Appropriate end points for the characterization of behavioral changes in developmental toxicology.

The present paper is devoted to second- and higher-tier test methods for the characterization of behavioral changes produced in rodents by exposure to noxious agents during development. The paper analyzes a series of end points that are informative about specific processes and underlying regulatory mechanisms but require greater technical sophistication and larger investments than first-tier end points. This applies to ultrasonic emissions in successive postnatal periods; to mother-pup interactions, including appropriate cross-fostering controls; to social (including sexual) interaction tests from the infantile to the young adult stage; and to a variety of conditioning and learning tests using both positive and negative reinforcement

Estimating flooded area and mean water level using active and passive microwaves: the example of Paraná River Delta floodplain

This paper describes a procedure to estimate both the fraction of flooded area and the mean water level in vegetated river floodplains by using a synergy of active and passive microwave signatures. In particular, C band Envisat ASAR in Wide Swath mode and AMSR-E at X, Ku and Ka band, are used. The method, which is an extension of previously developed algorithms based on passive data, exploits also model simulations of vegetation emissivity. The procedure is applied to a long flood event which occurred in the Paraná River Delta from December 2009 to April 2010. Obtained results are consistent with in situ measurements of river water level

In Vitro Evaluation of the Antibacterial Activity of the Peptide Fractions Extracted from the Hemolymph of Hermetia illucens (Diptera: Stratiomyidae)

Antimicrobial peptides (AMPs) are a chemically and structurally heterogeneous family of molecules produced by a large variety of living organisms, whose expression is predominant in the sites most exposed to microbial invasion. One of the richest natural sources of AMPs is insects which, over the course of their very long evolutionary history, have adapted to numerous and different habitats by developing a powerful innate immune system that has allowed them to survive but also to assert themselves in the new environment. Recently, due to the increase in antibiotic-resistant bacterial strains, interest in AMPs has risen. In this work, we detected AMPs in the hemolymph of Hermetia illucens (Diptera, Stratiomyidae) larvae, following infection with Escherichia coli (Gram negative) or Micrococcus flavus (Gram positive) and from uninfected larvae. Peptide component, isolated via organic solvent precipitation, was analyzed by microbiological techniques. Subsequent mass spectrometry analysis allowed us to specifically identify peptides expressed in basal condition and peptides differentially expressed after bacterial challenge. We identified 33 AMPs in all the analyzed samples, of which 13 are specifically stimulated by Gram negative and/or Gram positive bacterial challenge. AMPs mostly expressed after bacterial challenge could be responsible for a more specific activity

TIMES-EU: a Pan-European model integrating LCA and external costs

n/

Working in (Slow) Progress: Socio-Environmental and Economic Dynamics in the Forestry Sector and the Contribution to Sustainable Development in Europe

Forest ecosystems are increasingly subject to disturbances, such as extreme (climate) events, fires and pathological outbreaks, which exert significant (and still poorly quantified) economic impacts, despite their intrinsic resilience. How forest management addresses these challenges will have profound effects on human health, environmental diversity, (ecological and economic) productivity and the ability of forest ecosystems to recovery from exogenous shocks. Assuming forests as ensuring ecosystem services that are vital to society and human well-being, in addition to providing wood material, a better knowledge of forest ecosystems appears a key requirement to delineate a developmental strategy that guarantees environmental protection objectives and achievement of climate and energy targets. In the context of Sustainable Development Goals of the United Nations 2030 Agenda, the information available on forest management practices in Europe still seems not completely suitable to provide a comprehensive overview of ecosystem conditions, their economic value, and their biodiversity. Based on a literature review, the present contribution focuses on recent dynamics of the forestry sector in Europe, delineating the inherent complexity in the environmental-economic nexus. Additionally, the extensive use of a key natural resource such as wood exerts social implications for local districts adapting to ecological change-from climate warming to landscape transformations. In line with earlier studies, our paper confirms the role of the technical-economic dimension of forestry in sustainable development paths of districts and regions, affecting together (i) short-term economic dynamics, (ii) the growth prospects of the sector, (iii) the organization of the supply chain, (iv) the interconnections between companies, and (v) investment strategies at large. One of the major issues is the low reliability of official statistics to provide a coherent picture of the actual status of forest resources. At the same time, forestry practices are pivotal in maintaining habitats and species while increasing the timber production that remains (too) sustainable. This is the policy direction constantly given by the European Commission to stimulate over time public bodies and private (in whose hands is about 60% of European forests) to start virtuous paths in the circular economy with considerable repercussions in terms of additional jobs, recycled materials, mt of CO2 equivalent avoided, and increase in community added value. In this context, forestry should assure a more 'holistic' contribution to sustainable development paths at various spatial scales. This means concentrating on both economic and environmental targets based on the identification of significant (ecological-economic) dimensions that may delineate future lines of investigation and policy intervention, while increasing between member countries and regional authorities

