36 research outputs found
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization
Mammalian sperm acrosome contains several glycohydrolases thought to aid in the dispersion and digestion of vestments surrounding the egg. In this study, we have used multiple approaches to examine the origin of acrosome-associated glycohdyrdolases. Mixed spermatogenic cells, prepared from rat testis, were separated by unit gravity sedimentation. The purified germ cells (spermatocytes [SP], round spermatids [RS], and elongated/condensed spermatids [E/CS]) contained several glycohydrolase activities. Metabolic labeling in the cell culture, immunoprecipitation, and autoradiographic approaches revealed that β-D-galactosidase was synthesized in SP and RS in 88/90 kDa forms which undergo processing in a cell-specific manner. Immunohistochemical approaches demonstrated that the enzyme was localized in Golgi membranes/vesicles, and lysosome-like structures in SP and RS, and forming/formed acrosome of E/CS
Analytical and empirical evaluation of the impact of Gaussian noise on the modulations employed by Bluetooth Enhanced Data Rates
Rapid screening for Chlamydia trachomatis infection by detecting α-mannosidase activity in urogenital tract specimens
Rat epididymal luminal fluid acid β-d-galactosidase optimally hydrolyses glycoprotein substrate at neutral pH
Asparagine-linked glycoprotein biosynthesis in rat epididymis. Presence of a mannosidase II-like enzyme
A ram epididymal secretory protein shares common antigenic determinants with rat epididymal proteins and human seminal plasma proteins
International audienc
A ram epididymal secretory protein shares common antigenic determinants with rat epididymal proteins and human seminal plasma proteins
International audienc