Residual Action of Slow Release Systemic Insecticides on \u3ci\u3eRhopalosiphum Padi\u3c/i\u3e (Homoptera: Aphididae) on Wheat

Slow release formulations of acephate and carbofuran encapsulated in pearl corn­ starch or corn flour granules were applied to the soil at seeding time of potted \u27Caldwell\u27 wheat in the laboratory. Dosages of these insecticides were adjusted to a standard of IO kg/ha of a 10 10 granular formulation of carbofuran. The residual action of these insecticide treatments against Rhopalosiphum padi were compared with those obtained with that of carbofuran 150 at corresponding dosages and foliar sprays of solutions of acephate (25 10 EC) at 0.2 10 and carbofuran (4F) at 1.25 10, applied 12 d after seedling emergence. The residual action of carbofuran 150, which controlled R. padi since seedling emergence, lasted 28.5 d. The slow release granular formulations of carbofuran began to provide control (\u3e 50 10 aphid mortality) on days 13.3 and 17.9 after seeding. They controlled the insect until days 31.6 and 35.5 after seeding. The two corresponding granular formulations of acephate began to provide control on days 15.0 and 17.0 after seeding and con­ trolled the aphids until days 31.5 and 32.8 after seeding. The foliar sprays of acephate and carbofuran provided control for 18.3 and 36.2 d from application, respectively. The slow release granular formulations provided control of R. padi, an important vector of barley yellow dwarf virus, during early. stages of wheat development

Does functional soil microbial diversity contribute to explain within-site plant beta-diversity in an alpine grassland and a <i>dehesa</i> meadow in Spain?

Questions: Once that the effects of hydrological and chemical soil properties have been accounted for, does soil microbial diversity contribute to explain change in plant community structure (i.e. within-site beta-diversity)? If so, at which spatial scale does microbial diversity operate? Location: La Mina in Moscosa Farm, Salamanca, western Spain (dehesa community) and Laguna Larga in the Urbión Peaks, Soria, central-northern Spain (alpine grassland). Methods: The abundance of vascular plant species, soil gram-negative microbial functional types and soil chemical properties (pH, available phosphorus, and extractable cations) were sampled at both sites, for which hydrological models were available. Redundancy analysis (RDA) was used to partition variation in plant community structure into hydrological, chemical and microbial components. Spatial filters, arranged in scalograms, were used to test for the spatial scales at which plant community structure change. Results: In the case of the dehesa the diversity of soil gram-negative microbes, weakly driven by soil pH, contributed to a small extent (adj-R2 = 2%) and at a relative medium spatial scale to explain change in plant community structure. The abundance of a few dehesa species, both annual (Trifolium dubium, Vulpia bromoides) and perennial (Poa bulbosa, Festuca ampla), was associated with either increasing or decreasing soil microbial diversity. In the alpine meadow the contribution was negligible. Conclusions: Microbial diversity can drive community structure, though in the hierarchy of environmental factors structuring communities it appears to rank lower than other soil factors. Still, microbial diversity appears to promote or restrain individual plant species. This paper aims to encourage future studies to use more comprehensive and insightful techniques to assess microbial diversity and to combine this with statistical approaches such as the one used here

Molecular Diagnostics in the Mycosphaerella Leaf Spot Disease Complex of Banana and for Radopholus similis

Mycosphaerella leaf spots and nematodes threaten banana cultivation worldwide. The Mycosphaerella disease complex involves three related ascomycetous fungi: Mycosphaerella fijiensis, M. musicola and M. eumusae. The exact distribution of these three species and their disease epidemiology remain unclear, since their symptoms and life cycles are rather similar. Diagnosing these diseases and the respective causal agents is based on the presence of host symptoms and fungal fruiting structures, but is time consuming and not conducive to preventive management. In the present study, we developed rapid and robust species-specific diagnostic tools to detect and quantify M. fijiensis, M. musicola and M. eumusae. Conventional species-specific PCR primers were developed based on the actin gene that detected as little as 100, 1 and 10 pg/µl DNA from, respectively, M. fijiensis, M. musicola and M. eumusae. Furthermore, TaqMan real-time quantitative PCR assays that were developed based on the ß-tubulin gene detected quantities as low as 1 pg/µl DNA of each species from pure cultures and 1.6 pg/µl DNA/mg of M. fijiensis from dry leaf tissue. The efficacy of the tests was validated using naturally infected banana leaves. Similar technology has been used to develop a quantitative PCR assay for the banana burrowing nematode, Radopholus similis, which is currently being validate

Distorted cyclotron line profile in Cep X-4 as observed by NuSTAR

We present spectral analysis of NuSTAR and Swift observations of Cep X-4 during its outburst in 2014. We observed the source once during the peak of the outburst and once during the decay, finding good agreement in the spectral shape between the observations. We describe the continuum using a powerlaw with a Fermi-Dirac cutoff at high energies. Cep X-4 has a very strong cyclotron resonant scattering feature (CRSF) around 30 keV. A simple absorption-like line with a Gaussian optical depth or a pseudo-Lorentzian profile both fail to describe the shape of the CRSF accurately, leaving significant deviations at the red side of the line. We characterize this asymmetry with a second absorption feature around 19 keV. The line energy of the CRSF, which is not influenced by the addition of this feature, shows a small but significant positive luminosity dependence. With luminosities between (1-6)e36 erg/s, Cep X-4 is below the theoretical limit where such a correlation is expected. This behavior is similar to Vela X-1 and we discuss parallels between the two systems.Comment: 6 pages, 4 figure, accepted for publication in ApJ letter

