Grenzueberschreitende binationale Umwelterziehung in der Agrarausbildung. Projektband und Bd. 1: Das Grundmodul. Bd. 2: Die Aufbaumodule

Published in 3 volumesSIGLEAvailable from TIB Hannover: F02B1040: F02B1041: F02B1042 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDeutsche Bundesstiftung Umwelt, Osnabrueck (Germany)DEGerman

Dielectrophoretic-activated cell sorter based on curved microelectrodes

This article presents the numerical and experimental analysis of a dielectrophoretic-activated cell sorter (DACS), which is equipped with curved microelectrodes. Curved microelectrodes offer unique advantages, since they create strong dielectrophoretic (DEP) forces over the tips and maintain it over a large portion of their structure, as predicted by simulations. The performance of the system is assessed using yeast (Saccharomyces cerevisiae) cells as model organisms. The separation of the live and dead cells is demonstrated at different medium conductivities of 0.001 and 0.14 S/m, and the sorting performance was assessed using a second array of microelectrodes patterned downstream the microchannel. Further, microscopic cell counting analysis reveals that a single pass through the system yields a separating efficiency of ~80% at low medium conductivities and ~85% at high medium conductivities.<br /

Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, and DNA Stretching

matlab files for generating the explanations in section 4.Dorfman, Kevin D.; King, Scott B; Olson, Daniel W.; Thomas, Joel DP; Tree, Douglas R. (2013). Data from: Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis and DNA Stretching. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/155356