 862 (1995)] on temperature dependent gap anisotropy for high temperature superconductors can have natural explanation.Comment: 6 pages, 3 figures and macros attached, Europhysics Letters (1998) in pres

Error threshold in simple landscapes

We consider the quasispecies description of a population evolving in both the "master sequence" landscape (where a single sequence is evolutionarily preferred over all others) and the REM landscape (where the fitness of different sequences is an independent, identically distributed, random variable). We show that, in both cases, the error threshold is analogous to a first order thermodynamical transition, where the overlap between the average genotype and the optimal one drops discontinuously to zero.Comment: 10 pages and 2 figures, Plain LaTe

Mixed-state quasiparticle transport in high-T_c cuprates: localization by magnetic field

Theory of quasiparticle transport in the mixed state of a d-wave superconductor is developed under the assumption of disordered vortex array. A novel universal regime is identified at fields above H*= c*H_{c2}(T/T_c)^2, characterized by a field-independent longitudinal thermal conductivity. It is argued that this behavior is responsible for the high-field plateau in the thermal conductivity experimentally observed in cuprates by Krishana, Ong and co-workers.Comment: 4 pages REVTeX + 1 PostScript figure. Final version to appear in PRL. Several changes in response to referee comments. For related work and info visit http://www.pha.jhu.edu/~fran

Testing replica predictions in experiments

We review the predictions of the replica approach both for the statics and for the off-equilibrium dynamics. We stress the importance of the Cugliandolo-Kurchan off-equilibrium fluctuation-dissipation relation in providing a bridge between the statics and the dynamics. We present numerical evidence for the correctness of these relations. This approach allows an experimental determination of the basic parameters of the replica theory.Comment: To appear in Chiarotti's Festschrift Volume (8 Pages, 3 figures