Effects of family control on the degree and type of diversification: empirical evidence for business groups

This article analyzes the impact of ownership structure on corporate diversification,with reference to large listed family business groups. By considering agency theoryand socioemotional wealth, the study examines the relationship between family own-ership, concentration of ownership, and degree and type of diversification. The studyconsiders 99 Spanish listed business groups (50 family-controlled- and 49 nonfamily-controlled groups) and considers diversification of business group as the focus ofanalysis. The results show how family business groups present a lower preference forunrelated diversification than related diversification. There is also a nonlinear rela-tionship between the concentration of ownership in family groups and the degree ofdiversification, showing different behaviors in family groups according to sharesowned by the family's leading shareholders. This article contributes to the literatureby providing a more precise identification of the corporate strategy adopted by busi-ness groups and establishing new evidence about the impact of family control ondiversification strategies and the differences regarding nonfamily business groups

HTLV-1 Tax Mediated Downregulation of miRNAs Associated with Chromatin Remodeling Factors in T Cells with Stably Integrated Viral Promoter

RNA interference (RNAi) is a natural cellular mechanism to silence gene expression and is predominantly mediated by microRNAs (miRNAs) that target messenger RNA. Viruses can manipulate the cellular processes necessary for their replication by targeting the host RNAi machinery. This study explores the effect of human T-cell leukemia virus type 1 (HTLV-1) transactivating protein Tax on the RNAi pathway in the context of a chromosomally integrated viral long terminal repeat (LTR) using a CD4+ T-cell line, Jurkat. Transcription factor profiling of the HTLV-1 LTR stably integrated T-cell clone transfected with Tax demonstrates increased activation of substrates and factors associated with chromatin remodeling complexes. Using a miRNA microarray and bioinformatics experimental approach, Tax was also shown to downregulate the expression of miRNAs associated with the translational regulation of factors required for chromatin remodeling. These observations were validated with selected miRNAs and an HTLV-1 infected T cells line, MT-2. miR-149 and miR-873 were found to be capable of directly targeting p300 and p/CAF, chromatin remodeling factors known to play critical role in HTLV-1 pathogenesis. Overall, these results are first in line establishing HTLV-1/Tax-miRNA-chromatin concept and open new avenues toward understanding retroviral latency and/or replication in a given cell type

A new species of Pandaridae (Copepoda), from the whale shark Rhincodon typus (Smith)

The copepod Pandarus rhincodonicus sp. nov. (Copepoda: Pandaridae), collected from whale sharks, Rhincodon typus (Smith, 1828) (Orectolobiformes: Rhincodontidae), swimming off the North West Cape of Western Australia, is described. Pandarus rhincodonicus is closely related to Pandarus cranchii Leach, 1819. However, P. rhincodonicus differs from P. cranchii in: the armature of the posterior margin of the cephalon; the plates of segment 2 extend almost to the limit of segment 4; the shape of the caudal rami and shape of the dorsal abdominal plate; and the distribution and numbers of spines and setae on thoracic appendages. It is suggested that P. rhincodonicus is a commensal

Experimental Characterization of Hb Flurlingen (HBA2: c.177 C > G, p.His > Gln) and Hb Boghé (HBA2: c.177 C > A, p.His > Gln) Reveals Contradictory HBA2 Expression and Translation Patterns Despite Identical Amino Acid Substitutions

In this study, we describe the clinical features and provide experimental analyses of Hb Flurlingen (HBA2: c.177 C > G, p.His > Gln) that contrasted with Hb Boghé (HBA2: c.177 C > A, p.His > Gln). Despite the identical amino acid substitution in both variants, Hb Flurlingen shows the phenotype of α-thalassemia (α-thal), whereas Hb Boghé has no impact on α2-globin (HBA2) production. For in vitro transcription analysis, HBA2 expression constructs carrying the HBA2-WT (wild type), Hb Flurlingen and Hb Boghé sequences were generated and expressed in human bladder carcinoma 5637 cells for downstream analyses by quantitative real time-polymerase chain reaction (qReTi-PCR) and immunofluorochemistry (IFC). In silico analysis of secondary folding structures of the HBA2-WT, Hb Flurlingen and Hb Boghé mRNA sequences was performed using Mfold software. The gene transcription and translation analyses revealed that cells transfected with the Hb Flurlingen construct had significantly lower HBA2 transcription (−55.4%, p ≤ 0.01) and reduced protein synthesis when compared to the wild type group. In contrast, cells transfected with the Hb Boghé construct showed no significant changes in HBA2 transcription or translation activities when compared to the wild type group. The in silico prediction of possible effects of these mutations on the folding structures of the HBA2 transcripts showed a change of secondary folding pattern in the Hb Flurlingen transcript when compared to those of HBA2-WT and Hb Boghé. Our experimental findings support the clinical presentation of an α-thalassemic phenotype for Hb Flurlingen in contrast with Hb Boghé, despite identical amino acid substitutions. The results confirm the importance of experimental analysis in establishing the impact of novel base substitutions