Densitometry and Thermometry of Starburst Galaxies

With a goal toward deriving the physical conditions in external galaxies, we present a survey of formaldehyde (H2CO) and ammonia (NH3) emission and absorption in a sample of starburst galaxies using the Green Bank Telescope. By extending well-established techniques used to derive the spatial density in star formation regions in our own Galaxy, we show how the relative intensity of the 1(10)-1(11) and 2(11)-2(12) K-doublet transitions of H2CO can provide an accurate densitometer for the active star formation environments found in starburst galaxies (c.f. Mangum et al. 2008). Similarly, we employ the well-established technique of using the relative intensities of the (1,1), (2,2), and (4,4) transitions of NH3 to derive the kinetic temperature in starburst galaxies. Our measurements of the kinetic temperature constrained spatial density in our starburst galaxy sample represent the first mean density measurements made toward starburst galaxies. We note a disparity between kinetic temperature measurements derived assuming direct coupling to dust and those derived from our NH3 measurements which points to the absolute need for direct gas kinetic temperature measurements using an appropriate molecular probe. Finally, our spatial density measurements point to a rough constancy to the spatial density (10^{4.5} to 10^{5.5} cm^{-3}) in our starburst galaxy sample. This implies that the Schmidt-Kennicutt relation between L_{IR} and M_{dense}: (1) Is a measure of the dense gas mass reservoir available to form stars, and (2) Is not directly dependent upon a higher average density driving the star formation process in the most luminous starburst galaxies.Comment: 4 pages, to appear in proceedings of The 5th Zermatt ISM Symposiu

Caracteres preimaginales y aspectos bionómicos de Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae)

Se describe la morfología y microestructura coriónica del huevo y la morfología del primer estadio larvario de Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae, Nycteliini). También se exponen antecedentes bionómicos y distribucionales de la especie. Para la obtención de huevos y larvas, se recolectaron parejas en el terreno que fueron mantenidas en cajas de cria hasta la ovoposición y posterior eclosión. La estructura y ornamentación del exocorion del huevo y características morfológicas externas de la larva fueron analizadas mediante microscopía electrónica de barrido. Los resultados muestran que los huevos de G. luczotii presentan un micropilo redondeado y exocorion liso, con células hexagonales sin aeropilos. Las larvas de G. luczotii presentan características morfológicas adaptativas para la vida edáfica del tipo Pedobionta: cápsula cefálica con gran cantidad de sensillas, patas protorácicas de función cavadora, y pigopodio bien desarrollado. Se analiza la importancia de algunos caracteres morfológicos de la larva de primer estadio como criterio de diagnóstico específico y se establece que las diferencias interespecíficas referidas a las sensillas frontales, la forma del clípeo y el margen anterior del labro tienen valor taxonómico y probablemente filogenético. Palabras clave: Tenebrionidae, Gyriosomus, Morfología, Estadios preimaginales, Desierto costero, Chile.Preimaginal characters and bionomical aspects of Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae) We describe the morphology and microstructure of the egg chorion, and the morphology of the first instar larva of Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae, Nycteliini). Bionomical and distributional data on this species are also provided. To obtain eggs and larvae, couples were collected in the field and kept in rearing cages until oviposture and ecclosion. The structure and adornment of the egg exochorion, and the exterior morphological features of larvae were examined with electron scanning microscopy. The eggs of G. luczotii showed a rounded micropyle and a smooth exochorion, composed of hexagonal cells without aeropyles. The larvae of G. luczotii showed morphological characteristics suited for an edaphic life similar to that of Pedobionta: digging prothoracic legs, cephalic capsule with abundant sensilla, and well–developed pygopodium. We analysed the importance of larval morphology as an element for specific diagnosis and found that interspecific differences regarding frontal sensilla, clypeus shape, and anterior part of labrum, had a taxonomic value and possibly a phylogenetic value. Key words: Tenebrionidae, Gyriosomus, Morphology, Preimaginal stages, Coastal desert, Chile.Se describe la morfología y microestructura coriónica del huevo y la morfología del primer estadio larvario de Gyriosomus luczotii Laporte, 1840 (Coleoptera, Tenebrionidae, Nycteliini). También se exponen antecedentes bionómicos y distribucionales de la especie. Para la obtención de huevos y larvas, se recolectaron parejas en el terreno que fueron mantenidas en cajas de cria hasta la ovoposición y posterior eclosión. La estructura y ornamentación del exocorion del huevo y características morfológicas externas de la larva fueron analizadas mediante microscopía electrónica de barrido. Los resultados muestran que los huevos de G. luczotii presentan un micropilo redondeado y exocorion liso, con células hexagonales sin aeropilos. Las larvas de G. luczotii presentan características morfológicas adaptativas para la vida edáfica del tipo Pedobionta: cápsula cefálica con gran cantidad de sensillas, patas protorácicas de función cavadora, y pigopodio bien desarrollado. Se analiza la importancia de algunos caracteres morfológicos de la larva de primer estadio como criterio de diagnóstico específico y se establece que las diferencias interespecíficas referidas a las sensillas frontales, la forma del clípeo y el margen anterior del labro tienen valor taxonómico y probablemente filogenético. Palabras clave: Tenebrionidae, Gyriosomus, Morfología, Estadios preimaginales, Desierto costero, Chile