Sadržaj minerala u kostima mijasteničara

Bone mineral content (BMC) was measured in 23 patients with myasthenia gravis using the 241Am gamma ray attenuation method. Measurements were performed in the proximal (BMC6) and distal (BMC1) parts of the forearm, i.e. at 6 and 1 cm distance from the styloid process of the ulna in supine position. At 6 cm from the styloid process the bone is mostly compact, whereas at 1 cm distance it is mostly trabecular. The results were compared with the BMC values in age and sex matched controls. No signs of major bone loss were noticed in any of the myasthenic patients examined in spite of prolonged administration of fluorocortolone.Sadržaj minerala kosti (BMC) podlaktice mjeren je metodom atenuacije (gašenja) gama zraka 241Am u 23 pacijenta (18 žena i 5 muškaraca). Sadržaj minerala u kosti određivanje u proksimalnom (BMC6) i distalnom (BMC1) dijelu podlaktice. Rezultati mjerenja uspoređivani su s BMC vrijednostima odgovarajućih dobnih skupina normalne populacije. Nisu zapaženi znakovi značajnijeg gubitka kosti kod osoba oboljelih od mijastenije gravis unatoč produženoj terapiji fluorokortolonom

A Gel-Chromatographic and Light Scattering Study of the Salmonella typhi Endotoxin

The endotoxin of Salmonella typhi, strain 0-901, was isolated by extraction with hypertonic (1 M) sodium chloride solutions and studied by gel-chromatography and light scattering methods. The gel-chromatographic separation was performed on Sepharose 2B, Sepharose 4B and Sephadex G-200 gels, and the fractionated material was monitored by ultraviolet and phenol-sulfuric acid colorimetry as well as by a photometric latex agglutination test. The extracted material consisted of two components: one was the high molecular weight endotoxin and the other a protein-polysaccharide complex of a molecular weight lower than 66,000 dalton. The light scattering experiments of endotoxin extracts showed the average molecular weights from 1.9 to 4.9 million dalton. The separation of the low molecular weight proteinic component was attempted by thermal denaturation, but this had to be abandoned owing to the denaturation and degradation of endotoxin. A high molecular weight endotoxin component was isolated by elution on a Sephadex G-200 column and had the molecular weight of 5.6 million dalton, which was in good accord with the value previously determined for a Boivin extraction sample. The high molecular weight endotoxin sample proved to be a highly polydispersed material. From the estimates of various averages of gyration radii it has been concluded that the particles of this sample have a more compact structurE than those of the Boivin extraction sample, possibly due to the tertiary structuring of polypeptide chains in the protein-lipopolysaccharide complex of the endotoxin particle

The Isolation and Kinetics of Lactoperoxidase

The catalatic and peroxidatic activities of lactoperoxidase and horse-ra dish peroxidase are described. For measuring the peroxidatic activity. pyrogallol was used as a hydrogen donor. A manometric m ethod was used for the determination of the Km value. The Km value for the catalatic reaction was calculated on the basis of 0 2 evolved, a nd the peroxidatic reaction was calculated on the basis of C02 evolved. In the catalatic reaction the efficiency of lactoperoxidase and horse-radish peroxidase are of the same order. The peroxidatic activity of lactoperoxidase is higher than that of horse-radish peroxidase. The mechanism of peroxidase action was interpreted as described by Chancet

A Gel-Chromatographic and Light Scattering Study of the Salmonella typhi Endotoxin

The endotoxin of Salmonella typhi, strain 0-901, was isolated by extraction with hypertonic (1 M) sodium chloride solutions and studied by gel-chromatography and light scattering methods. The gel-chromatographic separation was performed on Sepharose 2B, Sepharose 4B and Sephadex G-200 gels, and the fractionated material was monitored by ultraviolet and phenol-sulfuric acid colorimetry as well as by a photometric latex agglutination test. The extracted material consisted of two components: one was the high molecular weight endotoxin and the other a protein-polysaccharide complex of a molecular weight lower than 66,000 dalton. The light scattering experiments of endotoxin extracts showed the average molecular weights from 1.9 to 4.9 million dalton. The separation of the low molecular weight proteinic component was attempted by thermal denaturation, but this had to be abandoned owing to the denaturation and degradation of endotoxin. A high molecular weight endotoxin component was isolated by elution on a Sephadex G-200 column and had the molecular weight of 5.6 million dalton, which was in good accord with the value previously determined for a Boivin extraction sample. The high molecular weight endotoxin sample proved to be a highly polydispersed material. From the estimates of various averages of gyration radii it has been concluded that the particles of this sample have a more compact structurE than those of the Boivin extraction sample, possibly due to the tertiary structuring of polypeptide chains in the protein-lipopolysaccharide complex of the endotoxin particle