4 research outputs found
Lymphocyte Subpopulations in Pulmonary Tuberculosis Patients
Protection against Mycobacterium tuberculosis is based on cell-mediated immunity, most importantly involving CD4(+) and CD8(+) T-cell subsets. The aim of this study was to evaluate CD4(+) and CD8(+) T-cell profiles and CD19(+) and CD3(−)CD(16+56)(+) populations in patients with pulmonary tuberculosis. CD4(+) and CD8(+) T cells, B-lymphocytes, and natural killer (NK) cells were evaluated in 75 active (APTB) and 25 inactive (IPTB) pulmonary tuberculosis cases and 20 healthy subjects (HCs). The results were compared at different stages of antituberculosis treatment in the APTB patients and also according to X-ray findings in the newly diagnosed APTB patients. The percentages of CD4(+) T cells were significantly lower (P < .01) and those of CD3(−)CD(16 + 56)(+) cells were significantly higher (P < .01) in APTB patients than in HCs. CD8(+) T cells were significantly decreased (P < .05), and CD3(−)CD(16+56)(+) cells were significantly increased (P < .01), in IPTB patients compared to HCs. The percentages of CD4(+), CD8(+), CD3(−)CD19(+), and CD3(−)CD(16+56)(+) cells showed no differences at different times of the antituberculosis regimen, and different stages of newly diagnosed APTB patients. APTB patients have a reduced percentage of circulating CD4(+) T cells and an increased percentage of NK cells compared with healthy individuals. These cells could play important roles in the immune response to M tuberculosis infection
Changes in Serum Cytokine Levels in Active Tuberculosis With Treatment
It has been reported that IFN-γ, TNF-α, and IL-12
stimulate, and that IL-10, TGF-β, and IL-4 suppress the
protective immune response against tuberculosis. We aim
to evaluate changes in the serum levels of pro and
antiinflammatory cytokines in active pulmonary tuberculosis
(APTB) and the possible effects of treatment on these changes.
Serum IL-12p40, IL-4, IL-10, TNF-α, IFN-γ, and
TGF-β1 levels were determined in 20 APTB cases (group 1)
before and 2, 4, and 6 months after therapy. The same parameters
were also determined in 9 inactive pulmonary tuberculosis (IPTB)
cases (group 2) and 9 healthy controls (HC, group 3). Before
treatment, the mean serum IFN-γ, TNF-α, and IL-10
levels in group 1 were statistically higher than those in group 2
(P=.001, P=.024, P=.016, resp) or group 3 (P=.003, P=.002, P=.011, resp). The levels in group 1 decreased
significantly after treatment (P=.001 for IFN-γ,
P=.004
for TNF-α, P=.000 for IL-10). The serum levels
of IL-12p40 were significantly higher in group 1 than in group 3
(P=.012) and decreased insignificantly after treatment. There
was no difference in serum IL-4 and TGF-β1 levels among the
groups (P>.05). Because the serum IL-12p40, IL-10, TNF-α,
and IFN-γ levels were high in APTB, we believe that these
cytokines have important roles in the immune response to
Mycobacterium tuberculosis (M tuberculosis).
These parameters could be used in follow-up as indicators of the
success of APTB therapy