Frizzled-8 integrates Wnt-11 and transforming growth factor-β signaling in prostate cancer

Wnt-11 promotes cancer cell migration and invasion independently of β-catenin but the receptors involved remain unknown. Here, we provide evidence that FZD8 is a major Wnt-11 receptor in prostate cancer that integrates Wnt-11 and TGF-β signals to promote EMT. FZD8 mRNA is upregulated in multiple prostate cancer datasets and in metastatic cancer cell lines in vitro and in vivo. Analysis of patient samples reveals increased levels of FZD8 in cancer, correlating with Wnt-11. FZD8 co-localizes and co-immunoprecipitates with Wnt-11 and potentiates Wnt-11 activation of ATF2-dependent transcription. FZD8 silencing reduces prostate cancer cell migration, invasion, three-dimensional (3D) organotypic cell growth, expression of EMT-related genes, and TGF-β/Smad-dependent signaling. Mechanistically, FZD8 forms a TGF-β-regulated complex with TGF-β receptors that is mediated by the extracellular domains of FZD8 and TGFBR1. Targeting FZD8 may therefore inhibit aberrant activation of both Wnt and TGF-β signals in prostate cancer

Exploring DCO+^+ as a tracer of thermal inversion in the disk around the Herbig Ae star HD163296

We aim to reproduce the DCO$^+$ emission in the disk around HD163296 using a simple 2D chemical model for the formation of DCO$^+$ through the cold deuteration channel and a parametric treatment of the warm deuteration channel. We use data from ALMA in band 6 to obtain a resolved spectral imaging data cube of the DCO$^+$ $J$=3--2 line in HD163296 with a synthesized beam of 0."53$\times$ 0."42. We adopt a physical structure of the disk from the literature that reproduces the spectral energy distribution. We then apply a simplified chemical network for the formation of DCO$^+$ that uses the physical structure of the disk as parameters along with a CO abundance profile, a constant HD abundance and a constant ionization rate. Finally, from the resulting DCO$^+$ abundances, we calculate the non-LTE emission using the 3D radiative transfer code LIME. The observed DCO$^+$ emission is reproduced by a model with cold deuteration producing abundances up to $1.6\times 10^{-11}$. Warm deuteration, at a constant abundance of $3.2\times 10^{-12}$, becomes fully effective below 32 K and tapers off at higher temperatures, reproducing the lack of DCO$^+$ inside 90 AU. Throughout the DCO$^+$ emitting zone a CO abundance of $2\times 10^{-7}$ is found, with $\sim$99\% of it frozen out below 19 K. At radii where both cold and warm deuteration are active, warm deuteration contributes up to 20\% of DCO$^+$, consistent with detailed chemical models. The decrease of DCO$^+$ at large radii is attributed to a temperature inversion at 250 AU, which raises temperatures above values where cold deuteration operates. Increased photodesorption may also limit the radial extent of DCO$^+$. The corresponding return of the DCO$^+$ layer to the midplane, together with a radially increasing ionization fraction, reproduces the local DCO$^+$ emission maximum at $\sim$260 AU.Comment: 9 pages, 5 figures, accepted 7th July 201

Silver nanoparticles–polyethyleneimine-based coatings with antiviral activity against SARS-CoV-2: a new method to functionalize filtration media

The use of face masks and air purification systems has been key to curbing the transmission of SARS-CoV-2 aerosols in the context of the current COVID-19 pandemic. However, some masks or air conditioning filtration systems are designed to remove large airborne particles or bacteria from the air, being limited their effectiveness against SARS-CoV-2. Continuous research has been aimed at improving the performance of filter materials through nanotechnology. This article presents a new low-cost method based on electrostatic forces and coordination complex formation to generate antiviral coatings on filter materials using silver nanoparticles and polyethyleneimine. Initially, the AgNPs synthesis procedure was optimized until reaching a particle size of 6.2 ± 2.6 nm, promoting a fast ionic silver release due to its reduced size, obtaining a stable colloid over time and having reduced size polydispersity. The stability of the binding of the AgNPs to the fibers was corroborated using polypropylene, polyester-viscose, and polypropylene-glass spunbond mats as substrates, obtaining very low amounts of detached AgNPs in all cases. Under simulated operational conditions, a material loss less than 1% of nanostructured silver was measured. SEM micrographs demonstrated high silver distribution homogeneity on the polymer fibers. The antiviral coatings were tested against SARS-CoV-2, obtaining inactivation yields greater than 99.9%. We believe our results will be beneficial in the fight against the current COVID-19 pandemic and in controlling other infectious airborne pathogens. © 2022 by the authors. Licensee MDPI, Basel, Switzerland

