Growth Characteristics and Ergosterol Content of Grifola frondosa in Various Solid-state Substrates

Growth characteristics of medicinal mushroom Grifola frondosa mycelia were studied in solid-state cultivation on various solid-state substrates in different setups. The mycelial growth rate was determined in racing tubes by the measurement of ergosterol content. The fastest growth rate of 3.76 mm d–1 and the highest biomass amount of 54 mg g–1 were achieved. The results were scaled-up in a horizontal stirred bioreactor, where higher amounts of biomass (53.2 mg g–1 in 42 days) were achieved. These results represent an available platform for large-scale production of medicinal fungi biomass in bioreactors