Tyrosine kinase inhibitor therapy-induced changes in humoral immunity in patients with chronic myeloid leukemia

Purpose Tyrosine kinase inhibitors (TKIs) have well-characterized immunomodulatory effects on T and NK cells, but the effects on the humoral immunity are less well known. In this project, we studied TKI-induced changes in B cell-mediated immunity. Methods We collected peripheral blood (PB) and bone marrow (BM) samples from chronic myeloid leukemia (CML) patients before and during first-line imatinib (n = 20), dasatinib (n = 16), nilotinib (n = 8), and bosutinib (n = 12) treatment. Plasma immunoglobulin levels were measured, and different B cell populations in PB and BM were analyzed with flow cytometry. Results Imatinib treatment decreased plasma IgA and IgG levels, while dasatinib reduced IgM levels. At diagnosis, the proportion of patients with IgA, IgG, and IgM levels below the lower limit of normal (LLN) was 0, 11, and 6% of all CML patients, respectively, whereas at 12 months timepoint the proportions were 6% (p = 0.13), 31% (p = 0.042) and 28% (p = 0.0078). Lower initial Ig levels predisposed to the development of hypogammaglobulinemia during TKI therapy. Decreased Ig levels in imatinibtreated patients were associated with higher percentages of immature BM B cells. The patients, who had low Ig levels during the TKI therapy, had significantly more frequent minor infections during the follow-up compared with the patients with normal Ig values (33% vs. 3%, p = 0.0016). No severe infections were reported, except recurrent upper respiratory tract infections in one imatinib-treated patient, who developed severe hypogammaglobulinemia. Conclusions TKI treatment decreases plasma Ig levels, which should be measured in patients with recurrent infections.Peer reviewe

Constituents of plants growing in Qatar - Part XIX. Flavonoids of Glossonema edule N. E. Br.

The study of the flavonoids of the unripe fruits of Glossonema edule, resulted in the isolation and identification of apigenin 6,8-di-C-glucoside (violanthin) and luteolin 7-O-glucoside. © 1990 Kluwer Academic Publishers

مكونات نباتات دوله قطر : الجزء 24 الفحص الكيميائي لنبات السالفيا (نعيم)

The study of the flavonoids revealed the isolation and identification of luteolin, luteolin 7-O- glucoside, apigenin and apigenin 7-0 glucoside; their identity was proved by m.p., TLC, UV, IR and MS. Moreover, a coumarin was isolated and identified as herniarin. Investigation of the lipid fraction resulted in the isolation and identification of a and P amyrins. The fatty acids were identified as undecanoic, dodecanoic, tridecanoic, myristic, pentadecanoic, palmitic, heptadecanoic, stearic, oleic and linoleic acids.أسفرت دراسة الفلافونيدات عن فصل والتعرف على المركبات الاَتية : ليتيولين ، ليتيولين - 7 - جليكوزيد ، أبيجنين ، أ بيجنين - 7 - جليكوزيد . كما اسفرت دراسة الجزء الدهني للنبات عن فصل والتعرف على ألفا، بيتا - أميرين . وتم التعرف على الأحماض الدهنية

دراسة كيميائية لنبات الثيمس ديكساتس (الزعتر) 1- الفلافونيدات و الزيوت الطيارة

Investigation of the flavonoidal constituents of Thymus decassatus, L. resulted in the isolation and identification of thymonin, 5,6,4'-trihydroxy-7,3'-dimethoxyl flavone, and apigenin. Investigation of the volatile oil by GC revealed the identification of 41 components. Moreover, thymol as the major component was isolated from the volatile oil fraction.أسفرت .دراسة الفلافونيدات لنبات الزعتر عن فصل وتعريف كل من الثيمونين 5 ، 6 ، 4 - تراى هيدروكسي -7 - 3 ، داى ميثوكسي فلافون وكذلك الابيجينين . وأظهرت دراسة الزيت الطيار بواسطة كروماتوجرافيا الغاز عن التعرف على 41 مركب متضمنة مادة الثيمول الذي تم فصلها من جزء الزيت الطيار

Human Papilloma Virus (HPV) in Egyptian women: Molecular diagnosis and genotyping

No Abstract

Isolation and Characterization of Antineoplastic Alkaloids from Catharanthus Roseus L. Don. Cultivated in Egypt

Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC), Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone)

Lipophilic Constituents of Rumex vesicarius L. and Rumex dentatus L.

Rumex dentatus L. and Rumex vesicarius L., of the family Polygonaceae, are edible herbs growing wild in Egypt. Their lipoid constituents were examined by both liquid chromatography/mass spectrometry (LC/MS) and by gas chromatography/mass spectrometry (GC/MS). Their essential oil compositions consisted mainly of thujene, limonene, fenchon, estragole, and anethole but at largely different concentration. Fatty acid compositions were similar among the two species and consisting of palmitic, oleic, linoleic and linolenic acids, with R. vesicarius containing much higher level of omega-3-fatty acids. Both of the crude lipid extracts of the two species showed strong antioxidant activity as a radical quenching agent against 2,2-diphenyl-1-picrylhydrazyl (DPPH) systems. Antioxidant activities were mostly associated with the polar lipid fractions. High performance thin layer chromatography (HPTLC), both in the normal and reversed phase,as well as liquid chromatography/mass spectrometry (LC/MS) in the positive and negative electrospray ionization (ESI), showed unique chemical profile for each species that can be useful for species identification and quality control of herbal drug formulations. R. vesicarius was characterized by abundances of flavonoids and R. dentatus was abundant in anthraquinones and chromones