Use and effectiveness of fly goalkeepers in european futsal

Using a fly goalkeeper (FG) is one of futsal´s most specific offensive strategy and gives leverage over the opponent to change the game''s final result. This study will analyze the goals obtained from the use of a FG and relate them to the score momentum variable and others in order to offer a better understanding and to establish if there are differences between main European futsal leagues. Sample made from all offensive situations that lead to a goal while using FG scheme (n=673) during 2014-2015 Spanish, Russian and Italian pro futsal leagues. Observational, nomothetic and multidimensional study. Statistical analysis using the SPSS vr 22 for inferential and descriptive statistics. Chi-square relation for cathegorical variables and Spearman''s Rho to establish non parametrical bi-varial correlations for ordinary variables, establishing significant differences p <0.05. FG strategy obtains 15.33% of the total goals. The league''s behaviour very similar, except in the Italian league, which scores more goals with their attack than against its defence, making differences between goals scored as local and visitor both in attack and defence

Co-pyrolysis of sewage sludge and manure

The management and valorization of residual organic matter, such as sewage sludge and manure, is gaining interest because of the increasing volume of these residues, their localized generation and the related problems. The anaerobic digestion of mixtures of sewage sludge and manure could be performed due to the similarities between both residues. The purpose of this study is to evaluate the feasibility of the co-pyrolysis of sewage sludge (SS) and digested manure (DM) as a potential management technology for these residues. Pyrolysis of a sewage sludge/manure blend (50:50%) was performed at 525 °C in a stirred batch reactor under N2 atmosphere. The product yields and some characteristics of the product were analyzed and compared to the results obtained in the pyrolysis of pure residues. Potential synergetic and antagonist effects during the co-pyrolysis process were evaluated. Although sewage sludge and manure seem similar in nature, there are differences in their pyrolysis product properties and distribution due to their distinct ash and organic matter composition. For the co-pyrolysis of SS and DM, the product yields did not show noticeable synergistic effects with the exception of the yields of organic compounds, being slightly higher than the predicted average, and the H2 yield, being lower than expected. Co-pyrolysis of SS and DM could be a feasible management alternative for these residues in locations where both residues are generated, since the benefits and the drawbacks of the co-pyrolysis are similar to those of the pyrolysis of each residue

A literatura das receitas comerciais em aeroportos: discuss\~oes e principais descobertas

The exploration of existing commercial opportunities has been increasing the share of commercial (non-aeronautical) revenues in the total revenues of airports worldwide. These revenues, also called commercial, non-aeronautical, or non-tariff revenues, come from rentals, duty-free shops, food and beverage sales, parking, advertising, etc. In other words, everything that is not the main business of the airport. Consequently, the dependence on commercial revenues has also become increasingly important, and airport managers are interested in understanding how to improve their financial results with this new revenue source, as it not only improves financial outcomes but also optimizes passengers' time and money consumption options at airports. Therefore, this chapter seeks to discuss the main determinants of commercial revenues at national airports, analyzing the possible impacts of the behavior of passengers from low-cost carriers (LCC) on these revenues, combined with other determining factors.Comment: This article is written in Portugues

Neurorrestauración tras la isquemia cerebral: papel de los neurotransmisores en la neurogénesis postisquémica

Introduction. Brain ischemia and reperfusion produce alterations in the microenvironment of the parenchyma, including ATP depletion, ionic homeostasis alterations, inflammation, release of multiple cytokines and abnormal release of neurotransmitters. As a consequence, the induction of proliferation and migration of neural stem cells towards the peri-infarct region occurs. Development. The success of new neurorestorative treatments for damaged brain implies the need to know, with greater accuracy, the mechanisms in charge of regulating adult neurogenesis, both under physiological and pathological conditions. Recent evidence demonstrates that many neurotransmitters, glutamate in particular, control the subventricular zone, thus being part of the complex signalling network that influences the production of new neurons. Conclusion. Neurotransmitters provide a link between brain activity and subventricular zone neurogenesis. Therefore, a deeper knowledge of the role of neurotransmitters systems, such as glutamate and its transporters, in adult neurogenesis, may provide a valuable tool to be used as a neurorestorative therapy in this pathology.Peer Reviewe