KlenTaq DNA Polymerase adopts unique recognition states when encountering matched, mismatched, and abasic template sites : an NMR Study

On the tracks of a DNA polymerase: NMR provides insights into DNA synthesis in a virtually label-free manner and under close-to-physiological conditions. Through the monitoring of the chemical-shift changes of multiple (13) C-methyl methionine residues we found unique recognition states for canonical and noncanonical cases, thus indicating enzymatic cycling through distinct paths