Reliability of BOD POD Measurements Remain High Following a Short Duration Low-Carbohydrate Diet

The purpose of the present study was to determine whether expected changes in body weight via a three day low-carbohydrate (CHO) diet will disrupt the reliability of air displacement plethysmography measurements via BOD POD. Twenty-four subjects recorded their typical diets for three days prior to BOD POD and seven-site skinfold analyses. Subjects were matched for lean body mass and divided into low-CHO (LC) and control (CON) groups. The LC group was given instruction intended to prevent over 50 grams/day of carbohydrate consumption for three consecutive days, while the CON group replicated their previously recorded diet. Body composition measurements were repeated post-dietary intervention. Test -retest reliability measures were significant (p \u3c 0.01) and high for body fat percentage in both the LC and CON groups (r = 0.993, 0.965, respectively). Likewise, skinfold analysis for body fat percentage reliability was high in both groups (r = 0.996, 0.997, respectively). There were significant differences between first and second BOD POD measurements for body mass (72.9 ± 13.3 vs. 72.1 ± 13.0 kg) and body volume (69.0 ± 12.7 to 68.1 ± 12.2 L) in the LC group (p \u3c 0.05). However, there were no differences (p \u3e 0.05) in BOD POD-determined body fat percentage, lean body mass, or fat mass between the first and second trial in either groups. Body composition measures via BOD POD and seven-site skinfolds remain reliable after three days of a LC diet despite significant decreases in body mass

Identification And Characterization Of A Second Encephalitogenic Determinant Of Myelin Proteolipid Protein (Residues 178-191) For SJL Mice

We previously described a synthetic peptide of myelin proteolipid protein (PLP), peptide 139-151, which induces experimental allergic encephalomyelitis in SJL/J (H-2s) mice. We have now identified an additional determinant, PLP residues 178-191, that is also a potent encephalitogen in this strain. When PLP peptide 178-191 was compared with peptide 139-151 on an equimolar basis, the day of onset of disease induced by PLP 178-191 was earlier, but the incidence, severity, and histologic features were indistinguishable. Lymph node cells from animals immunized with the whole PLP molecule responded to both PLP 178-191 and 139-151, suggesting immunologic codominance of the two epitopes. PLP 178-191 elicited stronger proliferative responses and this may relate to the earlier onset of disease induced with this peptide. Two CD4+, peptide-specific, I-A(s)-restricted T cell lines, selected by stimulation of lymph node cells with either PLP 178-191 or 139-151, were each encephalitogenic in naive syngeneic mice. The presence of multiple encephalitogenic codominant PLP epitopes within a single mouse strain emphasizes the complexity of the immune response to PLP and its potential as a target Ag in autoimmune demyelinating diseases

First Evidence of Persistent Nighttime Temperature Structures in the Neutral Thermosphere of Mars

Using two Mars years of data collected by the Neutral Gas and Ion Mass Spectrometer on the Mars Atmosphere and Volatiles EvolutioN spacecraft, we reconstruct the local solar time structure of the Martian equatorial thermosphere for the dawn and dusk sectors. The results indicate the presence of several persistent features near the dusk and dawn terminators appearing in the neutral temperature and in the O, Ar, N2, and CO2 densities. The dusk temperature features include a minimum at the terminator surrounded by two local maxima with amplitudes between 20 and 40 K. A nighttime temperature enhancement occurs at a local solar time of 4–5 hr and has an amplitude between 50 and 100 K relative to the surrounding temperatures. The observed enhancements are interpreted to be a result of either nighttime dynamical heating caused by converging and downwelling winds or of a terminator wave originating in the lower atmosphere.Plain Language SummaryNASA’s Mars Atmosphere and Volatiles EvolutioN (MAVEN) spacecraft has been collecting information about the Mars’ upper atmosphere since November 2014. In our study we examined the temperature in the upper atmosphere measured by a gas analyzer on MAVEN that periodically sniffs the air around it. By combining several years of data, we reconstructed a map of atmospheric temperatures dividing it into equatorial daytime, morning, evening, and nighttime regions. The results indicate that local patches of warm atmosphere extend about 300–600 km along the Martian equator (3–6 hr driving distance at highway speeds) on the nightside near morning and evening. One possible reason for these nighttime warm spots is the air currents in the Martian upper atmosphere. As the sun heats the dayside atmosphere, it expands and causes wind currents to blow away from the dayside region. These wind currents can extend away from the equator then return on the nightside colliding in the morning and evening regions. The resulting meeting of wind currents can heat the upper atmosphere. Warmer air escapes from the upper atmosphere faster. Because of this, these nighttime patches of warm air could have contributed to a faster escape of Martian atmospheric gases than previously believed.Key PointsPersistent neutral density and temperature enhancements are observed within 30 min of the dusk terminatorA persistent neutral density enhancement is also observed prior to the dawn terminator at around 4–5 local timeThe neutral density features are observed in all species and appear to be associated with enhancements in neutral temperaturePeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146482/1/grl57932.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/146482/2/grl57932_am.pd

