4 research outputs found
History and sensitivity comparison of the
The history of toxicity tests with duckweeds shows that these assays with free-floating
aquatic angiosperms are gaining increasing attention in ecotoxicological research and
applications. Standard tests have been published by national and international
organizations, mainly with the test species Lemna minor and Lemna
gibba. Besides the former two test species the great duckweed Spirodela
polyrhiza is to date also regularly used in duckweed testing. Under unfavorable
environmental conditions, the latter species produces dormant stages (turions) and this
has triggered the attention of two research groups from Belgium and Greece to jointly
develop a “stock culture independent” microbiotest with S. polyrhiza. A
72 h new test has been worked out which besides its independence of stock culturing and
maintenance of live stocks is very simple and practical to perform, and much less
demanding in space and time than the conventional duckweed tests. Extensive International
Interlaboratory Comparisons on the S. polyrhiza microbiotest showed its
robustness and reliability and triggered the decision to propose this new assay to the ISO
for endorsement and publication as a standard toxicity test for duckweeds. Sensitivity
comparison of the 72 h S. polyrhiza microbiotest with the 7d L.
minor assay for 22 compounds belonging to different groups of chemicals
revealed that based on growth as the effect criterion both duckweed assays have a similar
sensitivity. Taking into account its multiple advantages and assets, the S.
polyrhiza microbiotest is a reliable and attractive alternative to the
conventional duckweed tests
History and sensitivity comparison of the Spirodela polyrhiza
The history of toxicity tests with duckweeds shows that these assays with free-floating
aquatic angiosperms are gaining increasing attention in ecotoxicological research and
applications. Standard tests have been published by national and international
organizations, mainly with the test species Lemna minor and Lemna
gibba. Besides the former two test species the great duckweed Spirodela
polyrhiza is to date also regularly used in duckweed testing. Under unfavorable
environmental conditions, the latter species produces dormant stages (turions) and this
has triggered the attention of two research groups from Belgium and Greece to jointly
develop a “stock culture independent” microbiotest with S. polyrhiza. A
72 h new test has been worked out which besides its independence of stock culturing and
maintenance of live stocks is very simple and practical to perform, and much less
demanding in space and time than the conventional duckweed tests. Extensive International
Interlaboratory Comparisons on the S. polyrhiza microbiotest showed its
robustness and reliability and triggered the decision to propose this new assay to the ISO
for endorsement and publication as a standard toxicity test for duckweeds. Sensitivity
comparison of the 72 h S. polyrhiza microbiotest with the 7d L.
minor assay for 22 compounds belonging to different groups of chemicals
revealed that based on growth as the effect criterion both duckweed assays have a similar
sensitivity. Taking into account its multiple advantages and assets, the S.
polyrhiza microbiotest is a reliable and attractive alternative to the
conventional duckweed tests
History and sensitivity comparison of the Spirodela polyrhiza microbiotest and Lemna toxicity tests
The history of toxicity tests with duckweeds shows that these assays with free-floating
aquatic angiosperms are gaining increasing attention in ecotoxicological research and
applications. Standard tests have been published by national and international
organizations, mainly with the test species Lemna minor and Lemna
gibba. Besides the former two test species the great duckweed Spirodela
polyrhiza is to date also regularly used in duckweed testing. Under unfavorable
environmental conditions, the latter species produces dormant stages (turions) and this
has triggered the attention of two research groups from Belgium and Greece to jointly
develop a “stock culture independent” microbiotest with S. polyrhiza. A
72 h new test has been worked out which besides its independence of stock culturing and
maintenance of live stocks is very simple and practical to perform, and much less
demanding in space and time than the conventional duckweed tests. Extensive International
Interlaboratory Comparisons on the S. polyrhiza microbiotest showed its
robustness and reliability and triggered the decision to propose this new assay to the ISO
for endorsement and publication as a standard toxicity test for duckweeds. Sensitivity
comparison of the 72 h S. polyrhiza microbiotest with the 7d L.
minor assay for 22 compounds belonging to different groups of chemicals
revealed that based on growth as the effect criterion both duckweed assays have a similar
sensitivity. Taking into account its multiple advantages and assets, the S.
polyrhiza microbiotest is a reliable and attractive alternative to the
conventional duckweed tests