Effect of integrated nitrogen management through organic and inorganic sources on the yield of rice (Oryza sativa L.) and status of soil fertility at harvest

The present investigation was conducted with the objective of utilizing locally available organic resources for substituting chemical fertilizer nitrogen partly and augmenting soil health.  The experiment was carried out for the crop kharif rice (Oryza sativa L.) in coastal Karnataka with eleven treatments laid out in randomized block design with three replications. Vermicompost (VC), Poultry manure (PM) and Fish manure (FM) were used as organic nitrogen sources integrated with mineral nitrogen source of fertilizer urea at substitution ratios of  25, 50 and 100 percent. These treatments were compared with control and recommended dose of nitrogen. The results revealed that the substitution of 50 per cent of nitrogen through VC  recorded significantly (P<0.05) higher plant height  (92.27 cm), higher number of productive tillers (16.85 hill-1), higher grain yield (5434 kg ha-1) and straw yield ( 6817 kg ha-1) compared to control. The results of the soil sample analysis for soil fertility parameters indicated that the major nutrients were significantly (P<0.05) influenced by integrated nitrogen management.  The available nitrogen (382.36 kg ha-1) and available potassium (143.7 kg ha-1) were significantly (P<0.05) higher in the treatment T4 where a recommended dose of nitrogen was substituted by 50 percent VC compared to control (294.05 kg ha-1 and 92.31 kg ha-1 respectively). However, the available soil phosphorus was significantly higher with treatment 50 per cent  RDN substituted by PM  (78.83 kg ha-1) as compared to control (43.93 kg  ha-1).  Thus, integrated resource management improved crop yield and post-harvest soil fertility

Novel role for alpha-2-macroglobulin (A2M) as a disease modifying protein in senile osteoporosis

Introduction: In the rapidly aging U.S. population, age-induced bone loss (senile osteoporosis) represents a major public health concern that is associated with a significant increased risk for low trauma fragility fractures, which are debilitating to patients, cause significant morbidity and mortality, and are costly to treat and manage. While various treatments exist to slow bone loss in osteoporosis patients, these suffer from poor tolerability and label restrictions that limit their overall effectiveness. Over the past decade, skeletal stem/progenitor cells (SSPCs), which are the main precursor of osteoblasts and adipocytes in adult bone marrow (BM), have emerged as important players in osteoporosis.Methods: Age-induced skeletal pathology was quantified in elderly (24-month-old) vs. mature (3-month-old) mice by micro-CT and changes in SSPC abundance in the BM of these mice was quantified by fluorescence-activated cell sorting (FACS). SSPCs from elderly vs. mature mice were also analyzed by RNA-Seq to identify differentially expressed genes (DEGs), and gain and loss-of-function studies were performed in human BM-derived mesenchymal stromal cells (BM-MSCs) to assess A2M function.Results: Elderly mice were shown to exhibit significant age-induced skeletal pathology, which correlated with a significant increase in SSPC abundance in BM. RNA-seq analysis identified alpha-2-macroglobulin (A2M), a pan-protease inhibitor that also binds inflammatory cytokines, as one of the most downregulated transcripts in SSPCs isolated from the BM of elderly vs. mature mice, and silencing of A2M expression in human BM-MSCs induced their proliferation and skewed their lineage bifurcation toward adipogenesis at the expense of osteogenesis thereby recapitulating critical aspects of age-induced stem cell dysfunction.Conclusion: These findings identify A2M as a novel disease modifying protein in osteoporosis, downregulation of which in bone marrow promotes SSPC dysfunction and imbalances in skeletal homeostasis

Pleomorphic adenoma with extensive squamous metaplasia and keratin cyst formations in minor salivary gland: a case report

Pleomorphic adenoma (PA), the most common salivary gland tumor, accounts for 54 to 65% of all salivary gland neoplasias and 80% of the benign salivary gland tumors. It most frequently affects the parotid gland, followed by the submandibular and the minor salivary glands. Microscopically, mucous, sebaceous, oncocytic and squamous metaplasia, sometimes with the formation of keratin pearls, may be present, but the latter rarely results in the formation of extensive keratin-flled cysts lined by squamous epithelium. Extensive squamous metaplasia can be mistaken for malignancy, including mucoepidermoid carcinoma and squamous cell carcinoma. Here, we present an unusual case of PA with extensive squamous metaplasia and keratin cyst formations in a minor salivary gland, and discuss its microscopic features, including the immunohistochemical characteristics, and differential diagnosis of this uncommon presentation

Effect of Butea monosperma Lam. leaves and bark extracts on blood glucose in streptozotocin-induced severely diabetic rats

Background: Diabetes mellitus is a chronic metabolic disorder that has significant impact on the health, quality of life and life expectancy, as well as on the health care system. Butea monosperma Lam. Kuntze (Fabaceae), commonly known as palash, is widely used in the treatment of various diseases and disorders including diabetes. Materials and Methods: The present study was planned to evaluate the antidiabetic effect of aqueous extracts of B. monosperma leaves and bark in streptozotocin-induced severely diabetic rats. The animals were divided into four groups, with each consisting of six rats, viz. control, diabetic control, leaf extract-treated and bark extract-treated groups. Treatment was continued for 6 weeks. The biochemical estimations included blood glucose and serum insulin levels. Histopathology of pancreas was also performed. Results: The results indicated that both leaf and bark extracts of B. monosperma produced insignificant antihyperglycemic activity. The leaf and bark extracts reduced blood glucose to an extent of 28% and 11%, respectively. It was also evidenced that both leaf and bark extracts did not increase insulin synthesis or secretion and did not improve pancreatic architecture as reflected by the histopathologic studies. Conclusions: The findings of the study emphasize that B. monosperma does not possess significant antidiabetic activity in severe experimental diabetes at the dosage tested