Comparative Study of Some Physical-Chemical and Immunochemical Properties of Plague Microbe Lipopolysaccharide Preparations Obtained with the Help of Different Techniques

, and degraded polysaccharide (PS) are easily soluble in water and in 0,9 % NaCl solution. They are homogenous and characterized by an adequate degree of purity. Aside from that, it is demonstrated that potentially PS is the most productive molecule fragment of LPS for the construction of plague immunodiagnostic preparation, since despite its decreased cytotoxocity PS retains identity of chemical composition and immunechemical specificity of endotoxin

Egg retention and dispersal activity in the parasitoid wasp, Trichogramma principium

Effects of egg retention on movement and dispersal activity of Trichogramma principium (Hymenoptera, Trichogrammatidae) females were investigated under laboratory conditions. Individual females were observed during one minute in the absence of hosts. Movement activity and dispersal rate were estimated by the length of the track and by the distance from the start point, respectively. Before the test, all wasps during 2 – 4 days were presented with a possibility to parasitize a factitious laboratory host, Sitotroga cerealella Oliv. (Lepidoptera, Gelechiidae). Wasps that had parasitized before the test show significant reduction of spontaneous walking activity and dispersal rate when compared with females that refused to parasitize the non-preferred host (i.e. manifested egg retention). This effect cannot be considered as a direct arrestment reaction to the host because during the test period, no hosts were provided. Thus, egg retention results not only in temporal spread, but also in more intensive spatial dispersal of a group of simultaneously emerged females

Cellular Immune Status Indicators of Anti-Plague Vaccinated Persons, Living on the Caspian Sandy Natural Plague Focus Territory

Aim. To compare by flow cytometry the cellular immune status indicators in persons, living on the territory of the Caspian sandy natural plague focus for epidemic indications. Materials and methods. Whole blood leukocytes of 120 people were examined by flow cytometry with using the two four-color labeled monoclonal antibody reagents (Cyto-Stat CD45-FITC, CD4-PE, CD8-ECD, CD3-PC5 и Cyto-Stat CD45-FITC, CD56-PE, CD19-ECD, CD3-PC5) and by means of CD95-FITC reagent. The results were taken into account before and in a month and 6 months after vaccination. We were determined in the blood the relative content of lymphocytes, monocytes, granulocytes, leukocytes carrying an early apoptosis marker (CD95), T and B- lymphocytes, NK cells as well T – lymphocyte helpers (Th) and cytotoxic T-lymphocytes (Tcyt) for immunoregulatory index (IRI) calculation. Results. Before anti-plague vaccination in 14 people (11,7%) were found abnormally low immunoregulatory index values characterized the immunodeficiency state (IRI <1) and noted less intensive and less prolonged immune system cell reaction in response to anti-plague vaccination. In the other surveyed persons IRI were registered before vaccination in the range 1,4-1,5 with a smooth increase in it a month after vaccination and with preservation of the established IRI interval in a range 1,6-1,8 later 6 months after an inoculation. Conclusion. To estimate the effectiveness of anti plague vaccination within the framework of the ongoing measures quality monitoring to ensure sanitary and epidemiological welfare of the population, living on territory of the natural plague foci, the timely immune status monitoring is important in persons at risk for plague infection (hunters, shepherds, agricultural workers, medical workers)