Introducing standardized field methods for fracture-focused surface process research

Rock fractures are a key contributor to a broad array of Earth surface processes due to their direct control on rock strength as well as rock porosity and permeability. However, to date, there has been no standardization for the quantification of rock fractures in surface process research. In this work, the case is made for standardization within fracture-focused research, and prior work is reviewed to identify various key datasets and methodologies. Then, a suite of standardized methods is presented as a starting “baseline” for fracture-based research in surface process studies. These methods have been shown in pre-existing work from structural geology, geotechnical engineering, and surface process disciplines to comprise best practices for the characterization of fractures in clasts and outcrops. This practical, accessible, and detailed guide can be readily employed across all fracture-focused weathering and geomorphology applications. The wide adoption of a baseline of data collected using the same methods will enable comparison and compilation of datasets among studies globally and will ultimately lead to a better understanding of the links and feedbacks between rock fracture and landscape evolution.</p

Dispersion as an Important Step in the Candida albicans Biofilm Developmental Cycle

Biofilms are dynamic microbial communities in which transitions between planktonic and sessile modes of growth occur interchangeably in response to different environmental cues. In the last decade, early events associated with C. albicans biofilm formation have received considerable attention. However, very little is known about C. albicans biofilm dispersion or the mechanisms and signals that trigger it. This is important because it is precisely C. albicans cells dispersed from biofilms that are the main culprits associated with candidemia and establishment of disseminated invasive disease, two of the gravest forms of candidiasis. Using a simple flow biofilm model recently developed by our group, we have performed initial investigations into the phenomenon of C. albicans biofilm dispersion, as well as the phenotypic characteristics associated with dispersed cells. Our results indicate that C. albicans biofilm dispersion is dependent on growing conditions, including carbon source and pH of the media used for biofilm development. C. albicans dispersed cells are mostly in the yeast form and display distinct phenotypic properties compared to their planktonic counterparts, including enhanced adherence, filamentation, biofilm formation and, perhaps most importantly, increased pathogenicity in a murine model of hematogenously disseminated candidiasis, thus indicating that dispersed cells are armed with a complete arsenal of “virulence factors” important for seeding and establishing new foci of infection. In addition, utilizing genetically engineered strains of C. albicans (tetO-UME6 and tetO-PES1) we demonstrate that C. albicans biofilm dispersion can be regulated by manipulating levels of expression of these key genes, further supporting the evidence for a strong link between biofilms and morphogenetic conversions at different stages of the C. albicans biofilm developmental cycle. Overall, our results offer novel and important insight into the phenomenon of C. albicans biofilm dispersion, a key part of the biofilm developmental cycle, and provide the basis for its more detailed analysis

Automated field detection of rock fracturing, microclimate, and diurnal rock temperature and strain fields

The rates and processes that lead to non-tectonic rock fracture on Earth's surface are widely debated but poorly understood. Few, if any, studies have made the direct observations of rock fracturing under natural conditions that are necessary to directly address this problem. An instrumentation design that enables concurrent high spatial and temporal monitoring resolution of (1) diurnal environmental conditions of a natural boulder and its surroundings in addition to (2) the fracturing of that boulder under natural full-sun exposure is described herein. The surface of a fluvially transported granite boulder was instrumented with (1) six acoustic emission (AE) sensors that record micro-crack associated, elastic wave-generated activity within the three-dimensional space of the boulder, (2) eight rectangular rosette foil strain gages to measure surface strain, (3) eight thermocouples to measure surface temperature, and (4) one surface moisture sensor. Additionally, a soil moisture probe and a full weather station that measures ambient temperature, relative humidity, wind speed, wind direction, barometric pressure, insolation, and precipitation were installed adjacent to the test boulder. AE activity was continuously monitored by one logger while all other variables were acquired by a separate logger every 60 s. The protocols associated with the instrumentation, data acquisition, and analysis are discussed in detail. During the first four months, the deployed boulder experienced almost 12 000 AE events, the majority of which occur in the afternoon when temperatures are decreasing. This paper presents preliminary data that illustrates data validity and typical patterns and behaviors observed. This system offers the potential to (1) obtain an unprecedented record of the natural conditions under which rocks fracture and (2) decipher the mechanical processes that lead to rock fracture at a variety of temporal scales under a range of natural conditions

Dating fractures using luminescence

Rock fracturing (cracking) is a universal process that drives and limits chemical degradation, sediment production and erosion, and deterioration of infrastructure. Despite extensive research gains in rock mechanics on one hand and geochronology on the other, there remains a glaring gap in our ability to understand the long term evolution of natural, in situ fractures. Here we develop a novel fracture exposure dating technique, grounded in modern advances in luminescence geochronology. We apply our new dating method to a granitic boulder from a glacial outwash terrace in California, US. We conclude that the longest, clast-splitting E-W fracture appeared shortly after the boulder's deposit, whereas the secondary N-S fracture appeared 5 ka after the deposition, approximately correlating with the Last Glacial Maximum and Younger Dryas periods of the region, respectively. However, dating of the third fracture (&lt;&lt; 50 µm width) which does not fully split the rock, is ambiguous due to negligible daylight penetration and poor determination of fracture width. The fracture dating method presented herein brings with it the potential to decipher relationships that are crucial for the interpretation and modeling of, for example, long-term landscape and atmospheric evolution relating rock weathering to climate change and erosion.</p

Multimethodological study of non-linear strain effects induced by thermal stresses on jointed rock masses

A multimethodological method based on environmental, stress–strain, microseismic, and ambient seismic noise monitoring is here presented, with a view to identifying non-linearity of thermally-induced deformation of jointed rock masses at different dimensional scales. Rock masses experience non-negligible deformation cycles due to the continuous fluctuations of their surficial temperatures. However, the interpretation of such strain effects, in terms of the ratio between elastic and inelastic percentages, is still debated. In particular, the relation between microseismic emissions, considered as primary indicators of crack-growth related energy release, and resonant frequencies fluctuations of rock structures, witnesses of the thermally-induced effect at the macro- or structure-scale, have not been yet studied within a coupled framework. The combination of different approaches able to investigate the behavior of rock masses from micro- to macro-scale, then from fracture-scale to joint-isolated rock blocks up to rock structures, could provide new insights and perspectives on the effects related to shallow thermal stresses fluctuations. This paper presents the preliminary outcomes from two case studies, the Acuto experimental test-site (Italy) and the Wied Il-Mielaħ sea arch (Malta), where multiparametric monitoring surveys were conducted and are still ongoing, aiming at the assessment of the cause-to-effect relation between near-surface thermal stresses and induced strains. Data analysis was carried out following different approaches, with a particular emphasis on the Acuto test-site dataset recorded so far, allowing to establish a well-constrained correlation among temperature fluctuations and rock mass deformation both at the daily and seasonal scale