Bravo/Nr-CAM Is Closely Related to the Cell Adhesion Molecules L1 and Ng-CAM and Has a Similar Heterodimer Structure

Diverse cell-surface molecules of the nervous system play an important role in specifying cell interactions during development. Using a method designed to generate mAbs against neural surface molecules of defined molecular weight, we have previously reported on the surface protein, Bravo, found in the developing avian retinotectal system. Bravo is immunologically detected on developing optic fibers in the retina, but absent from distal regions of the same fibers in the tectum. We have isolated cDNA clones encompassing the entire coding region of Bravo, including clones containing five alternative sequences of cDNA. These putative alternatively spliced sequences encode stretches of polypeptide ranging in length from 10-93 amino acids and are predicted to be both extra- and intracellular. The deduced primary structure of Bravo reveals that, like the cell adhesion molecules (CAMs) chicken Ng- CAM and mouse L1, Bravo is composed of six Ig-like domains, five fibronectin type III repeats, a transmembrane domain, and a short cytoplasmic region. Recently, the cDNA sequence of a related molecule, Nr-CAM, was reported and its possible identity with Bravo discussed (Grumet, M., V. Mauro, M. P. Burgoon, G. E. Edelman, and B. A. Cunningham. 1991. J. Cell Biol. 113:1399-1412). Here we confirm this identity and moreover show that Bravo is found on Muller glial processes and end-feet in the developing retina. In contrast to the single polypeptide chain structure of Nr-CAM reported previously, we show that Bravo has a heterodimer structure composed of an alpha chain of M(r) 140/130 and a beta chain of 60-80 kD. As with L1 and Ng-CAM, the two chains of Bravo are generated from an intact polypeptide by cleavage at identical locations and conserved sites within all three molecules (Ser-Arg/Lys-Arg). The similar domain composition and heterodimer structure, as well as the 40% amino acid sequence identity of these molecules, defines them as an evolutionarily related subgroup of CAMs. The relationship of Bravo to molecules known to be involved in cell adhesion and process outgrowth, combined with its pattern of expression and numerous potential isoforms, suggests a complex role for this molecule in cell interactions during neural development

Broadband X-ray spectrum of the newly discovered broad line radio galaxy IGR J21247+5058

In this paper we present radio and high energy observations of the INTEGRAL source IGR J21247+5058, a broad line emitting galaxy obscured by the Galactic plane. Archival VLA radio data indicate that IGR J21247+5058 can be classified as an FRII Broad Line Radio Galaxy. The spectrum between 610 MHz and 15 GHz is typical of synchrotron self-absorbed radiation with a peak at 8 GHz and a low energy turnover; the core fraction is 0.1 suggestive of a moderate Doppler boosting of the base of the jet. The high energy broad-band spectrum was obtained by combining XMM-Newton and Swift/XRT observation with INTEGRAL/IBIS data. The 0.4-100 keV spectrum is well described by a power law, with slope $\Gamma$=1.5, characterised by complex absorption due to two layers of material partially covering the source and a high energy cut-off around 70-80 keV. Features such as a narrow iron line and a Compton reflection component, if present, are weak, suggesting that reprocessing of the power law photons in the accretion disk plays a negligible role in the source.Comment: 7 pages, 7 figures, 3 tables, accepted for pubblication on MNRA

The teaching and assessment of computer aided design -a competence based or theory based approach?

The role of CAD within an overall Product Design teaching framework, at Higher Education level, is explored and reviewed. This is considered from three perspectives: Student- their expectations in terms of theoretical knowledge and competence. Academic- theory based requirements for successful achievement of learning outcomes. Employer- vocational competence requirements for Product Design graduates. With emphasis on the experiences of undergraduate and graduate Product Design students at the University of Derby, and prospective or actual graduate employers, the unique expectations and requirements within each category are considered. The overall means of resolving these, often conflicting, requirements into the CAD teaching framework of a Product Design degree, operating within a modular scheme, are then explored. The implications of these demands on teaching and assessment strategies with the subsequent resource issues are also reviewed. A teaching strategy with a well linked combination of both theory and competence with, where possible, cross-modular innovative assessment strategies is considered to be an effective approach

Topologically Restricted Appearance in the Developing Chick Retinotectal System of Bravo, a Neural Surface Protein: Experimental Modulation by Environmental Cues

A novel neural surface protein, Bravo, shows a pattern of topological restriction in the embryonic chick retinotectal system. Bravo is present on the developing optic fibers in the retina; however, retinal axons in the tectum do not display Bravo. The appearance of Bravo in vitro is modulated by environmental cues. Axons growing out from retinal explants on retinal basal lamina, their natural substrate, express Bravo, whereas such axons growing on collagen do not. Retinal explants provide a valuable system to characterize the mechanism of Bravo restriction, as well as the cellular signals controlling it. Bravo was identified with monoclonal antibodies from a collection generated against exposed molecules isolated by using a selective cell surface biotinylation procedure. The NH2-terminal sequence of Bravo shows similarity with L1, a neural surface molecule which is a member of the immunoglobulin superfamily. This possible relationship to L1, together with its restricted appearance, suggests an involvement of Bravo in axonal growth and guidance

Procedimento para análise lignocelulósica.

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Procedimentos para análise lignocelulósica.

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Switching of Magnetic Moments of Nanoparticles by Surface Acoustic Waves

We report evidence of the magnetization reversal in nanoparticles by surface acoustic waves (SAWs). The experimental system consists of isolated magnetite nanoparticles dispersed on a piezoelectric substrate. Magnetic relaxation from a saturated state becomes significantly enhanced in the presence of the SAW at a constant temperature of the substrate. The dependence of the relaxation on SAW power and frequency has been investigated. The effect is explained by the effective ac magnetic field generated by the SAW in the nanoparticles.Comment: Accepted in Europhysics Letter