A Quantitative Chromatographic Method for the Determination of Leucine Aminopeptidase Activity

A micro method for the determination of leucine aminopeptidase activity was developed. Leucinamide was us ed as substrate. The liberated leucin e was quantitative ly determined using paper chromatography a nd reflectanc e-densitometrical scanning of the spots. From the spot area of leucine the enzyme activity was evaluated. This method is useful for the determination of leucine aminopeptidase activity in supernatants obtained after centrifugation of homogenized organs

A Quantitative Chromatographic Method for the Determination of Leucine Aminopeptidase Activity

A micro method for the determination of leucine aminopeptidase activity was developed. Leucinamide was us ed as substrate. The liberated leucin e was quantitative ly determined using paper chromatography a nd reflectanc e-densitometrical scanning of the spots. From the spot area of leucine the enzyme activity was evaluated. This method is useful for the determination of leucine aminopeptidase activity in supernatants obtained after centrifugation of homogenized organs