3 research outputs found
LâacĂ©rola, un fruit exotique riche en vitamine C contient une LTP allergĂ©nique
International audienceAim of the studyAcerola is a tropical fruit 20 to 30 times richer in vitamin C than oranges. Acerola is found in tablets or powders commercially available for their richness in vitamin C and also in the form of juice or as additives in compotes and fruit juices. Besides a previous report on the existence of an IgE cross-reactivity of an unidentified acerola protein with Hev b 6 from latex no studies on acerola allergens have been performed.Patients and methodsAcerola proteins were extracted from fresh fruit, commercial juice and a vitamin C-enriched powder. Allergens were studied by an allergomics analysis, including IgE immunoblots, using sera from documented patients followed by protein identification using mass spectrometry.ResultsIgE Immunoblots performed with patients sensitized to LTPs, showed IgE reactivities in low molecular mass corresponding to LTPs. In contrast, no allergenic gibberellin-regulated protein (GRP), PR10 and profilin were revealed. The presence of LTP was confirmed by the use of specific anti-Pru p 3 antibodies and their identification in fresh fruit and commercial juice extracts by mass spectrometry that further unravelled several putative allergens. LTP was also detected in vitamin C-enriched powder.ConclusionAcerola fruit and juice as well as derived products containing acerola carries a risk for LTP sensitized patients.But de lâĂ©tudeLâacĂ©rola est un fruit tropical 20 Ă 30 fois plus riche en vitamine C que lâorange. On trouve de lâacĂ©rola dans des comprimĂ©s ou des poudres vendus pour leur richesse en vitamine C et aussi sous forme de jus ou en additif dans des compotes ou jus de fruit. ExceptĂ© la mise en Ă©vidence dâune rĂ©activitĂ© croisĂ©e dâune protĂ©ine non identifiĂ©e dâacĂ©rola avec Hev b 6 du latex, les allergĂšnes de lâacĂ©rola nâont pas Ă©tĂ© Ă©tudiĂ©s.Patients et mĂ©thodesLes protĂ©ines dâacĂ©rola ont Ă©tĂ© extraites Ă partir du fruit frais, dâun jus commercial et de poudre riche en vitamine C. Les allergĂšnes ont fait lâobjet dâune analyse allergomique incluant des immunoempreintes grĂące Ă des patients documentĂ©s et lâidentification des protĂ©ines par spectromĂ©trie de masse.RĂ©sultatsLes immunoempreintes IgE rĂ©alisĂ©es avec des patients sensibilisĂ©s aux lipid transfer protein (LTP) prĂ©sentent une rĂ©activitĂ© IgE dans les faibles masses molĂ©culaires correspondant aux LTP. En revanche, ni gibberellin-regulated protein (GRP), ni PR10 et ni profiline allergĂ©niques ne sont rĂ©vĂ©lĂ©es. La prĂ©sence de LTP est confirmĂ©e par lâutilisation dâanticorps anti-LTP de pĂȘche et par leur identification dans les extraits de fruit frais et de jus commercial par spectromĂ©trie de masse qui rĂ©vĂšle, en outre, de nombreux allergĂšnes potentiels. On peut aussi dĂ©tecter la prĂ©sence de LTP dans la poudre riche en vitamine C.ConclusionLe jus dâacĂ©rola et les produits dĂ©rivĂ©s contenant de lâacĂ©rola comportent donc un risque certain pour les patients sensibilisĂ©s aux LTP