7,460 research outputs found
The Rapid and Sensitive Quantitative Determination of Galactose by Combined Enzymatic and Colorimetric Method: Application in Neonatal Screening
The quantitative measurement of galactose in blood is essential for the early diagnosis, treatment, and dietary monitoring of galactosemia patients. In this communication, we aimed to develop a rapid, sensitive, and cost-effective combined method for galactose determination in dry blood spots. This procedure was based on the combination of enzymatic reactions of galactose dehydrogenase (GalDH), dihydrolipoyl dehydrogenase (DLD), and alkaline phosphates with a colorimetric system. The incubation time and the concentration of enzymes used in new method were also optimized. The analytical performance was studied by the precision, recovery, linearity, and sensitivity parameters. Statistical analysis was applied to method comparison experiment. The regression equation and correlation coefficient (R2) were Y = 0.0085x + 0.032 and R2 = 0.998, respectively. This assay exhibited a recovery in the range of 91.7–114.3 % and had the limit detection of 0.5 mg/dl for galactose. The between-run coefficient of variation (CV) was between 2.6 and 11.1 . The within-run CV was between 4.9 and 9.2 . Our results indicated that the new and reference methods were in agreement because no significant biases exist between them. Briefly, a quick and reliable combined enzymatic and colorimetric assay was presented for application in newborn mass screening and monitoring of galactosemia patients. © 2016 Springer Science+Business Media New Yor
Dynamics of parametric fluctuations induced by quasiparticle tunneling in superconducting flux qubits
We present experiments on the dynamics of a two-state parametric fluctuator
in a superconducting flux qubit. In spectroscopic measurements, the fluctuator
manifests itself as a doublet line. When the qubit is excited in resonance with
one of the two doublet lines, the correlation of readout results exhibits an
exponential time decay which provides a measure of the fluctuator transition
rate. The rate increases with temperature in the interval 40 to 158 mK. Based
on the magnitude of the transition rate and the doublet line splitting we
conclude that the fluctuation is induced by quasiparticle tunneling. These
results demonstrate the importance of considering quasiparticles as a source of
decoherence in flux qubits.Comment: 12 pages, including supplementary informatio
Second Backbend in the Mass A ~ 180 Region
Within the framework of selfconsistent cranked Hartree-Fock- Bogoliubov
theory(one-dimensional) we predict second backbend in the yrast line of Os-182
at , which is even sharper than the first one observed
experimentally at .
Around such a high spin the structure becomes multi-quasiparticle type, but
the main source of this strong discontinuity is a sudden large alignment of
i_13/2 proton orbitals along the rotation axis followed soon by the alignment
of j_15/2 neutron orbitals. This leads to drastic structural changes at such
high spins. When experimentally confirmed, this will be observed for the first
time in this mass region, and will be at the highest spin so far.Comment: 13 pages, 4 ps figure
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