USE OF BIO-ENZYMATIC PREPARATIONS FOR ENHANCEMENT BIOGAS PRODUCTION

Abstract VÍTĚZ, T., HAITL, M., KARAFIÁT, Z., MACH, P., FRYČ, J., LOŠÁK, T., SZOSTKOVÁ, M.: Use of bioenzymatic preparations for enhancement biogas production. Acta univ. agric. et silvic. Mendel. Brun., 2011, LIX, No. 3, pp. 203-208 Biogas is a renewable energy resource with high increasing developed in last few decades. It's big opportunity for stabilization rural areas, concretely agriculture sector. This technology can decentralize supply of energy. The number of operated biogas plants is rapidly increasing. Biogas plants require a high level of intensity and stableness of the process of anaerobic fermentation with biogas production for effi ciency treatment, also for good quality of development biogas and fertilization eff ect of the rest of fermentation. If this is not completed the operator has problem to keep the process in optimal condition for anaerobic fermentation. Researchers have tried diff erent techniques to enhance biogas production. In order to achieve the aforementioned state, it is essential to ensure increased activity of microorganisms that contribute to the anaerobic fermentation. The metabolic activity of microorganisms is preconditioned by availability of easily decomposable solids. Adding of bacterial and enzymatic cultures into a fermented substrate represents one of the possibilities. The enzymes contained in this preparation are responsible for better exposing methanogenic bacteria to the material. The tested bio-enzymatic preparation, APD BIO GAS, is a mixture that contains bacteria and enzymes which are essential for the effi cient progress of anaerobic fermentation. The reference biogas laboratory of the Mendel University in Brno was used for the purpose of testing of APD BIOGAS in mesophilic conditions of anaerobic fermentation on a substrate consisting of a mixture of maize silage and liquid manure. The producer of this preparation declare enhancement of quality and quantity of developed biogas, elimination of smell level of the rest of fermentation its higher homogenity. For the test were used lab scale fermenters of batch type with work volume 0.12 m 3 . An increase of biogas production by 15% was determined in connection with addition of the preparation, also with higher decrease of total solids and decrease of organic substance in total solids in the fermenter where were used this preparation. biogas production, anaerobic fermentation, enzymatic preparations Functionality of our society is currently dependent on exploiting of the limited amount of non-renewable resources of energy. The everincreasing energy demands force us to look for a replacement of the non-renewable resources of energy by other (renewable) resources. One of the available alternatives pertains to use of biomass within the process of anaerobic fermentation resulting in creation of biogas that may be further used for energy-related purposes. Anaerobic technologies off er an attractive manner of use of biomass resources for the purpose of partial satisfying of the energy needs of our society Anaerobic fermentation is a process typical for decomposition of organic matter without in an environment in which air is not present. It consists of four stages: hydrolysis, acidogenesis, acetogenesis and methanogenesis. In the course of individual stages high-molecular substances such as fat, carbohydrates, proteins, nucleic acids, etc., are decomposed into low-molecular substances including their subsequent transformation into the acetic acid, carbon dioxide and hydrogen

Use of bio-enzymatic preparations for enhancement biogas production

Biogas is a renewable energy resource with high increasing developed in last few decades. It’s big opportunity for stabilization rural areas, concretely agriculture sector. This technology can decentralize supply of energy. The number of operated biogas plants is rapidly increasing. Biogas plants require a high level of intensity and stableness of the process of anaerobic fermentation with biogas production for efficiency treatment, also for good quality of development biogas and fertilization effect of the rest of fermentation. If this is not completed the operator has problem to keep the process in optimal condition for anaerobic fermentation. Researchers have tried different techniques to enhance biogas production. In order to achieve the aforementioned state, it is essential to ensure increased activity of microorganisms that contribute to the anaerobic fermentation. The metabolic activity of microorganisms is preconditioned by availability of easily decomposable solids. Adding of bacterial and enzymatic cultures into a fermented substrate represents one of the possibilities. The enzymes contained in this preparation are responsible for better exposing methanogenic bacteria to the material. The tested bio-enzymatic preparation, APD BIO GAS, is a mixture that contains bacteria and enzymes which are essential for the efficient progress of anaerobic fermentation. The reference biogas laboratory of the Mendel University in Brno was used for the purpose of testing of APD BIOGAS in mesophilic conditions of anaerobic fermentation on a substrate consisting of a mixture of maize silage and liquid manure. The producer of this preparation declare enhancement of quality and quantity of developed biogas, elimination of smell level of the rest of fermentation its higher homogenity. For the test were used lab scale fermenters of batch type with work volume 0.12 m3. An increase of biogas production by 15% was determined in connection with addition of the preparation, also with higher decrease of total solids and decrease of organic substance in total solids in the fermenter where were used this preparation