Sensitivity of Antarctic Urospora penicilliformis (Ulotrichales, Chlorophyta) to ultraviolet radiation is life stage dependent

The sensitivity of different life stages of the eulittoral green alga Urospora penicilliformis (Roth) Aresch. to ultraviolet radiation (UVR) was examinedin the laboratory. Gametophytic filaments and propagules (zoospores and gametes) released from filaments were separately exposed to different fluence of radiation treatments consisting of PAR (P = 400700 nm), PAR + ultraviolet A (UVA) (PA, UVA = 320400 nm), and PAR + UVA + ultraviolet B (UVB) (PAB, UVB = 280320 nm). Photophysiological indices (ETRmax, Ek, and a) derived from rapid light curves were measured in controls, while photosynthetic efficiency and amount of DNA lesions in terms of cyclobutane pyrimidine dimers (CPDs) were measured after exposure to radiation treatments and after recovery in low PAR; pigments of propagules were quantified after exposure treatment only. The photosynthetic conversion efficiency (a) and photosynthetic capacity (rETRmax) were higher in gametophytes compared with the propagules. The propagules were slightly more sensitive to UVB-induced DNA damage; however, both life stages of the eulittoral inhabiting turf alga were not severely affected by the negative impacts of UVR. Exposure to a maximum of 8 h UVR caused mild effects on the photochemical efficiency of PSII and induced minimal DNA lesions in both the gametophytes and propagules. Pigment concentrations were not significantly different between PAR-exposed and PAR + UVRexposed propagules. Our data showed that U. penicilliformis from the Antarctic is ratherinsensitive to the applied UVR. This amphi-equatorial species possesses different protective mechanisms that can cope with high UVR in coldtemperatewaters of both hemispheres and in polar regions under conditions of increasing UVR as a consequence of further reduction of stratosphericozone

UNA MIRADA A TRAVÉS DE LAS MACROALGAS: Una lupa sumergida bajo los mares antárticos, recopilando y compartiendo el conocimiento sobre las macroalgas.

Bajo el mar antártico se esconde una vida única adaptada a condiciones extremas de temperatura y luz. Las macroalgas antárticas desempeñan un papel fundamental en la producción de oxígeno y en la absorción de dióxido de carbono de la atmósfera, contribuyendo a regular el clima global. Estas importantes productoras primarias son auténticas ingenieras del ecosistema marino, crean y modifican los hábitats y proveen refugio y protección a una variedad de organismos marinos. Se presentan aquí las diferentes líneas de investigación desarrolladas en Caleta Potter, un ecosistema marino antártico costero seriamente afectado por los fenómenos asociados al cambio global

Different feeding strategies in scavenging amphipods and their implications for colonisation success in times of retreating glaciers.

Background: Scavenger guilds, composed of a variety of species, co-existing in the same habitat, are responsible for biomass transformation throughout the food web. Niche partitioning among them can manifest in different feeding strategies, e.g. during carcass feeding. In the bentho-pelagic realm of the Southern Ocean, scavenging amphipods of the speciose superfamily Lysianassoidea are amongst the ubiquitous taxa and occupy an essential role in decomposition processes. First, we addressed the question whether scavenging lysianassoid amphipods have different feeding strategies during carcass feeding, and if their potential synergistic feeding activities influence carcass decomposition. To this end, we compared the relatively large-sized species Waldeckia obesa with the small-sized species Cheirimedon femoratus, Hippomedon kergueleni, and Orchomenella rotundifrons during carcass feeding (Notothenia spp.). Our approach combines ex situ feeding experiments, behavioural observations, and scanning electron microscopic analyses of mandibles. Secondly, we aimed to detect ecological drivers for succession patterns of scavenging amphipods in Antarctic coastal ecosystems affected by environmental disturbances. In Potter Cove, the climate-driven rapid retreat of the Fourcade Glacier is causing various environmental changes including the provision of new marine habitats to colonise. While in the newly ice-free areas fish records are rare, macroalgae have already colonised hard substrates. Therefore, we carried out feeding assays of the most abundant lysianassoids in Potter Cove C. femoratus and H. kergueleni, to determine their consumption rates (mg food x mg amphipods-1 x day-1) and preferences of macroalgae and fish. Results We detected two functional groups with different feeding strategies among the investigated scavenging amphipods: the 'outside-insider' (openers) and 'inside-outsider' (squeezers). Synergistic effects during carcass feeding was not statistical evident. C. femoratus showed a flexible diet when fish was not available by consuming macroalgae with a consumption about 0.2 day-1 but preferred fish with feedings rates up to 0.8 day-1. Contrary, H. kergueleni rejected macroalgae entirely and consumed fish with consumption rates up to 0.8 day-1. Conclusion This study reveals functional groups in scavenging shallow water amphipods and provides new information on coastal intraguild niche partitioning. Moreover, we conclude that dietary flexibility of scavenging amphipods is a potential ecological driver for succession and colonisation of newly available ice-free Antarctic coastal habitats

Cryptic Haploid Stages in the Life Cycle of Leathesia marina (Chordariaceae, Phaeophyceae) Under In Vitro Culture

We evaluated the life cycle of Leathesia marina through molecular analyses, culture studies, morphological observations, and ploidy measurements. Macroscopic sporophytes were collected from two localities in Atlantic Patagonia and were cultured under long-day (LD) and short-day (SD) conditions. Molecular identification of the microscopic and macroscopic phases was performed through the cox3 and rbcL genes and the phylogeny was assessed on the basis of single gene and concatenated datasets. Nuclear ploidy of each phase was estimated from the DNA contents of individual nuclei through epifluorescence microscopy and flow cytometry. Molecular results confirmed the identity of the Argentinian specimens as L. marina and revealed their conspecificity with L. marina from New Zealand, Germany, and Japan. The sporophytic macrothalli (2n) released mitospores from plurilocular sporangia, which developed into globular microthalli (2n), morphologically similar to the sporophytes but not in size, constituting a generation of small diploid thalli, with a mean fluorescent nuclei cross-sectional area of 3.21 ± 0.7 μm2. The unilocular sporangia released meiospores that developed two morphologically different types of microthalli: erect branched microthalli (n) with a nuclear area of 1.48 ± 0.07 µm2 that reproduces asexually, and prostrate branched microthalli (n) with a nuclear area of 1.24 ± 0.10 µm2 that reproduces sexually. The prostrate microthalli released gametes in LD conditions, which merged and produced macroscopic thalli with a nuclear cross-sectional area of 3.45 ± 0.09 µm2. Flow cytometry confirmed that the erect and prostrate microthalli were haploid and that the globular microthalli and macrothalli were diploid.Fil: Poza, Ailen Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; ArgentinaFil: Santiañez, Wilfred John E.. Hokkaido University; Japón. University of the Philippines Diliman; FilipinasFil: Croce, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Gauna, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Kogame, Kazuhiro. Hokkaido University; JapónFil: Parodi, Elisa Rosalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentin