Effect of water acidification on some morphological, digestive and production traits in broiler chickens

The objective of the study was to determine the effect of acidifier added to drinking water on growth rate, performance index, flock uniformity, weight of edible giblets and immune organs, fermentation process in the caecum and excretion of dry matter and crude protein in broiler chicks. One hundred one day old broiler chicks were assigned in two equal groups. Birds of the test group were supplied with drinking water with the addition of acidifying preparation (in amount of 2 mL/L during the whole experiment).Acidification of drinking water had positive effect on growth rate during finisher phase (P<0.05) and reduction of crude protein in faeces (P<0.001). Although not statistically signifi-cant, water acidification increased flock body weight uniformity. No significant effect of water acidi-fication were observed on performance index, weight of edible giblets and immune organs, pH and concentration of short chain fatty acids in caecum content as well as content of dry matter in faeces

Rizikové faktory potravového reťazca V

Abstract In this study the effect of single cadmium, cadmium + selenium and cadmium + zinc administration on the ovarian structure of Japanese quails was studied. The morphometric analysis of the relative volume of primary follicles detected the highest value in control group with a similar value in the group with administration of cadmium with selenium. Lower relative volume is reported in group with cadmium and zinc administration and the group with simple cadmium administration (P&lt;0.05). The relative volume of growing follicles was very similar in all studied groups (11.33 -15.35%), and the relative volume of stroma was very stable (82.59 -86.45%). In the evaluation of the number of follicles undergoing atresia significantly higher number of atretic primary follicles as well as growing follicles in the group with cadmium administration and cadmium with selenium administration in comparison with control group was found. In comparison of normal and atretic follicles we report the most negative effect on ovarian structure in group with cadmium administration. Selenium co-administration show protective effects but only the co-administration with zinc prevent significant cadmium ovarian alterations

Quadruple-peaked spectral line profiles as a tool to constrain gravitational potential of shell galaxies

Stellar shells observed in many giant elliptical and lenticular as well as a few spiral and dwarf galaxies, presumably result from galaxy mergers. Line-of-sight velocity distributions of the shells could, in principle, if measured with a sufficiently high S/N, constitute one of methods to constrain the gravitational potential of the host galaxy. Merrifield & Kuijken (1998) predicted a double-peaked line profile for stationary shells resulting from a nearly radial minor merger. In this paper, we aim at extending their analysis to a more realistic case of expanding shells, inherent to the merging process, whereas we assume the same type of merger and the same orbital geometry. We use analytical approach as well as test particle simulations to predict the line-of-sight velocity profile across the shell structure. Simulated line profiles are convolved with spectral PSFs to estimate the peak detectability. The resulting line-of-sight velocity distributions are more complex than previously predicted due to non-zero phase velocity of the shells. In principle, each of the Merrifield & Kuijken (1998) peaks splits into two, giving a quadruple-peaked line profile, which allows more precise determination of the potential of the host galaxy and, moreover, contains additional information. We find simple analytical expressions that connect the positions of the four peaks of the line profile and the mass distribution of the galaxy, namely the circular velocity at the given shell radius and the propagation velocity of the shell. The analytical expressions were applied to a test-particle simulation of a radial minor merger and the potential of the simulated host galaxy was successfully recovered. The shell kinematics can thus become an independent tool to determine the content and distribution of the dark matter in shell galaxies, up to ~100 kpc from the center of the host galaxy.Comment: 15 pages, 16 figures | v2: accepted for publication in A&A, minor language correction

Political stringency, infection rates, and higher education students' adherence to government measures in the Nordic countries and the UK during the first wave of the COVID-19 outbreak

Understanding predictors of adherence to governmental measures to prevent the spread of the COVID-19 is fundamental to guide health communication. This study examined whether political stringency and infection rates during the first wave of the pandemic were associated with higher education students' adherence to COVID-19 government measures in the Nordic countries (Denmark, Finland, Norway, Iceland, and Sweden) and the United Kingdom. Both individual- and country-level data were used in present study. An international cross-sectionalsubsample (n = 10,345) of higher-education students was conducted in May–June 2020 to collect individual-level information on socio-demographics, study information, living arrangements, health behaviors, stress, and COVID-19-related concerns, including adherence to government measures. Country-level data on political stringency from the Oxford COVID-19 Government Response Tracker and national infection rates were added to individual-level data. Multiple linear regression analyses stratified by country were conducted. Around 66% of students reported adhering to government measures, with the highest adherence in the UK (73%) followed by Iceland (72%), Denmark (69%), Norway (67%), Finland (64%) and Sweden (49%). Main predictors for higher adherence were older age, being femaleand being worried about getting infected with COVID-19 (individual-level), an increase in number of days since lockdown, political stringency, and information about COVID-19 mortality rates (country-level). However, incidence rate was an inconsistent predictor, which may be explained by imperfect data quality during the onset of the pandemic. We conclude that shorter lockdown periods and political stringency are associated with adherence to government measures among higher education students at the outset of the COVID-19 pandemic.Peer reviewe

Satellite DNA in Paphiopedilum subgenus Parvisepalum as revealed by high-throughput sequencing and fluorescent in situ hybridization

Background: Satellite DNA is a rapidly diverging, largely repetitive DNA component of many eukaryotic genomes. Here we analyse the evolutionary dynamics of a satellite DNA repeat in the genomes of a group of Asian subtropical lady slipper orchids (Paphiopedilum subgenus Parvisepalum and representative species in the other subgenera/sections across the genus). A new satellite repeat in Paphiopedilum subgenus Parvisepalum, SatA, was identified and characterized using the RepeatExplorer pipeline in HiSeq Illumina reads from P. armeniacum (2n = 26). Reconstructed monomers were used to design a satellite-specific fluorescent in situ hybridization (FISH) probe. The data were also analysed within a phylogenetic framework built using the internal transcribed spacer (ITS) sequences of 45S nuclear ribosomal DNA. Results: SatA comprises c. 14.5% of the P. armeniacum genome and is specific to subgenus Parvisepalum. It is composed of four primary monomers that range from 230 to 359 bp and contains multiple inverted repeat regions with hairpin loop motifs. A new karyotype of P. vietnamense (2n = 28) is presented and shows that the chromosome number in subgenus Parvisepalum is not conserved at 2n = 26, as previously reported. The physical locations of SatA sequences were visualised on the chromosomes of all seven Paphiopedilum species of subgenus Parvisepalum (2n = 26–28), together with the 5S and 45S rDNA loci using FISH. The SatA repeats were predominantly localisedin the centromeric, peri-centromeric and sub-telocentric chromosome regions, but the exact distribution pattern was species-specific. Conclusions: We conclude that the newly discovered, highly abundant and rapidly evolving satellite sequence SatA is specific to Paphiopedilum subgenus Parvisepalum. SatA and rDNA chromosomal distributions are characteristic of species, and comparisons between species reveal that the distribution patterns generate a strong phylogenetic signal. We also conclude that the ancestral chromosome number of subgenus Parvisepalum and indeed of all Paphiopedilum could be either 2n = 26 or 28, if P. vietnamense is sister to all species in the subgenus as suggested by the ITS data