Репаративное действие гидрогеля полигексаметиленгуанидин гидрохлорида

Polyguanidines are characterized by pronounced antimicrobial activity and are widely used as the main active substance of disinfectants. Polymers of this class possess the property of gel formation, which, together with biological activity, opens the prospect of obtaining an effective single-component agent or basis for an external preparation for the therapy of skin lesions. Earlier, we developed a method for the production of a hydrogel based on polyhexamethyleneguanidine hydrochloride, which involves the cross-linking of the terminal amino groups of the branched polymer with formaldehyde.The purpose of the study was to observe the effect of hydrogel polyhexamethyleneguanidine hydrochloride on the progression of wound healing.Materials and methods. An experiment on the evaluation of the wound healing action of hydrogel was carried out under conditions simulating a linear cutaneous wound, a muscular wound, and a thermal burn. The comparative drug was the pharmacopoeian drug Levomecol.Results. It has been established that hydrogel has a pronounced wound healing effect, as evidenced by the results of ruminant thoracic anatomy and pathomorphology of the sections, which shows accelerated ripening of granulation tissue and scar formation. By the method of vulnografiya it was shown that the daily decrease in wound area is most pronounced in the hydrogel group, which also indicates a pronounced reparative effect. It was determined that the hydrogel activates the growth of antioxidatic activity and leukocytes in the blood of animals in dynamics on the 10th, 17th and 24th day of the experiment, amounting to 56, 34 and 21%, and of leukocytes – 32, 30, 10% in relation to the intact animals.Conclusion. Thus, on the model of a linear cutaneous wound, a muscular wound, and a thermal burn, the healing effect of the hydrogel PGMGh/f is established, as evidenced by the results of the early exercise, vulnografiya and pathomorphological studies. It is noted that the hydrogel PGMG h/f has an effect on the content of antioxidants and leukocytes in the blood, contributing to the normalization of their quantity.Полигуанидины характеризуются выраженной антимикробной активностью и находят широкое применение в качестве основного действующего вещества в составе дезинфицирующих средств. Полимеры этого класса обладают свойством гелеобразования, что в совокупности с биологической активностью открывает перспективу получения эффективного однокомпонентного средства или основы препарата наружного применения для терапии повреждений кожных покровов. Ранее нами был разработан способ получения гидрогеля на основе полигексаметиленгуанидин гидрохлорида, заключающийся в сшивании концевых аминогрупп разветвленного полимера формальдегидом.Целью работы явилось изучение влияния гидрогеля полигексаметиленгуанидин гидрохлорида на течение раневого процесса.Материалы и методы. Эксперимент по оценке ранозаживляющего действия гидрогеля проводился в условиях моделирования линейной кожно-мышечной раны и термического ожога. В качестве сравнения использовался фармакопейный препарат «Левомеколь».Результаты. Установлено, что гидрогель обладал выраженным ранозаживляющим действием на модели линейной раны, о чем свидетельствовали результаты ранотензометрии рубца и патоморфологии срезов, показавшие ускоренное созревание грануляционной ткани и формирование рубца. Методом вульнографии на модели термического ожога показано, что суточное уменьшение площади раны в наибольшей степени эксплицировано у опытной группы 1 (гидрогель), что также свидетельствовало о его выраженном репаративном действии. Определено, что при ожоге наблюдались резкое уменьшение суммарной антиоксидантной активности сыворотки крови и нарастание количества лейкоцитов. В динамике на 10-, 17- и 24-е сут эксперимента уменьшение первого показателя составило 56, 34 и 21%, а увеличение второго показателя – соответственно на 32, 30 и 10% по отношению к интактным животным. Применение гидрогеля более эффективно, чем препарата сравнения, способствовало нормализации данных показателей.Заключение. Таким образом, на моделях линейной кожно-мышечной раны и термического ожога установлено выраженное ранозаживляющее действие гидрогеля, о чем свидетельствовали результаты ранотензометрии, вульнографии патоморфологических исследований. Более высокий ранозаживляющий эффект гидрогеля, по-видимому, связан с увеличением суммарной антиоксидантной активности и более выраженным восстановлением количества лейкоцитов в крови

