The Injector Layout of BERLinPro

BERLinPro is an Energy Recovery Linac Project running since 2011 at the HZB in Berlin. A conceptual design report has been published in 2012 [1]. One of the key components of the project is the 100 mA superconducting RF photocathode gun under development at the HZB since 2010. Starting in 2016 the injector will go into operation, providing 6.6 MeV electrons with an emittance well below 1mm mrad and bunches shorter than 5 ps. In 2017 the 50 MeV linac will be set up and full recirculation is planned for 2018. The injector design has been finalized and is described in detail in this paper. Emphasis is further laid on beam dynamics aspects and performance simulations of two different gun cavitie

A rare missense mutation in <i>GJB3</i> (Cx31G45E) is associated with a unique cellular phenotype resulting in necrotic cell death

Erythrokeratodermia variabilis et progressiva (EKV-P) is caused by mutations in either the GJB3 (Cx31) or GJB4 genes (Cx30.3). We identified a rare GJB3 missense mutation, c.134G>A (p.G45E), in two unrelated patients and investigated its cellular characteristics. Expression of Cx31G45E-GFP caused previously undescribed changes within HeLa cells and HaCaT cells, a model human keratinocyte cell line. Cx31WT-GFP localised to the plasma membrane, but expression of Cx31G45E-GFP caused vacuolar expansion of the endoplasmic reticulum (ER), the mutant protein accumulated within the ER membrane and disassembly of the microtubular network occurred. No ER stress responses were evoked. Cx31WT-myc-myc-6xHis and Cx31G45E-GFP co-immunoprecipitated, indicative of heteromeric interaction, but co-expression with Cx31WT-mCherry, Cx26 or Cx30.3 did not mitigate the phenotype. Cx31 and Cx31G45E both co-immunoprecipitated with Cx43, indicating the ability to form heteromeric connexons. WT-Cx31 and Cx43 assembled into large gap junction plaques at points of cell-to-cell contact; Cx31G45E restricted the ability of Cx43 to reach the plasma membrane in both HaCaT cells and HeLa cells stably expressing Cx43 where the proteins strongly co-localised with the vacolourised ER. Cell viability assays identified an increase in cell death in cells expressing Cx31G45E-GFP, which FACS analysis determined was necrotic. Blocking connexin channel function with 18α-glycyrrhetinic acid did not completely rescue necrosis or prevent propidium iodide uptake, suggesting that expression of Cx31G45E-GFP damages the cellular membrane independent of its channel function. Our data suggest that entrapment of Cx43 and necrotic cell death in the epidermis could underlie the EKV skin phenotype

Influence of boundaries on pattern selection in through-flow

The problem of pattern selection in absolutely unstable open flow systems is investigated by considering the example of Rayleigh-B\'{e}nard convection. The spatiotemporal structure of convection rolls propagating downstream in an externally imposed flow is determined for six different inlet/outlet boundary conditions. Results are obtained by numerical simulations of the Navier-Stokes equations and by comparison with the corresponding Ginzburg-Landau amplitude equation. A unique selection process is observed being a function of the control parameters and the boundary conditions but independent of the history and the system length. The problem can be formulated in terms of a nonlinear eigen/boundary value problem where the frequency of the propagating pattern is the eigenvalue. PACS: 47.54.+r, 47.20.Bp, 47.27.Te, 47.20.KyComment: 8 pages, 5 Postscript figures, Physica D 97, 253-263 (1996

Dissociation constants and thermodynamic properties of amino acids used in CO2 absorption from (293 to 353) K

The second dissociation constants of the amino acids βalanine, taurine, sarcosine, 6-aminohexanoic acid, DL-methionine, glycine, L-phenylalanine, and L-proline and the third dissociation constants of L-glutamic acid and L-aspartic acid have been determined from electromotive force measurements at temperatures from (293 to 353) K. Experimental results are reported and compared to literature values. Values of the standard state thermodynamic properties are derived from the experimental results and compared to the values of commercially available amines used as absorbents for CO 2 capture.

The ABCD of usability testing

We introduce a methodology for tracking and auditing feedback, errors and suggestions for software packages. This short paper describes how we innovate on the evaluation mechanism, introducing an (Antecedent, Barrier, Consequence and Development) ABCD form, embedded within an eParticipation platform to enable end users to easily report on any usability issues. This methodology will be utilised to improve the STEP cloud eParticipation platform (part of the current STEP Horizon2020 project http://step4youth.eu. The platform is currently being piloted in real life contexts, with the participation of public authorities that are integrating the eParticipation platform into their regular decision-making practices. The project is involving young people, through engagement and motivation strategies and giving them a voice in Environmental decision making at the local level. The pilot evaluation aims to demonstrate how open engagement needs to be embedded within public sector processes and the usability methodology reported here will help to identify the key barriers for wide scale deployment of the platform

Results from Beam Commissioning of an SRF Plug Gun Cavity Photoinjector

Superconducting rf photo electron injectors SRF photoinjectors hold the promise to deliver high brightness, high average current electron beams for future light sources or other applications demanding continuous wave operation of an electron injector. This paper discusses results from beam commissioning of a hybrid SRF photoinjector based on a Pb coated plug and a Nb rf gun cavity for beam energies up to 2.5MeV at Helmholtz Zentrum Berlin HZB . Emittance measurements and transverse phase space characterization with solenoid scan and slitmask methods will be presente

Laser Wire Scanner Compton Scattering Techniques for the Measurement of the Transverse Beam Size of Particle Beams at Future Linear Colliders

This archive summarizes a working paper and conference proceedings related to laser wire scanner development for the Future Linear Collider (FLC) in the years 2001 to 2006. In particular the design, setup and data taking for the laser wire experiments at PETRA II and CT2 are described. The material is focused on the activities undertaken by Royal Holloway University of London (RHUL).Comment: 61 page

A cAMP-binding ectoprotein in the yeast Saccharomyces cerevisiae

tides 10, 593-595