Using various antigen preparations to produce monoclonal antibodies against bovine leukaemia virus (BLV) gp51SU

The objective of this study was to compare different antigen preparations to produce monoclonal antibodies against bovine leukaemia virus gp51SU. The four antigen preparations for immunization of BALB/c mice were: CL: BLV-FLK cell lysate, UF: a fraction of CL (between 30 and 100 kDa), WVP: whole virus particles and SP: with ion exchange chromatography, gp51SU was semipurified. A total of nine successful fusions were performed which resulted in production of 23 monoclonal antibodies (mAbs) specific against gp51SU. The highest ratio of specific hybridoma colonies in each fusion was with SP preparation. Based on the reactivity of the mAbs in Western blotting, mAbs were classified into four groups: anti-gp51SU (23 mAbs), anti-gp30TM (8 mAbs), anti-Pr72 (5 mAbs) and antibodies against other viral proteins (7 mAbs). Some of the anti-gp51SU mAbs reacted with more than one band in Western blotting, suggesting that these colonies recognized not only gp51SU but also its precursors.Jafari Jozani, R.; Taheri, M.; Khazraiinia, P. and Hemmatzadeh, F

Preparation and Characterization of Co-Mo Catalyst Supported on CNT Coated Cordierite Monoliths Utilized for Naphta HDS Process

AbstractIn the present study, an acid treatment of the cordierite monolith followed by coating of the CNT onto it by means of catalytic chemical-vapor deposition (CCVD) of methane over CoMo catalyst was performed. The resulting material utilized as the supports for the HDS catalyst onto which was deposited Co and Mo species. The as-prepared samples were characterized by the BET, XRD, FESEM and ICP-MS techniques. BET experiments showed that a significant increase in specific surface area and pore volume was observed, and a suitable pore size distribution was obtained after acid pretreatment. The FESEM images of CNT coated monolith showed that CNTs were distributed uniformly on the surface of monolith substrate, which led to a high BET surface area and relatively good adhered mesoporous layer of CNTs. Ultimately, the catalytic activity of the resulting catalysts was studied in the HDS reaction of Naphta. It was concluded that the activity of CoMo/FACNT catalyst was higher than that of a supported catalyst. Furthermore, the reaction over the this material decreased the sulfur content of Naphtha from 2670 to 53ppm

Production and characterization of monoclonal antibody to bovine leukemia virus gp51-SU

Enzootic bovine leukemia (EBL) is a disease of cattle with worldwide distribution. The bovine leukemia virus (BLV), is a typical type C retrovirus, has four genes: gag, pol, pro and env, flanked by long terminal repeats (LTR) and lacking oncogenes. Gene env encodes a precursor protein named Pr72 env which undergoes glycosylation and lysis, giving rise to an envelope glycoprotein gp51-SU with a total of 12 epitopes. The objective of the present study was to produce a panel of monoclonal antibodies (mAbs) against BLV-gp51-SU following the standard immunization protocols. The antigen preparations included cellular lysates, a fraction of cellular lysates to study antibodies against BLV proteins precursors, whole virus particles and semipurified gp51 -SU. Both route of injection (SC and IP) resulted in hyperimmunized mice. As indicated by indirect-ELISA and also yielded high fusion efficiency. The hybridoma colonies were stable and had a tendency to save their ability to secrete antibodies. Fusions produced five positive colonies in screening tests, two of which were against gp51-SU. The targeted hydrophobic domain of the gp51-SU molecule was detected by one of the colonies of mAbs. The two interested mAbs were used for immunohistochemistry on lymph node sections of a cow with lymphosarcoma which can detect immunoreactive materials in sections and also in H&E staining. We conclude the produced panel of mAbs is able to be used in detection of gp51-SU and a new epitope identified on this molecule could be used in IHC method for diagnosis of BLV infection. © IDOSI Publications, 2011.Raziallah Jafari Joozani, Parvaneh Khazrai Nia, Mohammad Taheri, Farhid Hemmatzadeh and Javad Ashrafihela

Hydrodesulphurization Nanocatalyst, Its Use and a Process for Its Production

A nano-supported hydrodesulphurization (HDS) catalyst is prepared for hydrodesulphurization of hydrocarbonaceous feed stock. The catalyst can be prepared through different methods and also used under milder conditions than those required for conventionally used HDS catalysts, but can also function under other hydrodesulphurization operating condition

Prediction of k-records from a general class of distributions under balanced type loss functions

Absolute value error loss, Balanced loss function, Bayes prediction, Conditional median prediction, Maximum likelihood prediction, LINEX loss, Record values,

Minimax estimation of constrained parametric functions for discrete families of distributions

Minimax estimation, Restricted parameter space, Discrete distributions, Squared error loss, Zero count probability, Odds-ratio, Binomial variance, Negative Binomial variance,