Reparative action of hydrogel polygexamethylenuanidine hydrochloride

Polyguanidines are characterized by pronounced antimicrobial activity and are widely used as the main active substance of disinfectants. Polymers of this class possess the property of gel formation, which, together with biological activity, opens the prospect of obtaining an effective single-component agent or basis for an external preparation for the therapy of skin lesions. Earlier, we developed a method for the production of a hydrogel based on polyhexamethyleneguanidine hydrochloride, which involves the cross-linking of the terminal amino groups of the branched polymer with formaldehyde.The purpose of the study was to observe the effect of hydrogel polyhexamethyleneguanidine hydrochloride on the progression of wound healing.Materials and methods. An experiment on the evaluation of the wound healing action of hydrogel was carried out under conditions simulating a linear cutaneous wound, a muscular wound, and a thermal burn. The comparative drug was the pharmacopoeian drug Levomecol.Results. It has been established that hydrogel has a pronounced wound healing effect, as evidenced by the results of ruminant thoracic anatomy and pathomorphology of the sections, which shows accelerated ripening of granulation tissue and scar formation. By the method of vulnografiya it was shown that the daily decrease in wound area is most pronounced in the hydrogel group, which also indicates a pronounced reparative effect. It was determined that the hydrogel activates the growth of antioxidatic activity and leukocytes in the blood of animals in dynamics on the 10th, 17th and 24th day of the experiment, amounting to 56, 34 and 21%, and of leukocytes – 32, 30, 10% in relation to the intact animals.Conclusion. Thus, on the model of a linear cutaneous wound, a muscular wound, and a thermal burn, the healing effect of the hydrogel PGMGh/f is established, as evidenced by the results of the early exercise, vulnografiya and pathomorphological studies. It is noted that the hydrogel PGMG h/f has an effect on the content of antioxidants and leukocytes in the blood, contributing to the normalization of their quantity

Microstructure and Properties of an Electrocontact Cu–(ZnO/TiO2) Material

Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.Abstract—A copper-based electrical-contact composite material hardened by disperse zinc oxide and zinc titanate is studied by electron microscopy and energy dispersive X-ray macroanalysis. The distribution of oxide phases in the samples containing 2.5 wt % oxide nanopowder mixture in an initial charge is found to be uniform. An increase in the amount of oxides leads to an increase in their sized in sintering. A relation between the sample wear and the sample composition is obtained during laboratory tests. It is shown that the introduction of more than 2.5 wt % oxide mixture results in intense wear of the working surface of the sample and an increase in the running-in period of contacts

Microstructure and Properties of an Electrocontact Cu–(ZnO/TiO2) Material

Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала.Abstract—A copper-based electrical-contact composite material hardened by disperse zinc oxide and zinc titanate is studied by electron microscopy and energy dispersive X-ray macroanalysis. The distribution of oxide phases in the samples containing 2.5 wt % oxide nanopowder mixture in an initial charge is found to be uniform. An increase in the amount of oxides leads to an increase in their sized in sintering. A relation between the sample wear and the sample composition is obtained during laboratory tests. It is shown that the introduction of more than 2.5 wt % oxide mixture results in intense wear of the working surface of the sample and an increase in the running-in period of contacts

Possibilities of using tear fluid for electron microscopic diagnostics of eye diseases

Purpose. To conduct a comparative study of cellular and subcellular components of tear fluid of healthy persons (HP), those suffering from primary open-angle glaucoma (POAG), and patients with diabetic retinopathy (DR). Material and methods. Electron microscopy of ultrathin sections was used to test tear samples of 16 patients with initial POAG, 9 patients with developed POAG, and 6 with an advanced stage of POAG. Besides, 12 patients with proliferative DR (PD) and 6 patients with nonproliferative DR (NPD) were tested. Tear samples of 29 subjects with no ophthalmic pathology served as control. Results. All the examined samples contained cells, cellular fragments and debris, mucous granules and lipid structures. The occurrence of all these structures was found to be different in the tears of diabetic and glaucomatous patients as compared to that of HP. The share of epithelial cells in the tear of patients with the initial stage of POAG was twice as high as in the control group; then it drops gradually so that in the advanced stage, epithelial cells are not detected. The tear of NPD patients shows an increase of the content of mucin and lipids against the control, while in PD it is lower than in HP. Leukocytes were observed in the tear of a quarter of HP, while in diabetic patients leukocytes show a significant increase, which reflect a higher permeability of blood vessels as the disease develops. There were no leukocytes in tear samples of all patients with POAG, which may be associated with damaged functions of the blood vessel wall. We found “hairy” vesicles in the tear which had not been noted previously; the amount of these vesicles was growing at early stages of POAG and DR. Conclusion. Electron microscopy of tear deposits evaluates the state of the cornea and the conjunctiva, as well as goblet cells and the meibomian glands. It reveals signs of permeability change in tissue vessels adjacent to the anterior area of the eye. For the first timechanges in tear components in glaucomatous and diabetic patients, were found: theses changes may in future be used to achieve early diagnosis of these diseases // Russian Ophthalmological Journal, 2017; 1: 56-62. doi: 10.21516/2072-0076-2017-10-1-56-62