Slip statistics of dislocation avalanches under different loading modes

Slowly compressed microcrystals deform via intermittent slip events, observed as displacement jumps or stress drops. Experiments often use one of two loading modes: an increasing applied stress (stress driven, soft), or a constant strain rate (strain driven, hard). In this work we experimentally test the influence of the deformation loading conditions on the scaling behavior of slip events. It is found that these common deformation modes strongly affect time series properties, but not the scaling behavior of the slip statistics when analyzed with a mean-field model. With increasing plastic strain, the slip events are found to be smaller and more frequent when strain driven, and the slip-size distributions obtained for both drives collapse onto the same scaling function with the same exponents. The experimental results agree with the predictions of the used mean-field model, linking the slip behavior under different loading modes

Policy instruments in the Common Agricultural Policy

Policy changes in the Common Agricultural Policy (CAP) can be explained in terms of the exhaustion and long-term contradictions of policy instruments. Changes in policy instruments have reoriented the policy without any change in formal Treaty goals. The social and economic efficacy of instruments in terms of evidence-based policy analysis was a key factor in whether they were delegitimized. The original policy instruments were generally dysfunctional, but reframing the policy in terms of a multifunctionality paradigm permitted the development of more efficacious instruments. A dynamic interaction takes place between the instruments and policy informed by the predominant discourses

Increased Immunoreactivity to Two Overlapping Peptides of Myelin Proteolipid Protein in Multiple Sclerosis

We tested the proliferative responses of peripheral blood mononuclear cells from 61 patients with multiple sclerosis, 56 healthy control subjects and 52 patients with other neurological diseases to seven synthetic peptides of myelin proteolipid protein (PLP) and 19 synthetic peptides of myelin basic protein (MBP). Increased proliferative responses to two overlapping PLP peptides, PLP184-199 and PLP190-209, were found significantly more frequently in blood from patients with relapsing-remitting or secondary progressive multiple sclerosis (52.3%), but not from those with primary progressive multiple sclerosis (18.2%), than in that from healthy control subjects (8.9%) and patients with other neurological diseases (20.8%). Reactivity to these PLP peptides was most frequently seen in blood from patients with multiple sclerosis of 6-15 years duration and with moderate to severe disability (Kurtzke's Expanded Disability Status Scale > 4.0); the blood from 15 of 19 patients in this group reacted to one or both of the peptides. Both peptides could be recognized by short-term T-cell lines specific for whole PLP, and lines specific for one or other of the two overlapping peptides were able to recognize whole PLP, indicating that these peptides can be processed naturally from the intact molecule. This region of PLP is encephalitogenic in a number of strains of mice. Samples from multiple sclerosis patients did not react more frequently to any of the MBP peptides than those from healthy control subjects. The proportions of patients with other neurological diseases whose blood responded to the MBP peptides that most frequently elicited responses in blood from multiple sclerosis patients were significantly lower than the proportions of multiple sclerosis patients and healthy control subjects whose blood responded to these peptides

