15 research outputs found
The effect of cultivation media and washing whole-cell biocatalysts on monoamine oxidase catalyzed oxidative desymmetrization of 3-azabicyclo[3,3,0]octane
Fully Printed Flexible Humidity Sensor
AbstractA humidity sensor that employs interdigitated capacitors (IDC) printed with silver (Ag) nanoparticle based ink on a flexible poly ethylene terephthalate (PET) substrate was successfully fabricated using gravure printing process. Thicknesses of 1ÎŒm and 500ÎŒm of a humidity sensitive polymer poly (2-hydroxyethyl methacrylate) (pHEMA) was deposited on the IDCs by means of gravure printing. The capacitive response of the sensor towards relative humidity (RH) was measured in the range of 30% RH to 80% RH; the maximum percentage change in capacitance was 172% at 80% RH when compared to base capacitance at 30% RH. The humidity response of the printed sensor revealed a very high stability with a maximum error of 0.6% and 0.8% from the average value at 40% RH and 60% RH, respectively
Chemo-biocatalytic one-pot two-step conversion of cyclic amine to lactam using whole cell monoamine oxidase
BACKGROUND:
Most biocatalysts currently involved in oneâpot chemoenzymatic cascades are pure enzymes, while whole cells and crude enzyme extracts remain unexplored. This work aims to develop a chemoâbiocatalytic oneâpot twoâstep system involving whole cell monoamine oxidase (MAO, EC 1.4.3.4) coupled with a Cuâbased oxidative system (CuI/H2O2) for the transformation of 1,2,3,4âtetrahydroisoquinoline (THIQ) to 3,4âdihydroisoquinolinâ1(2H)âone (DHIO).
RESULTS:
MAOâN variants D9 and D11 were tested as whole cell and crude lysate biocatalysts for biological oxidation. Whole Escherichia coli OverExpress C43(DE3) cells expressing MAOâN D9 showed the best performance (Vmax = 36.58 mmol Lâ1 hâ1, KM = 8.124 mmol Lâ1, maximum specific productivity 89.3 ÎŒmol minâ1 gâ1DCW) and were employed in combination with CuI/H2O2 in a sequential oneâpot twoâstep process. The biotransformation was scaledâup to the initial volume of 25 mL and after triple THIQ feeding, 48.2 mmol Lâ1 of the intermediate 3,4âdihydroisoquinoline (DHIQ) was obtained with a yield of 71.3%. Afterwards, chemical catalysts (1 mol% CuI and 10 eq. H2O2) were added to the biologically produced DHIQ, which was transformed to âŒ30 mmol Lâ1 DHIO at 69.4% overall yield.
CONCLUSION:
As MAOâN variants have wide substrate specificity, this work broadens the portfolio of oneâpot chemoenzymatic processes employing whole cell biocatalysts, representing an alternative to using pure enzymes
Surface Treatments for Inkjet Printing onto a PTFE-Based Substrate for High Frequency Applications
This document is the Accepted Manuscript version of a Published Work that appeared in final form in
the journal Industrial and Engineering Chemistry Research [copyright © American Chemical Society] after peer review and technical editing by the publisher.
To access the final edited and published work see: http://pubs.acs.org/doi/abs/10.1021/ie4006639Inkjet printing onto laminates for use in high frequency applications (high frequency laminates)
is challenging, due to the substrate surface roughness present after etching away the copper
layer(s). This has a detrimental effect on interconnect losses as the frequency increases. In this
paper, different surface treatments to reduce the surface roughness of a typical high frequency
laminate (RO3006) are investigated. In particular, the importance of matching the substrate
surface energy to the ink to achieve a smooth coated layer for the case of a UV cured insulator is
demonstrated. This is achievable within the parameters of heating the platen, which is a more
flexible approach compared to modifying the ink to improve the ink-substrate interaction. In
printing onto the surface modified substrates, the substrate roughness was observed to affect the
printed line width significantly. A surface roughness factor was introduced to take into account
the phenomenon by modifying the original formula of Smith et al. Lastly, the authors show that
the printed line widths are also influenced by the surface tension arising from charges present on
the surface modified substrates