57 research outputs found
Machine-Related Backgrounds in the SiD Detector at ILC
With a multi-stage collimation system and magnetic iron spoilers in the
tunnel, the background particle fluxes on the ILC detector can be substantially
reduced. At the same time, beam-halo interactions with collimators and
protective masks in the beam delivery system create fluxes of muons and other
secondary particles which can still exceed the tolerable levels for some of the
ILC sub-detectors. Results of modeling of such backgrounds in comparison to
those from the e+ e- interactions are presented in this paper for the SiD
detector.Comment: 29 pages, 34 figures, 7 table
Atenção nos cuidados de enfermagem das escaras quanto às associações terapêuticas
Microfluidic chip for fast bioassays—evaluation of binding parameters
A seven channel polystyrene (PS) microchip has been constructed using a micromilling machine and a high-temperature assembling. Protein A (PA) has been immobilized by a passive sorption on the microchannel walls. Two bioaffinity assays with human immunoglobulin G (hIgG) as a ligand have been carried out. (i) PA as the receptor and fluorescently labeled hIgG (FITC-hIgG) as the ligand, (ii) PA as the receptor with hIgG as the quantified ligand and fluorescently labeled goat anti-human IgG (FITC-gIgG) as the secondary ligand. One incubation step of the assays took only 5 min instead of hours typical for enzyme-linked immunosorbent assay applications. Calibration curves of the dependence of a fluorescence signal on the hIgG concentration in a sample have been obtained in one step due to a parallel arrangement of microchannels. A mathematical model of the PA-FITC-hIgG complex formation in the chip has been developed. The values of the kinetic constant of the PA-FITC-hIgG binding (kon=5.5 m3 mol−1 s−1) and the equilibrium dissociation constant of the formed complex (Kd≤3×10−6 mol m−3) have been obtained by fitting to experimental data. The proposed microchip enables fast evaluation of kinetic and equilibrium constants of ligand-receptor bioaffinity pairs and the ligand quantification. As the use of microfluidic chips for immunoassays is often limited by price, we used procedures and chemicals that allow for an inexpensive construction and operation of the microdevice, e.g., temperature assembling as a fabrication technique, detection via an ordinary digital camera, nonspecific polystyrene as a substrate, passive sorption of biomolecules as an immobilization technique, etc
Quantum-chemical simulation of interaction of polycaproamide with a lithium chloride solution in dimethylacetamide
Modified Stennert’s Protocol in Treating Acute Peripheral Facial Nerve Paralysis: Our Experience
Effects of Stimulation of the Substantia Innominata and Caudate Nucleus on Postsynaptic Reactions of Neurons of the Cat Somatosensory Cortex Activated by Excitation of Nociceptors
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