An assessment of attitudes towards plastics and bioplastics in Europe

Over the last 50 years, conventional fossil-based plastics have become an integral part of our everyday lives. Apart from their low production costs, this is due to a number of their unique properties, including durability, strength, lightness, electrical and thermal insulation, resistance to chemicals and corrosion. The production of plastics has increased from 1.5 million metric tons in 1950 to 359 million metric tons in 2018. Of this total, 61.8 million metric tons were produced in Europe. There are various problems associated with plastic use and disposal that pose a serious threat to both the physical environment and human health. Since public behaviour plays a key role when it comes to the use of plastic, this paper reports on a study that focused on an assessment of attitudes towards plastics and bioplastics in Europe. The results showed that packaging is the most frequent modality of plastic used among participants. In addition, majority of participants are aware that plastic waste can affect environment and human health and therefore segregate and properly dispose plastics. Also, even though most respondents were aware of the environmental problems related to plastic use and showed a positive inclination towards using bioplastic materials, their limited availability and lack of relevant information about bioplastics pose a problem for wider use. Departing from the assumption that the public attitude is a determining factor in the consumption of plastics as a whole and bioplastics in particular, this paper also sheds some light on the current situation, identifying some trends and information gaps which should be addressed in order to encourage a more rational use of plastics in Europe

Dedicated versus mainstreaming approaches in local climate plans in Europe

Cities are gaining prominence committing to respond to the threat of climate change, e.g., by developing local climate plans or strategies. However, little is known regarding the approaches and processes of plan development and implementation, or the success and effectiveness of proposed measures. Mainstreaming is regarded as one approach associated with (implementation) success, but the extent of integration of local climate policies and plans in ongoing sectoral and/or development planning is unclear. This paper analyses 885 cities across the 28 European countries to create a first reference baseline on the degree of climate mainstreaming in local climate plans. This will help to compare the benefits of mainstreaming versus dedicated climate plans, looking at policy effectiveness and ultimately delivery of much needed climate change efforts at the city level. All core cities of the European Urban Audit sample were analyzed, and their local climate plans classified as dedicated or mainstreamed in other local policy initiatives. It was found that the degree of mainstreaming is low for mitigation (9% of reviewed cities; 12% of the identified plans) and somewhat higher for adaptation (10% of cities; 29% of plans). In particular horizontal mainstreaming is a major effort for local authorities; an effort that does not necessarily pay off in terms of success of action implementation. This study concludes that climate change issues in local municipalities are best tackled by either, developing a dedicated local climate plan in parallel to a mainstreamed plan or by subsequently developing first the dedicated and later a mainstreaming plan (joint or subsequent “dual track approach”). Cities that currently provide dedicated local climate plans (66% of cities for mitigation; 26% of cities for adaptation) may follow-up with a mainstreaming approach. This promises effective implementation of tangible climate actions as well as subsequent diffusion of climate issues into other local sector policies. The development of only broad sustainability or resilience strategies is seen as critical.We thank the many council representatives that supported the datacollection. Special thanks to Birgit Georgi who helped in setting up this large net work of researchers across the EU-28. We also thank the EU COST Action TU 0902 (ledbyRichardDawson) that established the core research network and the positive engagement and interaction of th emembers of this group. OH is Fellow of the Tyndall Centre for Climate Change Research and was funded by the UK EPSRC LC Transforms: Low Carbon Transitions of Fleet Operations in Metropolitan Sites Project (grant number EP/N010612/1). EKL was supported by the Ministry of Education, Youth and Sports, Czechia, within the National Sustainability Program I (NPU I) (grant number LO1415). DG ac-knowledges support by the Ministry of Education, University and Research (MIUR), Italy ("Departments of Excellence" grant L. 232/2016). HO was supported by the Ministry of Education and Research, Estonia (grantnumberIUT34-17). MO acknowledges funding from the Ministry of Economy and Competitiveness (MINECO), Spain (grant number IJCI-2016-28835). SS acknowledges that CENSE's research is partially funded by the Science Foundation, Portugal (grant number UID/AMB/04085/2019). The paper reflects only the views of the authors. The European Union, the European Environment Agency or other supporting bodies are not liable for any use that may be made of the information that is provided in this manuscript