206 research outputs found
Thermodynamics of nicotinamide-adenine dinucleotide addition to the glyceraldehyde 3-phosphate dehydrogenases of yeast and of rabbit skeletal muscle. An equilibrium and calorimetric analysis over a range of temperatures
Studies on the Mechanism of Action of Glyceraldehyde 3-Phosphate Dehydrogenase. Absorbance and Fluorescence Properties of Reduced Nicotinamide-Adenine Dinucleotide Complexes with Glyceraldehyde 3-Phosphate Dehydrogenase *
Induced expression of microsomal cytochrome b<sub>5</sub> determined at mRNA and protein levels in rats exposed to ellipticine, benzo[a]pyrene, and 1-phenylazo-2-naphthol (Sudan I)
ABSTRACT: The microsomal protein cytochrome b (5), which is located in the membrane of the endoplasmic reticulum, has been shown to modulate many reactions catalyzed by cytochrome P450 (CYP) enzymes. We investigated the influence of exposure to the anticancer drug ellipticine and to two environmental carcinogens, benzo[a]pyrene (BaP) and 1-phenylazo-2-naphthol (Sudan I), on the expression of cytochrome b (5) in livers of rats, both at the mRNA and protein levels. We also studied the effects of these compounds on their own metabolism and the formation of DNA adducts generated by their activation metabolite(s) in vitro. The relative amounts of cytochrome b (5) mRNA, measured by real-time polymerase chain reaction analysis, were induced by the test compounds up to 11.7-fold in rat livers. Western blotting using antibodies raised against cytochrome b (5) showed that protein expression was induced by up to sevenfold in livers of treated rats. Microsomes isolated from livers of exposed rats catalyzed the oxidation of ellipticine, BaP, and Sudan I and the formation of DNA adducts generated by their reactive metabolite(s) more effectively than hepatic microsomes isolated from control rats. All test compounds are known to induce CYP1A1. This induction is one of the reasons responsible for increased oxidation of these xenobiotics by microsomes. However, induction of cytochrome b (5) can also contribute to their enhanced metabolism. GRAPHICAL ABSTRACT: [Image: see text
Interaction of nicotinamide adenine dinucleotide and its analogs with glyceraldehyde 3-phosphate dehydrogenase
Using Florida's Adult Protective Services Data in Research: Opportunities and Challenges
Spectral and Time-Resolved Analysis of Light-Induced Changes of Nadph Fluorescence in Chloroplasts
A new instrument for investigation of autofluorescence of the human body surface and for fluorometric determination of in vivo skin extracts (skinspectrofluorometer)
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