Prevalence of mixed genotype hepatitis C virus infections in the UK as determined by genotype‐specific PCR and deep sequencing

The incidence of mixed genotype hepatitis C virus infections in the UK is largely unknown. As the efficacy of direct acting antivirals is variable across different genotypes, treatment regimens are tailored to the infecting genotype, which may pose issues for the treatment of underlying genotypes within undiagnosed mixed genotype HCV infections. There is therefore a need to accurately diagnose mixed genotype infections prior to treatment. PCR-based diagnostic tools were developed to screen for the occurrence of mixed genotype infections caused by the most common UK genotypes, 1a and 3, in a cohort of 506 individuals diagnosed with either of these genotypes. The overall prevalence rate of mixed infection was 3.8% however this rate was unevenly distributed, with 6.7% of individuals diagnosed with genotype 3 harbouring genotype 1a strains and only 0.8% of samples from genotype 1a patients harbouring genotype 3 (p<0.05). Mixed infection samples consisted of a major and a minor genotype, with the latter constituting less than 21% of the total viral load and, in 67% of cases, less than 1% of the viral load. Analysis of a subset of the cohort by Illumina PCR-next generation sequencing resulted in a much greater incidence rate than obtained by PCR. This may have occurred due to the non-quantitative nature of the technique and despite the designation of false positive thresholds based on negative controls

Global Care of Orphaned and Vulnerable Children

Care for orphaned and vulnerable children living in poverty is a widespread concern affecting millions worldwide. This presentation will focus on the current literature surrounding physical, emotional and environmental care in the following countries: Mozambique, the USA and the Philippines. There is a correlation among these three countries involving the lack of food, health care and education. There will be an emphasis on the national policies implemented within each country that seek to ensure sustainability and quality in care. This presentation will also focus on the positive change that is being fulfilled by both government and non-government organizations passionate about the issue in each specific country. Some of these agencies include Naomi’s Heart Mission, The Creche and Local Organizations in Ohio

On the relation between adjacent inviscid cell type solutions to the rotating-disk equations

Over a large range of the axial coordinate a typical higher-branch solution of the rotating-disk equations consists of a chain of inviscid cells separated from each other by viscous interlayers. In this paper the leading-order relation between two adjacent cells will be established by matched asymptotic expansions for general values of the parameter appearing in the equations. It is found that the relation between the solutions in the two cells crucially depends on the behaviour of the tangential velocity in the viscous interlayer. The results of the theory are compared with accurate numerical solutions and good agreement is obtained

Post-mortem findings and causes of death of harbour porpoises (Phocoena phocoena) stranded from 1990 to 2000 along the coastlines of Belgium and northern France

peer reviewedBetween the,ears 1990 and 2000, an attempt was made to determine the causes of death of 55 harbour porpoises stranded along the Belgian and northern French coasts. From 1990 to 1996, only five carcasses were collected as against seven in 1997, eight in 1998, 27 in 1999 and eight in 2000. The sex ratio was normal and most of the animals were juvenile. The most common findings were emaciation, severe parasitosis and pneumonia. A few cases of fishing net entanglement were observed. The main microscopical lesions were acute pneumonia, massive lung oedema, enteritis, hepatitis and gastritis. Encephalitis was observed in six cases. No evidence of morbillivirus infection was detected. Pneumonia was associated with bacteria or parasites, or both. The causes of death and the lesions were similar to those previously reported in other countries bordering the North Sea. The cause of the increased numbers of carcasses in 1999 was unclear but did not include viral epizootics or net entanglement. A temporary increase in the porpoise populatiou in the southern North Sea may have been responsible. (C) 2002 Elsevier Science Ltd. All rights reserved

Identification of F-box only protein 7 as a negative regulator of NF-kappaB signalling.

The nuclear factor κB (NF-κB) signalling pathway controls important cellular events such as cell proliferation, differentiation, apoptosis and immune responses. Pathway activation occurs rapidly upon TNFα stimulation and is highly dependent on ubiquitination events. Using cytoplasmic to nuclear translocation of the NF-κB transcription factor family member p65 as a read-out, we screened a synthetic siRNA library targeting enzymes involved in ubiquitin conjugation and de-conjugation for modifiers of regulatory ubiquitination events in NF-κB signalling. We identified F-box protein only 7 (FBXO7), a component of Skp, Cullin, F-box (SCF)-ubiquitin ligase complexes, as a negative regulator of NF-κB signalling. F-box protein only 7 binds to, and mediates ubiquitin conjugation to cIAP1 and TRAF2, resulting in decreased RIP1 ubiquitination and lowered NF-κB signalling activity

An asymptotic treatment of the Elenbaas–Heller equation for a radiating wall‐stabilized high‐pressure gas‐discharge arc

An asymptotic analysis of the Elenbaas–Heller equation for a radiating wall-stabilized high-pressure gas-discharge arc is given. This analysis applies when the operating temperatures within the arc are lower than the ionization temperature by an order of magnitude. It is shown that for arcs that are radiating highly efficiently a further asymptotic treatment can be given. It is shown under what conditions, governed by a dimensionless parameter M, this limiting case prevails. Comparison with earlier results put forward by Zollweg [J. Appl. Phys. 49, 1077 (1978)] shows satisfactory agreement