Presence of multiple bacterial markers in clinical samples might be useful for presumptive diagnosis of infection in cirrhotic patients with culture-negative reports

Bacterial infections in cirrhotic patients with ascites are associated with a severe prognosis and an increased risk of death. The microbiological standard tests for the diagnosis of suspected infection, based on culture test of blood and ascitic fluid, are, in many cases (30-40 %), negative, even when patients show symptoms of infection. A multiple culture-independent protocol was applied and evaluated as a diagnostic and prognostic tool for the detection of bacterial infection in cirrhotic patients. Sixty-four culture-negative samples obtained from 34 cirrhotic patients, with PMN < 250 cells/μl of ascitic fluid, were screened for the presence of bacterial DNA, endotoxin, peptidoglycan/β-glucan and microscopically visible bacterial cells. Correlations between the presence of multiple markers and various clinical and laboratory parameters were evaluated. Bacterial DNA was detected in 23 samples collected from 16 patients; a large part of these samples also showed the presence of other bacterial markers, which was associated with a worsening of liver functionality, a higher incidence of infections during the follow-up and a higher mortality rate in our cohort of cirrhotic patients. We believe that the detection of additional bacterial markers in bacterial DNA-positive clinical samples makes the bacterial presence and its clinical significance more realistic and might be useful as early markers of an ongoing bacterial infection and in establishing a clinical prognosis

Proving strong magnetic fields near to the central black hole in the quasar PG0043+039 via cyclotron lines

The optical luminous quasar PG0043+039 has not been detected before in deep X-ray observations indicating the most extreme optical-to-X-ray slope index ${\alpha}_{ox}$ of all quasars. This study aims to detect PG0043+039 in a deep X-ray exposure. Furthermore, we wanted to check out whether this object shows specific spectral properties in other frequency bands. We took deep X-ray (XMM-Newton), far-ultraviolet (HST), and optical (HET, SALT telescopes) spectra of PG0043+039 simultaneously in July 2013. We just detected PG0043+039 in our deep X-ray exposure. The steep ${\alpha}_{ox} = -2.37 {\pm} 0.05$ gradient is consistent with an unusual steep gradient $F_{\nu} {\sim} {\nu}^{\alpha}$ with ${\alpha} = -2.67 {\pm} 0.02$ seen in the UV/far-UV continuum. The optical/UV continuum flux has a clear maximum near 2500 {\AA}. The UV spectrum is very peculiar because it shows broad humps in addition to known emission lines. A modeling of these observed humps with cyclotron lines can explain their wavelength positions, their relative distances, and their relative intensities. We derive plasma temperatures of T ${\sim}$ 3keV and magnetic field strengths of B ${\sim}$ 2 ${\times} 10^8$ G for the line-emitting regions close to the black hole.Comment: 4 pages, 3 figures, Astronomy & Astrophysics in pres

The XMM-Newton view of PG quasars: II. Properties of the Fe K-alpha line

The properties of the fluorescence Fe K-alpha emission lines of a sample of 38 quasars (QSOs) observed with XMM-Newton are studied. These objects are included in the optically selected sample from the Palomar-Green (PG) Bright Quasar Survey with an X-ray luminosity 1.3E43<L(2-10 keV)<5.1E45 ergs/s and z<1.72. For each object in the sample, we investigated the presence of both narrow and broad iron lines in detail. A total of 20 out of the 38 QSOs show evidence of an Fe K-alpha emission line with a narrow profile. The majority of the lines are consistent with an origin in low ionization material, which is likely to be located in the outer parts of the accretion disk, the molecular torus, and/or the Broad Line Region. The average properties of the narrow Fe K-alpha emission line observed in the sample are similar to those of Seyfert type galaxies as inferred from recent XMM-Newton and Chandra studies. A broad line has been significantly detected in only three objects. Furthermore, we studied the relationship between the equivalent width (EW) of the iron line and the hard band X-ray luminosity for radio quiet quasars. The analysis indicates that no clear correlation between the strength of the line and the hard X-ray luminosity is present, and our results do not show compelling evidence for an anticorrelation between these two quantities, i.e. the so-called X-ray Baldwin effect.Comment: 10 pages, 3 figures, accepted by A&

A long hard look at the minimum state of PG 2112+059 with XMM-Newton

XMM-Newton successfully detected the minimum state of PG 2112+059 during a short snapshot observation and performed a long follow-up observation. The high signal-to-noise spectra are modelled assuming different emission scenarios and compared with archival spectra taken by XMM-Newton and Chandra. The PG 2112+059 X-ray spectra acquired in May 2007 allowed the detection of a weak iron fluorescent line, which is interpreted as being caused by reflection from neutral material at some distance from the primary X-ray emitting source. The X-ray spectra of PG 2112+059 taken at five different epochs during different flux states can be interpreted within two different scenarios. The first consists of two layers of ionised material with column densities of N_H ~5 x 10^22 cm^-2 and N_H ~3.5 x 10^23 cm^-2, respectively. The first layer is moderately ionised and its ionisation levels follow the flux changes, while the other layer is highly ionised and does not show any correlation with the flux of the source. The spectra can also be interpreted assuming reflection by an ionised accretion disk seen behind a warm absorber. The warm absorber ionisation is consistent with being correlated with the flux of the source, which provides an additional degree of self-consistency with the overall reflection-based model. We explain the spectral variability with light bending according to the models of Miniutti and Fabian and constrain the black hole spin to be a/M > 0.86. Both scenarios also assume that a distant cold reflector is responsible for the Fe K \alpha emission line. Light bending provides an attractive explanation of the different states of PG 2112+059 and may also describe the physical cause of the observed properties of other X-ray weak quasars.Comment: 15 pages, 12 figures, A&A latex, accepted for publication in Astronomy & Astrophysic

Isolation of a Vibrio parahaemolyticus pandemic strain from a marine water sample obtained in the northern Adriatic

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