The synergistic action of imidacloprid and flumethrin and their release kinetics from collars applied for ectoparasite control in dogs and cats

<p>Abstract</p> <p>Background</p> <p>The control of tick and flea burdens in dogs and cats has become essential to the control of important and emerging vector borne diseases, some of which are zoonoses. Flea worry and flea bite hypersensitivity are additionally a significant disease entity in dogs and cats. Owner compliance in maintaining the pressure of control measures has been shown to be poor. For these reasons efforts are continuously being made to develop ectoparasiticides and application methods that are safe, effective and easy to apply for pet owners. A new polymer matrix collar has recently been developed which is registered for 8 months use in cats and dogs. The basic properties of this collar have been investigated in several <it>in vitro </it>and <it>in vivo </it>studies.</p> <p>Methods</p> <p>The effects of imidacloprid, flumethrin and the combination were evaluated in vitro by means of whole cell voltage clamp measurement experiments conducted on isolated neuron cells from <it>Spodoptera frugiperda</it>. The in vitro efficacy of the two compounds and the combination against three species of ticks and their life stages and fleas were evaluated in a dry surface glass vial assay. The kinetics of the compounds over time in the collar were evaluated by the change in mass of the collar and measurement of the surface concentrations and concentrations of the actives in the collar matrix by HPLC. Hair clipped from collar treated dogs and cats, collected at various time points, was used to assess the acaricidal efficacy of the actives ex vivo.</p> <p>Results</p> <p>An <it>in vitro </it>isolated insect nerve model demonstrated the synergistic neurotoxic effects of the pyrethroid flumethrin and the neonicotinoid imidacloprid. An <it>in vitro </it>glass vial efficacy and mortality study against various life stages of the ticks <it>Ixodes ricinus, Rhipicephalus sanguineus </it>and <it>Dermacentor reticulatus </it>and against the flea (<it>Ctenocephalides felis</it>) demonstrated that the combination of these products was highly effective against these parasites. The release kinetics of these actives from a neck collar (compounded with 10% imidacloprid and 4.5% flumethrin) was extensively studied in dogs and cats under laboratory and field conditions. Acaricidal concentrations of the actives were found to be consistently released from the collar matrix for 8 months. None of the collar studies in dogs or cats were associated with any significant collar related adverse event.</p> <p>Conclusion</p> <p>Here we demonstrated the synergism between the pyrethroid flumethrin and the neonicotinoid imidacloprid, both provided in therapeutically relevant doses by a slow release collar matrix system over 8 months. This collar is therefore a convenient and safe tool for a long-term protection against ectoparasites.</p

Considerations in the evaluation of concrete structures for continued service in aged Nuclear Power Plants (NPPs)

Currently, there are /approximately/119 commercial nuclear power plants (NPPs) in the US either under construction, operating at low-to-full power, or awaiting an operating license. Together, these units have a net generating capacity of /approximately/110 GW(e). Assuming no life extension of present facilities, the operating licenses for these plants will start to expire in the middle of the next decade with Yankee Rowe being the first plant to attain this status. Where it is noted that with no life extension of facilities, a potential loss of electrical generating capacity in excess of 75 GW(e) could occur during the time period 2006 to 2020 when the operating licenses of 80 to 90 NPPs are scheduled to expire. A potential timely and cost-effective solution to meeting future electricity demand, which has worked well for non-nuclear generating plants, is to extend the service life (operating licenses) of existing NPPs. Since the concrete components in these plants provide a vital safety function, any continued service considerations must include an in-depth assessment of the safety-related concrete structures. 7 refs

Autonomous Airborne Wind Energy Systems: Accomplishments and Challenges

Airborne wind energy (AWE) is a fascinating technology to convert wind power into electricity with an autonomous tethered aircraft. Deemed a potentially game-changing solution, AWE is attracting the attention of policy makers and stakeholders with the promise of producing large amounts of cost-competitive electricity with wide applicability worldwide. Since the pioneering experimental endeavors in the years 2000-2010, there has been a clear technology convergence trend and steady progress in the field. Today, AWE systems can operate automatically with minimal supervision in all operational phases. A first product is also being commercialized. However, all-around fully autonomous operation still presents important fundamental challenges that are conceptually similar to those of other systems that promise to change our lives, such as fully autonomous passenger cars or service drones. At the same time, autonomous operation is necessary to enable large-scale AWE, thus combining challenging fundamental problems with high potential impact on society and the economy. This article describes the state of the art of this technology from a system perspective and with a critical view on some fundamental aspects, presents the latest automatic control results by prominent industrial players, and finally points out the most important challenges on the road to fully autonomous AWE systems