Advanced ultra-light multifunctional metallic-glass wave springs

We show that, using thermo -elastic processing, metallic -glass foils can be shaped, without being embrittled, into linear and annular wave springs. These springs exhibit an undulatory behaviour, unique to metallic -glass foils, in which under compression the number of arcs in the spring increases, increasing the load -bearing capacity and the spring constant. We evaluate the performance limits of the metallic -glass wave springs, and consider how the undulatory behaviour can be exploited. The metallic -glass springs can operate over the same load -ranges as commercially available crystalline wave springs, but have material volumes (and therefore weights) that are one to two orders of magnitude less. Their energy storage per unit material volume is as high as 2600 kJ m – 3 . We suggest that the undulatory behaviour is important in rendering the springs fail -safe in case of overload. We discuss the range of applicability of thermo -elastic processing, the likely working limit of metallic -glass wave springs, and the potential for application of metallic -glass springs in MEMS devices

Integrating sexual and reproductive health into health system strengthening in humanitarian settings: a planning workshop toolkit to transition from minimum to comprehensive services in the Democratic Republic of Congo, Bangladesh, and Yemen.

BackgroundPlanning to transition from the Minimum Initial Service Package for Sexual and Reproductive Health (SRH) toward comprehensive SRH services has been a challenge in humanitarian settings. To bridge this gap, a workshop toolkit for SRH coordinators was designed to support effective planning. This article aims to describe the toolkit design, piloting, and final product.MethodsAnchored in the Health System Building Blocks Framework of the World Health Organization, the design entailed two complementary and participatory strategies. First, a collaborative design phase with iterative feedback loops involved global partners with extensive operational experience in the initial toolkit conception. The second phase engaged stakeholders from three major humanitarian crises to participate in pilot workshops to contextualize, evaluate, validate, and improve the toolkit using qualitative interviews and end-of-workshop evaluations. The aim of this two-phase design process was to finalize a planning toolkit that can be utilized in and adapted to diverse humanitarian contexts, and efficiently and effectively meet its objectives. Pilots occurred in the Democratic Republic of Congo for the Kasai region crisis, Bangladesh for the Rohingya humanitarian response in Cox's Bazar, and Yemen for selected Governorates.ResultsResults suggest that the toolkit enabled facilitators to foster a systematic, participatory, interactive, and inclusive planning process among participants over a two-day workshop. The approach was reportedly effective and time-efficient in producing a joint work plan. The main planning priorities cutting across settings included improving comprehensive SRH services in general, healthcare workforce strengthening, such as midwifery capacity development, increasing community mobilization and engagement, focusing on adolescent SRH, and enhancing maternal and newborn health services in terms of quality, coverage, and referral pathways. Recommendations for improvement included a dedicated and adequately anticipated pre-workshop preparation to gather relevant data, encouraging participants to undertake preliminary study to equalize knowledge to partake fully in the workshop, and enlisting participants from marginalized and underserved populations.ConclusionCollaborative design and piloting efforts resulted in a workshop toolkit that could support a systematic and efficient identification of priority activities and services related to comprehensive SRH. Such priorities could help meet the SRH needs of communities emerging from acute humanitarian situations while strengthening the overall health system

Scotland, Catalonia and the “right” to self-determination: a comment suggested by Kathryn Crameri’s “Do Catalans Have the Right to Decide?

No abstract available

The functional analysis of nonsense suppressors derived from in vitro engineered \u3ci\u3eSaccharomyces cerevisiae\u3c/i\u3e tRNA\u3csup\u3eTrp\u3c/sup\u3e genes

Nonsense suppressors derived from Saccharomyces cerevisiae tRNATrp genes have not been identified by classical genetic screens, although one can construct efficient amber (am) suppressors from them by making the appropriate anticodon mutation in vitro. Herein, a series of in vitro constructed putative suppressor genes was produced to test if pre-tRNATrp processing difficulties could help to explain the lack of classical tRNATrp-based suppressors. It is clear that inefficient processing of in-trons from precursor tRNATrp, or inaccurate overall processing, may explain why some of these constructs fail to promote nonsense suppression in vivo. However, deficient processing must be only one of the reasons why classical tRNATrp-based suppressors have not been characterized, as suppres-sion may still be extremely weak or absent in instances where the in vitro construct can lead to an accumulation of mature tRNATrp. Furthermore, suppression is also very weak in strains transformed with an intronless derivative of a putative tRNATrp ochre (oc) suppressor gene, wherein intron removal cannot pose a problem